Factors influencing adherence of group B streptococci to human vaginal epithelial cells

Zawaneh, Sam M.; Ayoub, Elía M.; Baer, Herman; Cruz, Amelia C.; Spellacy, William N.

doi:10.1128/iai.26.2.441-447.1979

Cited by 55 publications

(25 citation statements)

References 27 publications

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“…In both cases the organisms were killed, but the mild treatment did not reduce attachment to BEC. It appears, therefore, that viability is not a prerequisite for binding of GBS, and this finding is in agreement in with those of Zawaneh et al (1979) who showed that killing GBS with ultra-violet light or penicillin did not affect their adherence to human vaginal epithelial cells.…”

Section: Discussionsupporting

confidence: 91%

Occasional Review: Group-B Streptococcus--Profile of an Organism

Ross¹

1984

Journal of Medical Microbiology

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Introduction and historical review P. W. Ross Nocard and Mollereau (1 887) were the first to describe the isolation of streptococci from bovine mastitis and these organisms were likely to have been the group-B streptococci (GBS). Many years elapsed, however, before these streptococci featured again in the literature. In 1933, Rebecca Lancefield divided streptococci into groups on the basis of carbohydrate antigens in the cell wall; her classification scheme was based on precipitation reactions of hot-hydrochloric-acid extracts of whole organisms (Lancefield extracts) with antisera raised in rabbits against whole-cell formalinised streptococci. These classical studies clarified the taxonomy of streptococci to a considerable degree because the cultural and biochemical patterns that had been used before 1933 had added little to streptococcal identification. The streptococci that Lancefield described as group B were isolated from bovine and dairy sources. In 1934 she divided GBS into types I, I1 and I11 and in 1938 subdivided type I into Ia and Ib. All the antigens were polysaccharides. Pattison, Matthews and Maxted (1955) added to the serological profile of GBS by describing two protein antigens, R and X. They also stated that GBS from human and bovine sources were not similar and this was corroborated by Butter and de Moor (1967). Wilkinson (1975) discovered a third protein antigen and called it Icp. It is generally referred to as the Ic protein and is usually found in strains with the Ia or Ib polysaccharide. Strains possessing the Ia and Ic antigens are said to belong to type Ic. The Icp antigen may be found in strains with the I1 or the I11 polysaccharide or without a detectable polysaccharide antigen. With serological tests that identify carbohydrate and protein antigens, many combinations of antigens are found-Ia, Ib, Ic, Icp, 11,

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Section: Discussionsupporting

confidence: 91%

Occasional Review: Group-B Streptococcus--Profile of an Organism

Ross¹

1984

Journal of Medical Microbiology

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“…We investigated the role of pH because adherence of bacteria to VECs has been shown to be pH dependent (38,52) and because adherence of T. vaginalis to cell lines is pH dependent (3). Interestingly, maximal adherence of T. foetus to VECs was at pH 6.0 to 7.5, approximately the pH range of the bovine vagina.…”

Section: Discussionmentioning

confidence: 99%

Adherence of Tritrichomonas foetus to bovine vaginal epithelial cells

et al. 1989

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Adherence of Tritrichomonas foetus to bovine vaginal epithelial cells (VECs) in vitro was investigated with fresh washed bovine VECs and log-phase cultures of T. foetus. Observation under phase-contrast microscopy showed that T. foetus usually adhered first by the posterior flagellum and later by the body. Significantly more keratinized squamous epithelial cells were detected with attached parasites than nonkeratinized round epithelial cells. The optimal pH range for attachment was 6.0 to 7.5, with peak attachment at pH 6.5 for squamous VECs. Surface-reactive bovine antiserum to T. foetus prevented adherence to bovine squamous VECs. Inhibition of adherence occurred at nonagglutinating, nonimmobilizing serum dilutions. Antiserum fractions enriched for immunoglobulin Gl inhibited adherence, but fractions enriched for immunoglobulin G2 did not. The inhibitory antiserum was specific for several mediumto high-molecular-weight membrane antigens as detected in Western blots (immunoblots). The ability of surface-reactive antibodies to prevent adherence and to agglutinate and immobilize T. foetus indicates that they may be protective.

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“…It has been reported that the presence of saliva increases adhesion of C. albicans to buccal cells [5] and other secretions are likely to play a role in adhesion (vaginal secretions, intestinal mucus etc). Moreover, some bacteria adhere better at low pH [1,6,7] while others adhere better at neutral pH [8].…”

Section: Resultsmentioning

confidence: 99%

Possible role of hormones in the observed changes in adhesion of several microorganisms to epithelial cells from different body sites

Botta

1981

FEMS Microbiology Letters

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Factors influencing adherence of group B streptococci to human vaginal epithelial cells

Cited by 55 publications

References 27 publications

Occasional Review: Group-B Streptococcus--Profile of an Organism

Occasional Review: Group-B Streptococcus--Profile of an Organism

Adherence of Tritrichomonas foetus to bovine vaginal epithelial cells

Possible role of hormones in the observed changes in adhesion of several microorganisms to epithelial cells from different body sites

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