Factors associated with high-risk HPV positivity in a low-resource setting in sub-Saharan Africa

Mitchell, Sheona; Sekikubo, Musa; Biryabarema, Christine; Byamugisha, Josaphat; Steinberg, Malcolm; Jerónimo, José; Money, Deborah; Christilaw, Jan; Ogilvie, Gina

doi:10.1016/j.ajog.2013.08.038

Cited by 31 publications

(28 citation statements)

References 23 publications

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“…The simplicity of the assay and its relative robustness in the context of a resource-constrained laboratory setting was confirmed in this study. Various studies have been conducted using this assay including in Uganda [12], rural China [18], rural Thailand [19] with good outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…This test takes 2.5 to 3 h to perform for a 96 well and involves 6 easy-to-follow steps of denaturation, hybridization and capturing, conjugation, washing, additions of substrate and detection with the illuminometer. The results obtained are qualitative for hr-HPV without indicating the specific genotype [7, 12, 13]. In order to verify testing reproducibility, a random 50% of samples were retested without knowledge of prior result.…”

Section: Methodsmentioning

confidence: 99%

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Options in human papillomavirus (HPV) detection for cervical cancer screening: comparison between full genotyping and a rapid qualitative HPV-DNA assay in Ghana

Obiri‐Yeboah

Adu‐Sarkodie

Djigma

et al. 2017

gynaecol oncol res pract

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BackgroundModern cervical cancer screening increasingly relies on the use of molecular techniques detecting high-risk oncogenic human papillomavirus (hr-HPV). A major challenge for developing countries like Ghana has been the unavailability and costs of HPV DNA-based testing. This study compares the performance of careHPV, a semi-rapid and affordable qualitative detection assay for 14 hr-HPV genotypes, with HPV genotyping, for the detection of cytological cervical squamous intraepithelial lesions (SIL).MethodsA study comparing between frequency matched HIV-1 seropositive and HIV-seronegative women was conducted in the Cape Coast Teaching Hospital, Ghana. A systematic sampling method was used to select women attending clinics in the hospital. Cervical samples were tested for HPV by careHPV and Anyplex-II HPV28 genotyping assay, and by conventional cytology.ResultsA total of 175 paired results (94 from HIV-1 seropositive and 81 from HIV-seronegative women) were analyzed based on the ability of both tests to detect the 14 hr-HPV types included in the careHPV assay. The inter-assay concordance was 94.3% (95%CI: 89.7–97.2%, kappa = 0.88), similar by HIV serostatus. The careHPV assay was equally sensitive among HIV-1 seropositive and seronegative women (97.3% vs. 95.7%, p = 0.50) and slightly more specific among HIV-seronegative women (85.0% vs. 93.1%, p = 0.10). careHPV had good sensitivity (87.5%) but low specificity (52.1%) for the detection of low SIL or greater lesions, but its performance was superior to genotyping (87.5 and 38.8%, respectively). Reproducibility of careHPV, tested on 97 samples by the same individual was 82.5% (95%CI: 73.4–89.4%).ConclusionsThe performance characteristics of careHPV compared to genotyping suggest that this simpler and cheaper HPV detection assay could offer a suitable alternative for HPV screening in Ghana.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Options in human papillomavirus (HPV) detection for cervical cancer screening: comparison between full genotyping and a rapid qualitative HPV-DNA assay in Ghana

Obiri‐Yeboah

Adu‐Sarkodie

Djigma

et al. 2017

gynaecol oncol res pract

View full text Add to dashboard Cite

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“…[17][18][19][20][21][22] Taken together, these results strongly support the use of hrHPV-RNA testing of self-collected specimens as a potential scalable primary screening approach in resource-constrained regions of low-income countries where access to physicians and cytopathologists is limited. These data support the use of hrHPV-RNA testing as a primary screening strategy in resource-limited regions where cytology is largely not available for screening, as long as women with hrHPV-RNA positive results can be assured of appropriate follow-up and treatment.…”

Section: Discussionmentioning

confidence: 62%

High‐risk HPV‐RNA screening of physician‐ and self‐collected specimens for detection of cervical lesions among female sex workers in Nairobi, Kenya

Senkomago

Ting

Kwatampora

et al. 2018

Intl J Gynecology & Obste

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“…Rural residential area reflects poor socio-economic condition possibly related to lack of access to proper care which facilitates infection and persistence of HPV and an increasing risk of cancer development [41]. In rural Odisha where sexual conception and behavior of women are conservative, men possessing multiple sexual partners might be a major source of HPV infection in a female.…”

Section: Discussionmentioning

confidence: 99%

HPV Genotypes distribution in Indian women with and without cervical carcinoma: Implication for HPV vaccination program in Odisha, Eastern India

et al. 2017

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BackgroundConsidering the limited cross protection offered by the current HPV vaccines, understanding the HPV genotype distribution among the different population is essential in predicting the efficacy of current vaccine and devising new vaccine strategy. The present work aimed at investigating the HPV genotypes distribution among women with and without cervical carcinoma in Odisha, Eastern India.MethodsA total of 607 participants have been enrolled between January 2014 and June 2016. L1-PCR, sequencing, and E6/E7 nested multiplex type- specific PCR were performed for HPV detection and genotyping. Cytological distribution of 440 cases includes invasive cervical carcinoma or ICC (n = 210), inflammatory smear (n = 162), normal cytology (n = 68). Statistical analyses were performed by using SPSS version 20.0 software and MediCal version 14.10.2(7). A p-value of ≤ 0.05 was considered statistically significant.ResultsThe overall prevalence of HPV infection was (359/595) 60.33%. Prevalence of HPV infection was 93.80% (197/210) in invasive cervical cancer (ICC) cases, 54.32% (88/162) in inflammatory smear and 19.11% (13/68) in normal cervical cytology. The most prevalent genotype was HPV16 (87.28%) followed by HPV18 (24.56%) and HPV 51(3.46%). The overall prevalence of single type was 76.58% and highest (78.9%) among ICC cases. The most frequent genotype combination after HPV16 + 18(9.4%) was HPV16 + 66 + 68(2.7%) which was frequently observed in inflammatory cytology. Age > 45years, parity ≥3, low socio-economic condition, rural residential area and post menopause state were significantly associated with HPV infection. Multiple infections did not have a significant association with any of the clinicopathological variables (stage, LN metastasis, cell type) except tumor size ≥ 2cm in ICC cases. The impact of 2v, 4v, and 9v vaccines in preventing cervical cancer in Odisha were 89.99, 91.65, and 92.16% respectively.ConclusionThis data would help planning an appropriate strategy for disease monitoring and provides baseline data for post-vaccination surveillance in the region. The nonavalent vaccine would be significant in preventing cervical carcinoma in Odisha. Hence an effective vaccination program based on regional HPV epidemiological profile along with the cervical cancer screening is necessary to reduce the cervical cancer burden in India.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-016-2136-4) contains supplementary material, which is available to authorized users.

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Factors associated with high-risk HPV positivity in a low-resource setting in sub-Saharan Africa

Cited by 31 publications

References 23 publications

Options in human papillomavirus (HPV) detection for cervical cancer screening: comparison between full genotyping and a rapid qualitative HPV-DNA assay in Ghana

Options in human papillomavirus (HPV) detection for cervical cancer screening: comparison between full genotyping and a rapid qualitative HPV-DNA assay in Ghana

High‐risk HPV‐RNA screening of physician‐ and self‐collected specimens for detection of cervical lesions among female sex workers in Nairobi, Kenya

HPV Genotypes distribution in Indian women with and without cervical carcinoma: Implication for HPV vaccination program in Odisha, Eastern India

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