Factors affecting the survival of sheep embryos after transfer within a MOET program

Bari, Farida Yeasmin; Khalid, M.; Haresign, W.; Murray, A.; Merrell, B. G.

doi:10.1016/s0093-691x(02)01162-7

Cited by 43 publications

(63 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the P4 level was significantly higher in the Chronogest ® CR compared to the Eazibreed™ CIDR ® G group, the percentage of ewes that lambed to first service period was not different between treatment groups. According to Bari et al (2003) fertility was not increased with elevated P4 levels. Also regarding litter size, no significant association was found with the P4 level on Day 14 in this study.…”

Section: Discussionmentioning

confidence: 79%

“…This finding implies that nulliparous ewes either had a higher fertilization rate or less embryo loss when compared to pluriparous ewes. In this context Bari et al (2003) found the highest embryo survival rate in yearling recipients used in a MOET program what they explained by the absence of uterine problems caused by previous pregnancies. The high fertility of nulliparous ewes to first service period also demonstrate that a 6-day progestagen priming is sufficient in these animals when seasonal breeding is performed.…”

Section: Discussionmentioning

confidence: 88%

See 1 more Smart Citation

Comparison of 6-day progestagen treatment with Chronogest® CR and Eazi-breed™ CIDR® G intravaginal inserts for estrus synchronization in cyclic ewes

Fleisch¹,

Werne²,

Heckendorn³

et al. 2012

Small Ruminant Research

View full text Add to dashboard Cite

The aim of this study was to compare the efficacy of two different short-term progestagen treatments for estrus synchronization in cyclic ewes. A total of 292 ewes of 3 flocks (A-C) on different farms were treated for 6 days with Eazi-breed™ CIDR® G (n = 145) or Chronogest® CR (n = 147) vaginal inserts in combination with 0.125 mg Cloprostenol and 300IU eCG at device removal. Blood samples were taken for progesterone (P4) determination at insert application and removal as well as 14 days later. One day after insert removal rams fitted with marking harnesses were joined to ewes for 35 days and marked ewes recorded daily. Lambing data were recorded and fertility to first service period and overall assessed. Results show that 24-96 h after insert removal 98.8% and 89.4% of the synchronized ewes in the flocks B and C were in estrus. Estrus response was not affected by the type of progestagen treatment and was lower (P < 0.05) in nulli-(82.1%) than in pluriparous (97.5%) ewes. Type of progestagen treatment did not affect fertility, but flock and parity influenced the percentage of ewes that lambed as well as the lambing rate. Overall more ewes (P < 0.001) lambed in flock A compared to flocks B and C (93.0 vs. 72.5 and 59.1%). In nulliparous animals the percentage of ewes that lambed was higher to the first service (P < 0.05) period but lower (P < 0.001) to the overall service period compared to pluriparous animals (51.5% vs. 49.3% and 64.7% vs. 84.2%). Lambing rates were higher in flock A than in flocks B and C to first service period and overall (1.3 ± 1.4, 0.9 ± 1.0, and 0.7 ± 1.0, P < 0.01 and 1.9 ± 1.1, 1.3 ± 0.9, and 1.0 ± 1.0, P < 0.001) and in nulliparous overall lower than in pluriparous ewes (1.1 ± 1.1 and 1.6 ± 1.1, P < 0.01). Serum P4 concentrations measured 14 days after insert removal were higher in the Chronogest® CR than in the Eazi-breed™ CIDR® G group (6.8 ± 4.1 ng/mL vs. 5.7 ± 3.3 ng/mL). Ewes of flock A had higher P4 values compared to ewes of flocks B and C (8.3 ± 4.1 ng/mL vs. 4.5 ± 1.5 ng/mL and 3.8 ± 1.7 ng/mL) and nulliparous lower P4 concentrations than pluriparous ewes (4.5 ± 2.2 ng/mL vs. 6.7 ± 4.0 ng/mL). In conclusion, a 6-day treatment with Chronogest® CR and Eazi-breed™ CIDR® G with prostaglandin and eCG at insert removal resulted in high estrus response and similar fertility in cyclic ewes. AbstractThe aim of this study was to compare the efficacy of two different short-term progestagen treatments for estrus synchronization in cyclic ewes. A total of 292 ewes 72.5 and 59.1%). In nulliparous animals the percentage of ewes that lambed was higher to the first service (P<0.05) period but lower (P<0.001) to the overall service period compared to pluriparous animals (51.5 vs. 49.3% and 64.7 vs. 84.2%).Lambing rates were higher in flock A than in flocks B and C to first service period and overall (1.3±1.4, 0.9±1.0, 0.7±1.0, P<0.01 and 1.9±1

show abstract

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 88%

Comparison of 6-day progestagen treatment with Chronogest® CR and Eazi-breed™ CIDR® G intravaginal inserts for estrus synchronization in cyclic ewes

