Factors controlling high efficiency adventitious bud formation and plant regeneration from in vitro leaf explants of roses (Rosa hybrida L.)

Ibrahim, Rusli; Debergh, Pierre

doi:10.1016/s0304-4238(00)00189-8

Cited by 52 publications

(31 citation statements)

References 26 publications

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“…Treatment with TDZ has resulted in a wide variety of responses during the propagation of cassava (Konan et al, 1997), roses (Ibrahim andDebergh, 2001), andJ. Plant Biol.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, no plantlets survived after treatment at 24~ This positive response under cooler conditions may have resulted from a reduction in hyperhydricity caused by water vapor condensing on top of the soil (see also Ibrahim and Debergh, 2001 ).…”

Section: Plantlet Acclimatizationmentioning

confidence: 99%

“…Changes in temperature, daylength, light intensity, and water availability throughout the year may modify the levels of carbohydrates, proteins, and growth substances in the stock plant, thus affecting the response of the explant in-vitro. *Corresponding author; fax +82-41-550-3440 e-mail hccha@anseo.dankook.ac.kr Morphology during in-vitro organogenesi~ has been described for leaf explants from roses (Ibrahim and Debergh, 2001) and lavender (Dronne et al, 1999), and from the petioles of grape (Martinelli et al, 2001). Those studies showed that calli developed from the cut edges of the leaf, and were compact and yellowish-white with a few green portions.…”

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confidence: 99%

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Plant regeneration and morphology during in-vitro organogenesis fromHeloniopsis orientalis (Thunb.) C. Tanaka

2002

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Heloniopsis orientali$ (ILiliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to produce plants with vigorous growth characteristics via tissue culture. Leaf tissues were cultured on MS basal media supplemented with growth regulators 2,4-D, TDZ, BA, or zeatin. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.1 to 1.0 mg/L 2,4-D or 0.1 to 3.0 mg/I. BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 0.1 mg/L BA. TDZ triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. After the plants were acclimatized for one month at 4~ they were successfully transferred to soil. In addition, we used LM and SEM to investigate shoot morphogenesis at various stages of differentiation.

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“…Treatment with TDZ has resulted in a wide variety of responses during the propagation of cassava (Konan et al, 1997), roses (Ibrahim andDebergh, 2001), andJ. Plant Biol.…”

Section: Discussionmentioning

confidence: 99%

Section: Plantlet Acclimatizationmentioning

confidence: 99%

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confidence: 99%

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Plant regeneration and morphology during in-vitro organogenesis fromHeloniopsis orientalis (Thunb.) C. Tanaka

2002

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“…Therefore, a tissue culture system in roses has been established (Ibrahim and Debergh, 2001;Hameed et al, 2006;Rout et al, 2006;Drefahl et al, 2007;Previati et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Callogenesis and production of anthocyanin and chlorophyll in callus cultures of vegetative and floral explants in Rosa gallica and Rosa hybrida (Rosaceae)

Tarrahi¹,

Rezanejad²

2013

Turk J Bot

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Callogenesis using vegetative (leaf, stem, petiole) and floral (petal, pistil, anther) explants of Rosa gallica and R. hybrida was investigated using different combinations of 2,4-dichlorophenoxyacetic acid, 6-benzylaminopurine, and gibberellic acid on modified Murashige and Skoog medium. The highest callogenesis was obtained on Murashige and Skoog medium containing ratios of 2 and 3 mg L -1 of 2,4-dichlorophenoxyacetic acid to 1 mg L -1 of 6-benzylamino purine in both species depending on type of explant. Stem explants in R. gallica initiated callus after 4 days while the other explants in R. gallica and all explants in R. hybrida showed the greatest percentage of callus initiation after 8 days. The callus growth rate showed much more progressive callus growth respectively on leaf and stem explants in both species. The highest callus volume was achieved after 2 months from vegetative explants and after 2.5 months from flower explants in both species. The highest anthocyanin and chlorophyll yield was produced in vegetative calluses of R. gallica but their content was less in flower calluses. In R. hybrida, the highest value of pigment was observed in calluses from leaf and stem. It is noteworthy that anthocyanin content in different calluses, especially vegetative ones in R. gallica, was much higher, while chlorophyll concentration was somewhat more in calluses derived from explants of R. hybrida.

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“…Tissue culture systems in roses have already been established [4][5][6][7][8][9][10][11][12] . To establish a flowering research system in vitro, it is necessary to develop a reliable and rapid shoot organogenesis protocol.…”

Section: Introductionmentioning

confidence: 99%

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Sakpeth¹,

Kanchanapoom²

2010

ScienceAsia

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Factors controlling high efficiency adventitious bud formation and plant regeneration from in vitro leaf explants of roses (Rosa hybrida L.)

Cited by 52 publications

References 26 publications

Plant regeneration and morphology during in-vitro organogenesis fromHeloniopsis orientalis (Thunb.) C. Tanaka

Plant regeneration and morphology during in-vitro organogenesis fromHeloniopsis orientalis (Thunb.) C. Tanaka

Callogenesis and production of anthocyanin and chlorophyll in callus cultures of vegetative and floral explants in Rosa gallica and Rosa hybrida (Rosaceae)

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