Faecal shedding of pathogenic Yersinia enterocolitica determined by qPCR for yst virulence gene is associated with reduced live weight but not diarrhoea in prime lambs

Jacobson, Caroline; Yang, Rongchang; Williams, A W; Gardner, G.E.; Carmichael, Ian; Campbell, Angus; Ryan, Una

doi:10.1016/j.prevetmed.2018.02.004

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Compared with PCR, the amplification process of qPCR is presented in real time [ 19 ], and it can provide more robust, specific, and sensitive quantitative results. Based on these characteristics of qPCR, it has been used for the detection of Y. enterocolitica in feces and food [ 20 , 21 ]. RPA is a recent isothermal nucleic acid amplification technology, and its development in molecular diagnostics is often motivated by its simplicity.…”

Section: Introductionmentioning

confidence: 99%

Ultra-Sensitive and Rapid Detection of Pathogenic Yersinia enterocolitica Based on the CRISPR/Cas12a Nucleic Acid Identification Platform

Xiao

Ren

et al. 2022

Foods

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Yersinia enterocolitica is a dangerous foodborne human pathogen that mainly causes gastroenteritis. Ideal methods for the detection of pathogens in food should be rapid, sensitive, specific, and cost effective. To this end, novel in vitro nucleic acid identification methods based on clustered, regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) endonuclease have received increasing attention. In this study, a simple, visual, and ultrasensitive method, based on CRISPR/Cas12a with recombinase polymerase amplification (RPA), was developed for the detection of Y. enterocolitica. The results show that a specific attachment invasion locus gene (ail) can be rapidly detected using a CRISPR/Cas12a-RPA-based system. Application of the method to raw pork, which was artificially infected with Y. enterocolitica, achieved an estimated detection limit of 1.7 CFU/mL in less than 45 min, and this was 100 times lower compared with qPCR. The results indicated that the CRISPR/Cas12a-RPA system has good potential for monitoring pathogenic Y. enterocolitica in the chilled meat supply chain.

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Section: Introductionmentioning

confidence: 99%