Faecal proteomics in the identification of biomarkers to differentiate canine chronic enteropathies

O'Reilly, Emily; Horvatić, Anita; Kuleš, Josipa; Gelemanović, Andrea; Mrljak, Vladimir; Huang, Yi-Xin; Brady, Nicola; Chadwick, Christopher C.; Eckersall, P.D.; Ridyard, Alison

doi:10.1016/j.jprot.2021.104452

Cited by 8 publications

(20 citation statements)

References 42 publications

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“…The main limitations of the present study are: (i) the possibility of comparing our results with the very few previous investigations already present in the literature [ 7 , 8 , 9 , 20 ], only one of which concerns felids (i.e., cats) [ 7 ]; and (ii) the low case load reported herein. Which species is being described must be considered, as well as how difficult it could be to include such patients.…”

Section: Discussionmentioning

confidence: 72%

“…This protein was also reported in a study conducted in 2017 involving the protein profiles of the fecal material of female cheetahs [ 18 ] and pregnant polar bears [ 19 ]. Moreover, in recent studies on the fecal proteome, it has been found to be more abundant in dogs presenting with canine chronic enteropathy (especially in non-food-responsive chronic inflammatory enteropathy) than in healthy controls [ 20 ], as well as in dogs presenting intestinal lymphangiectasia [ 9 ]. In the light of these published data, it is difficult to interpret our finding in healthy cheetahs; additionally, this is because a further study published on fecal proteomics similarly found this protein (more precisely, a transthyretin precursor) in the feces of healthy cats [ 7 ], leading to the consideration that this protein could be a normal finding in both healthy and diseased patients.…”

Section: Discussionmentioning

confidence: 99%

“…It was found to be increased in the intestine of human patients affected by ulcerative colitis and Crohn’s disease [ 25 ] and to be more abundant in pregnant polar bears’ feces [ 19 ]. The protein was also found in dogs suffering from lymphangiectasia and in healthy cats [ 7 , 9 ]; on the contrary, it was not described in healthy dogs and in dogs with chronic enteropathy [ 7 , 20 ]. Unfortunately, the few and hardly matching data available in the literature did not allow for an in-depth interpretation of our findings.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Fecal Proteome in Healthy and Diseased Cheetahs (Acinonyx jubatus) Suffering from Gastrointestinal Disorders

Mangiaterra

Vincenzetti²,

Rossi³

et al. 2022

Animals

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Fecal proteomics allows for the identification of proteins and peptides present in stools and is useful in finding possible new biomarkers for diagnosing and/or monitoring gastrointestinal (GI) disorders. In the present study, we investigated the fecal proteome in healthy and diseased cheetahs (Acinonyx jubatus). Captive individuals of this species frequently show gastrointestinal disorders characterized by recurrent episodes of diarrhea, rare episodes of vomiting and weight loss, associated with Helicobacter spp. infection. Fecal proteomic evaluation has been performed by two-dimensional electrophoresis followed by liquid chromatography-tandem mass spectrometry. In healthy cheetahs, the results showed the presence of the following proteins: collagen alpha-1 (II) chain, transthyretin, IgG Fc-binding protein, titin, dystonin, isopentenyl-diphosphate Delta-isomerase 1, sodium/potassium-transporting ATPase subunit alpha-1 and protein disulfide-isomerase A6. The presence of albumin isoforms was found only in diseased cheetahs. The present paper reports the study of the fecal proteome in the cheetah, evidences some differences between healthy and diseased patients and confirms, once again, the potential of fecal proteomics for the study of the GI environment, with promising developments regarding the identification of new diagnostic/monitoring markers.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Fecal Proteome in Healthy and Diseased Cheetahs (Acinonyx jubatus) Suffering from Gastrointestinal Disorders

Mangiaterra

Vincenzetti²,

Rossi³

et al. 2022

Animals

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“…In order to avoid misinterpretation as orthologs, the designations REG3E1 and REG3E2 were assigned to the canine genes by the Vertebrate Gene Nomenclature Committee. Given previous entries on NCBI and Ensembl designating REG3E1 as REG3A , it is reasonable to assume that O'Reilly and colleagues have in fact identified REG3E in canine feces ( 31 ). Similar to reports in humans, where REG3A is upregulated in inflammatory bowel disease ( 19 ), the afore mentioned study found increased amounts of REG3A in the feces of dogs with chronic enteropathies compared to healthy controls, suggesting that this protein could potentially be of use in the diagnosis of canine intestinal disorders.…”