Fleisch¹,

Werne²,

Heckendorn³

et al. 2012

Small Ruminant Research

View full text Add to dashboard Cite

show abstract

“…It is well known that the pregnancy rates could be influenced by the quality and developmental stages of transferred embryos (Sağırkaya and Bağış, 2003;Vajta et al, 1998). Bari et al (2003) compared viability of frozen-thawed sheep embryos collected at 5 th and 6 th days of oestrus. They found that the blastocyst stage embryos were collected at 6 th days had higher viability rates than the morula stage embryos collected at 5 th day.…”

Section: Discussionmentioning

confidence: 99%

“…Research Article 151 Bari et al, 2003;Birler et al, 2010). However, the success of these technologies are largely related to the success of oocyte maturation, sperm DNA integrity, fertilization, embryo culture and cryopreservation of the oocyte and embryos in vitro (Akal and Selçuk, 2013;Boland et al, 2001).…”

Section: Araştırma Makalesimentioning

confidence: 99%

Effect of Different Cooling Rates on Embryo Survivability and Pregnancy Rates in Freezing Sheep Embryos

Öztürk¹,

Demır²,

Arıcı³

et al. 2016

İstanbul Üniv. Vet. Fak. Derg.

View full text Add to dashboard Cite

In the first stage of the study, oocytes obtained from ovaries from abbatoir (n=2990) were incubated in maturation medium for 24 h. The maturated oocytes were left for in vitro fertilization (IVF) for 20 hours. The cleavaged embryos (n=1305) were left for in vitro culture (IVC) for six days. Then morula-blastocyst stage embryos divided randomly in three groups (Grup I: 0.5°C/min, Grup II: 0.8°C/min, Grup III: 1°C/min). Embryos in each group (n=50) were frozen at different cooling rates in 1.5 M ethylene glycol containing freezing medium. As a result, 0.5°C/min cooling rate group was found as the most successful group (P<0.05). In the second stage of the study, in vivo embryos (morula-blastocyst) derived from donor sheep were frozen at 0.5 °C/min. Frozenthawed nineteen embryos were transferred to hormonally pretreated seventeen recipients. At the 60 th day of the transfer, three recipients were diagnosed as pregnant and one of them had twins. One of the three recipients gave birth and two sheeps had early embryonic loss are observed in the later ultrasound scanning. In the study, 0.5°C/min cooling rate in the slow freezing of sheep embryos was found out to be more successful cooling rates and pregnancy following a healthy lambing is achieved. Özet Koyun Embriyolarının Dondurulmasında Farklı Soğutma Hızlarının Embriyoların Canlılığı ve Gebelik Oranları Üzerindeki EtkisiÇalışmanın ilk bölümünde, mezbahadan sağlanan ovaryumlardan kazanılan oositler (n=2990) olgunlaştırma medyumu içerisinde 24 saat süreyle olgunlaştırıldı. Ardından, 20 saat süreyle İn Vitro Fertilizasyona (İVF) bırakıldılar. Yarıklanma gösteren embriyolar (n=1305), Sentetik Ovidukt Fluid (SOF) medyumu içerisine alınarak altı gün süresince İn Vitro Kültüre (İVK) bırakıldılar. İVK sonrası elde edilen morula-blastosist aşamasındaki embriyolar rastlantısal şekilde üç farklı dondurma hızı grubuna eşit olarak ayrıldılar (Grup I: 0,5 °C /dk, Grup II: 0,8 °C /dk, Grup III: 1 °C /dk). Her bir gruptaki embriyolar (n=50), 1,5 M etilen glikol bulunan dondurma medyumu içerisinde farklı soğutma hızlarında donduruldu. Sonuçta 0,5 °C/dk soğutma hızının en başarılı grup olduğu belirlendi (P<0,05). Çalışmanın ikinci bölümünde, verici koyunlardan elde edilen in vivo embriyolar (Morula-Blastosist) çalışmanın birinci bölümünde bulunan en başarılı soğutma hızı (0,5 °C/dk) ile soğutularak donduruldu. Dondurulan 19 adet embriyo hormonel olarak hazırlanmış 17 alıcı koyuna transfer edildi. Transfer sonrası 60. günde yapılan ultrason muayenesinde üç adet koyunda gebeliklere ait embriyonik keseler gözlendi ve bu koyunlardan birinde de ikiz gebelik saptandı. Gebe koyunlardan bir tanesinde doğum gerçekleşti; diğer iki koyunda ise ileriki dönemde yapılan ultrason muayenesinde gebeliklerin sonlandığı gözlendi. Çalışma sonucunda, koyun embriyolarının dondurulması sırasında 0,5°C/dk soğutma hızının en başarılı hız olduğu ve bu yöntemle dondurulan embriyoların transferinden de gebelik elde edilebildiği saptandı.

