Evaluation of the Fecal Proteome in Healthy and Diseased Cheetahs (Acinonyx jubatus) Suffering from Gastrointestinal Disorders

Mangiaterra, Sara; Vincenzetti, Silvia; Rossi, Giacomo; Marchegiani, Andrea; Gavazza, Alessandra; Petit, Thierry; Sagratini, Gianni; Ricciutelli, Massimo; Cerquetella, Matteo

doi:10.3390/ani12182392

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“…Proteomics is the study of proteins present in a specific biological substrate. It has been recently applied in the field of veterinary gastroenterology through the study of the fecal proteome in healthy dogs and cats, and in dogs suffering from chronic enteropathies, as well as in cheetahs [ 8 , 9 , 10 , 11 , 12 ]. In one study in cats, the gastrointestinal (GI) mucosal proteome was also characterized in patients with inflammatory bowel disease, alimentary small cell lymphoma, and healthy controls [ 13 ].…”

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confidence: 99%

Fecal Protein Profile in Eight Dogs Suffering from Acute Uncomplicated Diarrhea before and after Treatment

Cerquetella

Mangiaterra

Rossi

et al. 2023

Veterinary Sciences

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Acute diarrhea is a very frequent condition affecting dogs; nevertheless, little is known about what happens in the GI tract during such conditions. Proteomics allows the study of proteins present in a specific biologic substrate, and fecal proteomic investigations have been recently implemented to study GI diseases in dogs. In the present study, the fecal protein profiles of eight dogs suffering from acute uncomplicated diarrhea at the time of inclusion was investigated for the first time, and then the same patients were followed, replicating two further evaluations at two subsequent time points (after 2 and 14 days from the first presentation), with the aim of gaining possible new insights regarding the pathologic changes in the gastrointestinal environment during such conditions. Two-dimensional gel electrophoresis (2-DE) was performed, followed by mass spectrometry. Nine spots, corresponding to four (groups of) proteins (i.e., albumin, alkaline phosphatase, chymotrypsin-C-like, and some immunoglobulins), showed significant differences at two or more of the three time points investigated, almost all behaving similarly and decreasing at T1 (2 days after the onset of the condition) and significantly increasing at T2 (14 days after the onset), mainly evidencing a reaction of the organism. Further studies including a greater number of patients and possibly different techniques are needed to confirm the present findings.

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