“…The development of the R. necatrix mycelium and mycelial strands depends on temperature, oxygen, moisture and organic matter contents, pH and soil microflora. In vitro studies have shown that the optimal growth temperature is 22–24 °C; it does not grow below 4 °C or above 32 °C (Abe and Kono, 1953; Anselmi and Giorcelli, 1990; Araki, 1967; Mantell and Wheeler, 1973; Pérez‐Jiménez, 1997). Unlike most fungi, R. necatrix is not inhibited by high pH; in vitro growth occurs over a pH range of 4–9 (Makambila, 1978; Pérez‐Jiménez, 1997; Ruano‐Rosa, 2006), and growth in soil occurs over a pH range of 6–8 (Anselmi and Giorcelli, 1990; Gupta and Gupta, 1992).…”