Failure of Aphids to Transmit the Odontoglossum Ringspot and Cymbidium Mosaic Viruses to Orchid Plantlets Derived from Meristem Cultures

Namba, R.; Ishii, Minoru

doi:10.1094/phyto-61-582

Cited by 14 publications

(4 citation statements)

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“…They can be transmitted mechanically via inoculation of infected plant sap, or through contaminated equipment such as cutting tools. In addition, these viruses are not transmitted by seeds or vectors 2 , 3 . CymMV induces chlorotic or necrotic patches on the leaves and the flowers of orchids.…”

Section: Introductionmentioning

confidence: 99%

Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants

Petchthai

Yee

Wong

2018

Sci Rep

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Transgenic plants expressing artificial microRNAs (amiRNAs) have been shown to confer specific resistance to corresponding viruses. Here, we generated Nicotiana benthamiana transgenic lines containing Oryza sativa miR528 as backbone, expressing amiRNAs targeting RNA-dependent RNA polymerase (RdRp) gene of Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). The amiRNA transgenic lines could express amiR-CymMV and confer high percentage resistance to CymMV, while lack of detectable level of amiR-ORSV expression in amiR-ORSV transgenic N. benthamiana plants led to weak resistance to ORSV infection. In this project, we provide the first report of CymMV-resistant transgenic N. benthamiana plants based on amiRNA strategy. We believe that this amiRNA approach can be extended to generate CymMV-resistant transgenic orchids.

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Section: Introductionmentioning

confidence: 99%

Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants

Petchthai

Yee

Wong

2018

Sci Rep

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“…Their stable particles are able to maintain infectivity during long periods in the plant sap (Wisler, 1989), and eventually can be transmitted by contaminated pots and soil (Loebenstein et al 1995). There is no evidence of vectors involved in the transmission of these two viruses (Namba and Ishii 1971). Another widespread orchid virus is Orchid fleck dichorhavirus (OFV), a member of the Dichorhavirus genus in the family Rhabdoviridae (Kondo et al 2006) which is transmitted by the false-spider mite Brevipalpus californicus (Banks) in a persistent manner (Kondo et al 2003).…”

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confidence: 99%

Incidence and characterization of viruses infecting orchids in São Paulo state, Brazil

Moraes

Krause‐Sakate

Pavan

2017

Trop. plant pathol.

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Orchids are susceptible to a variety of abiotic and biotic agents that can affect their development and decrease the quality of their flowers and leaves. Viruses are one of the most important biotic agents, with Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) as the most prevalent and of greater economic importance worldwide. Similar to a survey of virus infection in orchids carried out 20 years ago by Freitas et al. in the state of São Paulo, a new evaluation was made incorporating molecular methodologies on a total of 385 samples collected from commercial growers. Commercial antisera against CymMV, ORSV, Cymbidium ringspot virus, Tomato spotted wilt virus and viruses in the genus Potyvirus were used in ELISA tests. PCRs were performed to detect Orchid fleck virus (OFV) and primers were designed to amplify the coding region of the CymMV and ORSV capsid proteins. The most prevalent virus was CymMV, present in 274 samples (71.16%), followed by ORSV and OFV, present in 61 and 11 samples (15.85% and 2,85%), respectively. CymMV and ORSV capsid protein genes showed high sequence conservation. Symptoms caused by CymMV and ORSV have a great variability and are commonly confused with abiotic factors, nutritional problems and phytotoxicity.

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“…It is reported to be transmitted through pollen in some species (Namba and Ishii, 1971; Yuen et aI., 1979;Michon, 1982; Hamilton and Valentine, 1984; Hu et aI., 1994). Non-vector transmission is by mechanical inoculation and by contact between plants.…”

Section: Literature Citedmentioning

confidence: 99%

Orchid Biology: Reviews and Perspectives, VIII

Comber¹,

Hew²

2002

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Failure of Aphids to Transmit the Odontoglossum Ringspot and Cymbidium Mosaic Viruses to Orchid Plantlets Derived from Meristem Cultures

Cited by 14 publications

References 0 publications

Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants

Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants

Incidence and characterization of viruses infecting orchids in São Paulo state, Brazil

Orchid Biology: Reviews and Perspectives, VIII

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