Factors involved in binding of Lactobacillus plantarum Lp6 to rat small intestinal mucus

Abstract

Aim: To investigate the adhesion determinants of Lactobacillus plantarum Lp6, a dairy isolate. Methods and Results: Small intestinal mucus extracted from rats was used as a substrate for adhesion. Adhesion determinants were studied by physical, chemical and enzymatic pretreatments of the bacteria, and adhesion inhibition assay. The mannose‐specific adhesins were explored by studying the effect of d‐mannose on adhesion and the yeast‐agglutinating ability of the bacteria. It was found that adhesion decreased after bacteria were treated with sodium metaperiodate, protease K, trypsin, lithium chloride and trichloroacetic acid. However, adhesion did not decrease after trypsin‐treated bacteria were incubated with cell surface protein extract. Cell surface bound exopolysaccharides were found to inhibit the adhesion. d‐mannose inhibited the adhesion in a dose‐dependent manner. The bacteria could significantly agglutinate yeast and lost this ability after protease K treatment. Conclusions: Adhesion was mainly mediated by the mannose specific adhesins, which might be proteins that reversibly bind to the cell surface components. Cell surface‐bound exopolysaccharides were also involved in adhesion. Significance and Impact of the Study: The mannose‐specific adhesion of Lact. plantarum Lp6 to rat mucus might be important for competing with pathogens‐binding sites in gut, which may be used to resist the colonization of the pathogens.

Epithelial Adhesion Mediated by Pilin SpaC Is Required for Lactobacillus rhamnosus GG-Induced Cellular Responses

“…L. plantarum strains expressing Msa can agglutinate the yeast Saccharomyces cerevisiae, a finding that might explain how these strains competitively inhibit intestinal pathogens (35). L. plantarum Lp6 has the capacity to bind rat mucus and can also agglutinate S. cerevisiae; this strain also likely carries an msa gene since the addition of D-mannose inhibits the abovementioned properties (36). GroEL, identified in Lactobacillus johnsonii La1, binds mucus and HT29 cells in a pH-dependent manner and, similarly to Msa, has the ability to agglutinate H. pylori (37).…”

An in vitro Investigation of the Antagonistic Effects of Multiple Strains of Lactobacillales on Salmonella enterica Serovar Choleraesuis

“…Current studies have indicated that the mechanisms and related factors driving the adhesion of Lactobacillales to the epithelial cells include hydrophobic interaction, lipoteichoic acids (LTA), and exopolysaccharides [37,38]. Granato et al found that the surface of L. johnsonii cell walls contains LTA, which is also present in the supernatant of the Lactobacillales culture, and that if LTA is isolated or purified, it can inhibit cell adhesion in a dose-dependent manner [37].…”

“…Furthermore, they also found that trypsinization had an effect on the adhesion of Lactobacillales. Thus, it was observed that proteins play a role in the adhesion of L. plantarum [38].…”

PCR for Screening Potential Probiotic Lactobacilli for Piglets

“…Protein and carbohydrate play an important role in mediating the adhesion to mucosal and or epithelial host surfaces. Some cell-surface biomolecules as exopolysaccharides and proteins have been recognized by their ability to bind gastrointestinal components (Vélez et al, 2007;Rojas et al, 2002;Sun et al, 2007). The best characterized are proteins present in the surface of lactobacilli that can be attached covalently or not to the cell wall (Vélez et al, 2007).…”