Cryopreservation of bovine oocytes: is cryoloop vitrification the future to preserving
the female gamete?

Mavrides, Andreas; Morroll, David

doi:10.1051/rnd:20020008

Cited by 42 publications

(30 citation statements)

References 20 publications

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“…The cooling rate is decided mainly by the structure of vitrification carriers and the manipulation procedures. At present, an increased cooling rate is achieved mostly by plunging small-volume samples held on tiny vitrification carriers, such as cryoleaf, cryoloop, and ministraw [10][11][12][13], directly into liquid nitrogen (LN 2 ). Therefore, conventional vitrification methods have hardly ever been considered for the cryopreservation of a large quantity of vigorously proliferating HES cell lines.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Human Embryonic Stem Cells with a New Bulk Vitrification Method1

Mai

Gao

et al. 2010

Biology of Reproduction

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To increase the manipulation efficiency and storage capability of vitrified human embryonic stem cells, a new bulk vitrification method was established using transformed cryovials. This method vitrified a large number of cell clumps, as opposed to those cryopreserved by a slow-freezing method with conventional cryovials at one time (round). After warming, vitrified human embryonic stem cells exhibited a much higher survival rate than the slow-freezing cells. The vitrified stem cells continued to express markers of pluripotency and formed teratomas in mice with severe combined immunodeficiency, confirming the pluripotency of vitrified-warmed human embryonic stem cell clumps. The new bulk vitrification method is superior to and more practical than the open pulled straw vitrification method and the slow-freezing method for the cryopreservation of human embryonic stem cells.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation of Human Embryonic Stem Cells with a New Bulk Vitrification Method1

Mai

Gao

et al. 2010

Biology of Reproduction

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“…These new high speed vitrification methods dramatically decrease the chilling injury, permit the use of less concentrated and less toxic solutions and shorten the time of exposure with the final cryoprotectant both before cooling and after warming. High rates of survival have been obtained using a new cryoloop vitrification technique followed by ICSI [44]. Additionally, the small volume of the solution prevents heterogeneous ice formation [45].…”

Section: How To Improve Oocyte Cryopreservation?mentioning

confidence: 99%

“…Consequently, a considerable advance in the cryopreservation of certain mammalian ova and embryos has been achieved. These results have generated a search for new methods for further increasing the cooling rates [43,44].…”

Section: How To Improve Oocyte Cryopreservation?mentioning

confidence: 99%

Cryopreservation of bovine oocytes: Current status and recent developments

Massip

2003

Reprod. Nutr. Dev.

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-The cryopreservation of oocytes of most animal species remains a challenge due to their complex structure. Equilibrium freezing is not satisfactory because oocytes seem to be damaged by exposure for several minutes to temperatures near 0 ºC. Therefore, cryopreservation of oocytes by vitrification -especially the use of very high cooling rates of oocytes suspended in extremely small volumes of various cryoprotective additive modifications -seems the most appropriate method. oocyte / bovine / cryopreservation

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“…Le dispositif est plongé immédiatement dans l'azote liquide contenu dans le cryotube lui-même immergé. Cette technique a permis de vitrifier avec succès des ovocytes et des embryons de plusieurs espèces telles que la souris [13,15], le hamster [14], la vache [14,16] et l'Homme [15] ; • la spatule dans laquelle les embryons sont déposés avec un volume de milieu de vitrification inférieur à 1 μl. Cette spatule est ensuite directement plongée dans l'azote liquide et stockée telle quelle ou conditionnée dans une paillette partiellement immergée dans l'azote après vitrification [17,18].…”

Section: Introductionunclassified