FAMILIAL LACK OF THE IgG3 SUBCLASS: GENE ELIMINATION OR TURNING OFF EXPRESSION AND NEUTRAL EVOLUTION IN THE IMMUNE SYSTEM

Lefranc, G.; Dumitresco, S.M.; Salier, J.P.; Rivat, L; Lange, Gerda de; Loghem, Erna van; Loiselet, Jacques

doi:10.1111/j.1744-313x.1979.tb00679.x

Cited by 32 publications

(16 citation statements)

References 28 publications

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“…The pass-through after protein A adsorp family studies [2,6,[15][16][17]. Despite the evi dence for rare cross-overs between the cistrons coding for the y-subclass heavy chains, their close linkage has been more striking.…”

Section: Protein a Chromatographymentioning

confidence: 99%

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Human IgG Allotypes Co-Occurring in More than One IgG Subclass

Loghem¹,

Lange²,

Leeuwen³

et al. 1982

Vox Sanguinis

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Inheritance of an excess of immunoglobulin allotypes in one haplotype was encountered which could not be explained by the assumption of a duplicated locus. The surplus of allotypes was related to markers on the CH3 domain of y3 chains. Two such cases were investigated extensively. The IgG3 molecules were isolated by gel filtration and by absorption on protein A. Only the usual combination of allotypes appeared to be present on the IgG3 molecules. The supernumerary markers were found in one case on IgG2 molecules and in the other case on IgG 1 molecules. This followed from investigations of eluates after separation of the subclasses by immune absorptions. A hypothesis was proposed to explain these events by mutation of a particular position of an otherwise homologous stretch of y-subclass DNA.

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Section: Protein a Chromatographymentioning

confidence: 99%

“…Racial differences and uncommon genes or haplotypes have been attributed to intergenic or intragenic crossing-over of estab lished haplotypes or to a point mutation within a particular locus [2,12,15,16].…”

Section: Introductionmentioning

confidence: 99%

Human IgG Allotypes Co-Occurring in More than One IgG Subclass

Loghem¹,

Lange²,

Leeuwen³

et al. 1982

Vox Sanguinis

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“…Selective absence of single IgG subclasses have been rarely identified by immunological testing for allo-and isotype determinants (12)(13)(14). Until now, the only cases of multiple IgCH gene deletions were found in apparently healthy individuals from highly inbred communities of Tunisian Berbers (23)(24)(25).…”

Section: Resultsmentioning

confidence: 99%

“…Selective deficiencies of Ig subclasses have occasionally been found, even in apparently healthy individuals (12)(13)(14). Two questions are raised by these findings: the first deals with the molecular basis of the lack of Ig chains, and the second is related to how and to what extent the lost effector functions are compensated.…”

mentioning

confidence: 99%

Multiple gene deletions within the human immunoglobulin heavy-chain cluster.

Migone¹,

Oliviero²,

Lange³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

106

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Two subjects, of 11,000 healthy individuals screened, were found to be missing three and four immunoglobulin isotypes, respectively (IgAl, IgG2, and IgG4; IgAl, IgG2, IgG4, and IgE), and have beei analyzed at the DNA level by means of Southern blotting and Ig heavy-chain-specific probes. A broad deletion within the heavy-chain constant region (C) gene cluster was found on chromosome 14 of both probands. Two different haplotypes are described: the first has lost the Cai,, Cty, Cy2, Cy4, and CE genes; the second lacks the Cp,> Ca., Cy,1 C.,2, andhCy4 genes. These findings confirm the reciprocal order of the Ig heavy-chain genes as derived by molecular cloning. The inclusion of the C*, gene within the deleted regions confirms its location between Ca, and Cy2.From the observed frequency of the homozygous genotype, 1%-3% of healthy subjects from our population are expected to be heterozygous for multiple heavy-chain gene deletions. Cross-over between mispaired homologous regions seems to be the favored mechanism of multiple Ig gene deletions and duplications, and, generally, in the evolution of the human Ig heavy-chain gene family.

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“…On the basis of the restriction maps of cloned Cy genes, the 7.8 kb band can be assigned to the $y [28], 71 and 73 [7] genes, the 6.7 and 6.3 kb respectively to the ~4 and y2 genes [6,7]. [23,24]; 3, BAR 11-3, heterozygous for the Gm 3Z23;-A2m' haplotype [23,24]. Hybridization of the pSr3h probe to BAR III-6 DNA shows that the selective absence of IgG3 is not due to a deletion of the Cy3 gene.…”

Section: Familial Lack Of Igg3mentioning

confidence: 99%

A gamma 3 hinge region probe: first specific human immunoglobulin subclass probe

Huck¹,

Keyeux²,

Ghanem³

et al. 1986

FEBS Letters

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We report the first specific human immunoglobulin subclass probe which was obtained by subcloning the gamma 3 hinge region. This specific y3 probe allowed us to identify with certainty the Cy3 gene on Southern genomic blots, to describe the first Cy3 restriction fragment length polymorphism (EZZ y3 RF) and to show that an IgG3 selective deficiency, previously described serologically, was not due to a deletion of the Cy3 gene. Such a probe should be particularly useful for screening libraries from individuals with IgG3 immunodeficiencies or presenting unusual Cy3 genes and, consequently, for studying the Cy gene evolution.

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FAMILIAL LACK OF THE IgG3 SUBCLASS: GENE ELIMINATION OR TURNING OFF EXPRESSION AND NEUTRAL EVOLUTION IN THE IMMUNE SYSTEM

Cited by 32 publications

References 28 publications

Human IgG Allotypes Co-Occurring in More than One IgG Subclass

Human IgG Allotypes Co-Occurring in More than One IgG Subclass

Multiple gene deletions within the human immunoglobulin heavy-chain cluster.

A gamma 3 hinge region probe: first specific human immunoglobulin subclass probe

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