Section: Discussionmentioning

confidence: 99%

“…A homolog of REG3A was found in the intestinal mucosa of piglets ( 28 , 29 ) as well as in Peyer's plaques of the ileum and jejunum of lambs ( 30 ). Recently, REG3A was found to be upregulated in fecal samples of dogs with chronic enteropathies compared to healthy control dogs ( 31 ). To our knowledge, no further investigations into the REG family have been pursued in this species.…”

Section: Introductionmentioning

confidence: 99%

Identification of regenerating island-derived protein 3E in dogs

Peters

Howard²,

Leeb³

et al. 2022

Front. Vet. Sci.

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Regenerating islet-derived protein (REG) 1A (aka pancreatic stone protein) and REG3A (aka pancreatitis-associated protein) are upregulated in humans with sepsis, pancreatitis, and gastrointestinal diseases, but little is known about this protein family in dogs. Our aim was to identify REG1 and REG3 family members in dogs. REG-family genes were computationally annotated in the canine genome and proteome, with verification of gene expression using publicly available RNA-seq data. The presence of the protein in canine pancreatic tissue and plasma was investigated with Western blot and immunohistochemistry, using anti-human REG1A and REG3A antibodies. Protein identity was confirmed with mass spectrometry. Two members of the REG3 subfamily were found in the canine genome, REG3E1 and REG3E2, both encoding for the same 176 AA protein, subsequently named REG3E. Anti-human REG3A antibodies demonstrated cross-reactivity with the canine REG3E protein in pancreas homogenates. In canine plasma, a protein band of approximately 17 kDa was apparent. Mass spectrometry confirmed this protein to be the product of the two annotated REG3E genes. Strong immunoreactivity to anti-human REG3A antibodies was found in sections of canine pancreas affected with acute pancreatitis, but it was weak in healthy pancreatic tissue. Recombinant canine REG3E protein underwent a selective trypsin digestion as described in other species. No evidence for the presence of a homolog of REG1A in dogs was found in any of the investigations. In conclusion, dogs express REG3E in the pancreas, whose role as biomarker merits further investigations. Homologs to human REG1A are not likely to exist in dogs.

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Serum proteome of dogs with chronic enteropathy

Ruaux

Griebsch

et al. 2023

Veterinary Internal Medicne

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Background: Chronic enteropathy (CE) is common in dogs and can occur with multiple etiologies including food-responsive enteropathy (FRE) and idiopathic inflammatory bowel disease (IBD).Hypothesis/Objective: To study the protein profile and pathway differences among dogs with FRE, IBD, and healthy controls using serum proteome analysis.Animals: Nine CE dogs with signs of gastrointestinal disease and histologically confirmed chronic inflammatory enteropathy and 16 healthy controls. Methods:A cross-sectional study with cases recruited from 2 veterinary hospitals between May 2019 and November 2020 was performed. Serum samples were analyzed using mass spectrometry-based proteomic techniques.Results: Proteomic profiles showed marked variation in relative protein abundances.Forty-five proteins were significantly (P ≤ .01) differentially expressed among the dogs with CE and controls with ≥2-fold change in abundance. The fold change of dogs with IBD normalized to controls was more pronounced for the majority of proteins than that seen in the dogs with FRE normalized to control dogs. Proteins involving reactive oxygen species, cytokine activation, acute phase response signaling, and lipid metabolism were altered in dogs with CE.Conclusions and Clinical Importance: Cytokine alterations, acute phase response signaling, and lipid metabolism are likely involved in pathogenesis of CE. Although there are insufficient current data to justify the use of proteomic biomarkers for assessment of CE in dogs, our study identifies potential candidates.

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Faecal proteomics in the identification of biomarkers to differentiate canine chronic enteropathies

Cited by 8 publications

References 42 publications

Evaluation of the Fecal Proteome in Healthy and Diseased Cheetahs (Acinonyx jubatus) Suffering from Gastrointestinal Disorders

Evaluation of the Fecal Proteome in Healthy and Diseased Cheetahs (Acinonyx jubatus) Suffering from Gastrointestinal Disorders

Identification of regenerating island-derived protein 3E in dogs

Serum proteome of dogs with chronic enteropathy

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