show abstract

“…Bu amaçla kullanılan yöntemlerden birisi MOET (Çoklu yumurtlatma ve embriyo transferi) yöntemi-dir [2][3][4] . Türkiye'nin kendi koşullarına ve yetiştiricinin taleplerine uygun, Türkiye'de yetişmiş ve seçilmiş boğalardan elde edilecek spermayı kendi üretecek duruma gelebilmesi bunun da en kısa sürede ve ucuz bir şekilde yapıla-…”

Section: Makale Kodu (Article Code): Kvfd-2010-3598unclassified

The Determination of Relationship Between Age and the Quality of Embryo of Donor Cows

Hizli¹,

Ayaşan²,

Gök³

et al. 2009

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

ÖzetÇalışmada donör ineklerde (n=165), donör yaşı ile embriyo kalitesi arasındaki ilişki araştırılmıştır. Araştırmada Siyah Alaca donör inekler yaşlarına göre 3 gruba ayrılmıştır. 1. grup yaşları 2-3 yaş arasında değişen 61 inekten oluşurken, 2. grup yaşları 4-5 yaş arasında değişen 28 inekten, 3. grup ta yaşları x≥6 yaş olan 76 inekten oluşmuştur. Çalışmada donör inek yaşı, transfer edilebilen embriyo sayı ortalaması ile toplam embriyo sayı ortalamasını istatistiki olarak etkilemiş (P<0.05); transfer edilemeyen embriyo sayısı gruplar arasında önemli bulunmamıştır (P>0.05). Çalışmada transfer edilebilen embriyo oranı gruplarda %53.46-63.24 arasında değişim göstermiştir. Denemede donör ineklerde donör yaşı ile transfer edilebilen embriyo arasında (r=0.142) yine donör yaşı ile toplam embriyo arasında (r=-0.138) önemli bir ilişki saptanmıştır. Anahtar sözcükler: İnek, Donör yaşı, Embriyo kalitesi The Determination of Relationship Between Age and the Quality of Embryo of Donor Cows SummaryThe relationship between donor age in donor cows (n=165) and quality of embryos was examined in this study. According to their donor age, the Holstein donors were divided into three groups. In the first group there were 61 donors between 2-3 years old; in the second group, 28 donors between 4-5 years old; and in the third group, 76 donors between ≥6 years old. In the study, donor cow age were aff ected transferable embryos and total embryo numbers statistically significant (P<0.05); not transferable embryos in groups were not found statistically significant (P>0.05). The transferable embryo rate in groups was found between 53.46-63.24% in the experiment. A significant correlation (r=0.142) was recorded, i.e. between donor age and transferable embryos; a significant correlation was recorded (r=-0.138), i.e. between donor age and total embryos. Keywords: Cow, Donor age, Embryo quality Donör İneklerde Yaş ile Embriyo Kalitesi Arasındaki İlişkinin SaptanmasıHatice . Türkiye'nin kendi koşullarına ve yetiştiricinin taleplerine uygun, Türkiye'de yetişmiş ve seçilmiş boğalardan elde edilecek spermayı kendi üretecek duruma gelebilmesi bunun da en kısa sürede ve ucuz bir şekilde yapıla- GİRİŞEmbriyo transferi bir dizi biyoteknolojik işlemler dizisidir. Embriyo transferinde hedef, yüksek genotipik ve fenotipik kapasiteye sahip dişi hayvanlardan (donör) elde edilen yavru miktarını artırarak sürü ıslahında genetik ilerlemenin hızlandırılmasıdır. Bu konuda son yıllarda yapı-lan çalışmalara rağmen, bu tekniğin sonuçları tahmin edilememektedir 1 . Süperovulasyon ve embriyo transfer uygulamalarının birlikte yapılmasına rağmen, bu işlemlerin İleti şim (Correspondence) +90 322 388 45 00/24 tugay_ayasan@yahoo.com

show abstract

Factors affecting the survival of sheep embryos after transfer within a MOET program

Cited by 43 publications

References 34 publications

Comparison of 6-day progestagen treatment with Chronogest® CR and Eazi-breed™ CIDR® G intravaginal inserts for estrus synchronization in cyclic ewes

Comparison of 6-day progestagen treatment with Chronogest® CR and Eazi-breed™ CIDR® G intravaginal inserts for estrus synchronization in cyclic ewes

Effect of Different Cooling Rates on Embryo Survivability and Pregnancy Rates in Freezing Sheep Embryos

The Determination of Relationship Between Age and the Quality of Embryo of Donor Cows

Contact Info

Product

Resources

About