Possible role of hormones in the observed changes in adhesion of several microorganisms to epithelial cells from different body sites

Botta, Giuseppe

doi:10.1111/j.1574-6968.1981.tb06937.x

Cited by 22 publications

(1 citation statement)

References 12 publications

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“…albicans can adhere to a variety of epithelial cell types in addition to buccal cells. These include vaginal (King et al, 1980;Sobel et al, 1981) and uro-epithelial cells (Botta, 1981;Centeno et al, 1983), as well as epidermal corneocytes (Botta, 1981 ;Ray et al, 1984;Collins-Lech et al, 1984). Crude EP inhibited adhesion to mouse vaginal cells and the extent of inhibition observed was similar to that obtained with buccal cells.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Partial Characterization of an Adhesin from Candida albicans

Critchley

Douglas²

1987

Microbiology

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Candida albicans produces extracellular polymeric material (EP) which contains a mannoprotein adhesin. EP isolated from culture supernatants of C. albicans GDH 2346 consisted of a mixture of glycoprotein components and inhibited yeast adhesion to buccal epithelial cells by up to 60%. Partial purification of the adhesin was achieved by a two-step procedure involving chromatography of EP on concanavalin A-Sepharose and DEAE-cellulose. The purified adhesin inhibited adhesion to buccal cells 30 times more efficiently (on a weight basis) than unfractionated EP. Pretreatment of EP with heat, dithiothreitol or proteolytic enzymes either partially or completely destroyed its ability to inhibit adhesion, whereas pretreatment with sodium periodate or alpha-mannosidase had little or no effect. These results suggest that the protein portion of the mannoprotein adhesin is more important than the carbohydrate moiety in mediating yeast attachment to buccal epithelial cells.

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Section: Discussionmentioning

confidence: 99%

Isolation and Partial Characterization of an Adhesin from Candida albicans

Critchley

Douglas²

1987

Microbiology

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Adherence of Candida albicans: Influencing Factors and Mechanism(s)

Ghannoum

Abu-Elteen

1991

Candida Albicans

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Influence of mucosal cell origin on the in vitro adherence of Candida albicans: Are mucosal cells from different sources equivalent?

et al. 1987

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The influence of collecting mucosal cells from various anatomical sites, and varying the date of collection and cell donor on adhesion of Candida albicans to human epithelial cells was examined by using an in vitro adherence assay. Examination of buccal mucosal cells from twenty-four donors showed statistically significant differences in the number of attached yeasts between individuals. Sex did not exert a significant influence on adhesion. Examination of buccal mucosal cells from ten donors collected on five different dates revealed that yeast attachment to mucosal epithelial cells varied significantly within subjects across time. Epithelial cells from some donors manifested greater date-to-date variations in yeast adhesion than others. Adherence of Candida to mucosal cells from three anatomical sites (mouth, vagina and urinary tract) collected from ten different donors was also tested. Yeast adherence to buccal cells was highest, lowest using urinary tract cells, while vaginal epithelium was intermediate. Adherence to mucosal cells from three sites was significantly different both within and between individuals although some subjects manifested larger variations than others. These data suggest that the in vitro adherence of Candida albicans is influenced by mucosal cell donor, date of collection and body site of origin. Mucosal cells from different sources do not appear to be equivalent in receptiveness to C. albicans and this might explain some of the discrepancies observed when adhesion studies performed by different investigators are compared. The existing need for a more uniform methodology with which to pursue studies on fungal attachment to mucosal surfaces is emphasized.

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Possible role of hormones in the observed changes in adhesion of several microorganisms to epithelial cells from different body sites

Cited by 22 publications

References 12 publications

Isolation and Partial Characterization of an Adhesin from Candida albicans

Isolation and Partial Characterization of an Adhesin from Candida albicans

Adherence of Candida albicans: Influencing Factors and Mechanism(s)

Influence of mucosal cell origin on the in vitro adherence of Candida albicans: Are mucosal cells from different sources equivalent?

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