Factors affecting the survivability of bovine oocytes vitrified in droplets

Papis, K.; Shimizu, Manabu; Izaike, Yoshiaki

doi:10.1016/s0093-691x(00)00380-0

Cited by 170 publications

(101 citation statements)

References 18 publications

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“…The postwarm survival rates of IVP bovine embryos at Day-3 through Day-7 in the OPS were as high as those of fresh control embryos, whereas post-warm survival r a t e s o f D a y -1 a n d D a y -2 e m b r y o s w e r e significantly impaired [23]. Another ultra-rapid vitrification methods were also reported for bovine oocytes or early-stage embryos, referred to as the minimum drop-size technique [24], micro-droplet m eth od [25], and cr yo loop techn iqu e [26]. However, it was difficult to control precisely the v ol um e of v i t r if i c at i on s olu t i on , p os s ib l y explaining the poor reproducibility of these ultrarapid vitrification procedures.…”

Section: Ultra-rapid Vitrification Using Gl-tip As Containermentioning

confidence: 98%

Cryopreservation and Sexing of In Vivo- and In Vitro-Produced Bovine Embryos for Their Practical Use

Tominaga

2004

Journal of Reproduction and Development

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Abstract. My research awarded includes contributions to cryopreservation and sexing of bovine embryos produced in vitro and in vivo, as follows; (1) In vivo-derived morulae and blastocysts were cryopreserved in the presence of 10% glycerol, and the embryos were transferred into recipients after two-step dilution of glycerol in straw, with a practically acceptable pregnancy rate. (2) The survival rate of 16-cell stage embryos frozen in the medium with ethylene glycol was higher than that with DMSO or 1,2-propanediol. Addition of linoleic acid-albumin to culture medium enhanced the survival rate of post-thaw bovine 16-cell stage in vitro-produced (IVP) embryos. (3) Polarization of cytoplasmic lipid droplets by centrifugation of 2-cell stage embryos was found effective to increase freezing tolerance in 16-cell stage embryos developed from the centrifuged embryos, because blastomeres of 16-cell stage embryos were mostly lipid-free. (4) The usefulness of gel-loading tip (GLTip) as a container for ultra-rapid vitrification was demonstrated in IVP embryos from 2-cell to blastocyst stages, with a higher in vitro survival than the conventional two-step freezing. (5) PCR analysis for sexing of in vivo-derived Day-7 embryos indicated that male embryos developed faster and graded higher than female embryos. But such correlation between genetic sex and embryonic development was not found in IVP embryos obtained from individual cows. (6) Addition of 0.1-1.0% deproteinized hemodialysate product from calf blood to culture medium increased the producing efficiency of demi-embryos with good quality. Female embryos rather than male embryos required a longer time to repair after bisection. (7) In vivo-derived bovine embryos after biopsy for sexing by PCR analysis and subsequent vitrification using GL-Tips are available to practical use in the field. (8) Introduction of primer extension preamplification-PCR and purification of DNA product before standard sexing PCR of biopsy samples from Day 3-4 in vitro-derived embryos allowed accurate sex determination, and Day-7 blastocysts developed from Day 3-4 embryos were cryopreserved by GLTip vitrification without a loss of their viability. Thus the field application of bovine embryo transfer is in part supported by improvements of technologies in embryo cryopreservation and sex predetermination.

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Section: Ultra-rapid Vitrification Using Gl-tip As Containermentioning

confidence: 98%

Cryopreservation and Sexing of In Vivo- and In Vitro-Produced Bovine Embryos for Their Practical Use

Tominaga

2004

Journal of Reproduction and Development

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“…Vitrification can be achieved with short exposure to high concentrations of cryoprotectants followed by very fast cooling and warming rates [5] in order to avoid crystallisation damage. Using standard freezing straws and cryovials or even open-pulled straws [6], actual vitrification of the medium containing the oocytes is not always successful due to the delay of heat loss from the medium [15]. This causes crystallisation, which is detrimental to the oocyte.…”

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confidence: 99%

Cryopreservation of bovine oocytes: is cryoloop vitrification the future to preserving the female gamete?

Mavrides

Morroll

2002

Reprod. Nutr. Dev.

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“…This method was first proposed by Landa and Tepla (1990) for mouse embryos. It was then successfully used for bovine embryos (Riha et al, 1991), zygotes (Yang and Leibo, 1999), both mature oocytes (Papis et al, 2000) and immature oocytes (Kim et al, 2007), and pig embryos (Misumi et al, 2003). However, no further application of this technology has been published, probably because of the difficulties encountered when handling the embryos.…”

Section: New Trends In Vitrification Of Oocytes and Embryosmentioning

confidence: 99%

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

Gajda¹,

Smorąg²

2009

J. Anim. Feed Sci.

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During the past few decades, significant progress in cryopreservation of mammalian oocytes and embryos has been observed. Transfer of cryopreserved embryos or oocytes resulted in live offspring in at least 25 species. So far, two major methods have been used for oocyte and embryo cryopreservation: conventional slow-rate freezing and vitrification. This review summarizes the progress in cryopreservation of mammalian oocytes and embryos that has been achieved by modifying oocyte/embryo susceptibility to cryopreservation or vitrification technology through such techniques as solid-surface vitrification, cryoloop, microdrop, cryotop, electron microscopy grids, nylon mesh, open pulled straw method or removal of lipids, addition of antifreeze protein or cytoskeletonstabilizing agents, cholesterol or liposomes, centrifugation prior to cryopreservation or application of high hydrostatic pressure. In conclusion, the vitrification method opened new perspectives in cryopreservation of embryos and oocytes, both for in vitro fertilized and somatic nuclear transfer. Authors believe that new cryopreservation procedures which modify the susceptibility of oocytes and embryos to cryopreservation will gain importance as a major tool in mammalian gamete and embryo cryopreservation.

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Factors affecting the survivability of bovine oocytes vitrified in droplets

Cited by 170 publications

References 18 publications

Cryopreservation and Sexing of <i>In Vivo</i>- and <i>In Vitro</i>-Produced Bovine Embryos for Their Practical Use

Cryopreservation and Sexing of <i>In Vivo</i>- and <i>In Vitro</i>-Produced Bovine Embryos for Their Practical Use

Cryopreservation of bovine oocytes: is cryoloop vitrification the future to preserving the female gamete?

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

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