Factors affecting the structure and maturation of human tissue engineered skeletal muscle

Martin, Neil R.W.; Passey, Samantha; Player, Darren J.; Khodabukus, Alastair; Ferguson, Richard A.; Sharples, Adam P.; Mudera, Vivek; Baar, Keith; Lewis, Mark P.

doi:10.1016/j.biomaterials.2013.04.002

Cited by 69 publications

(80 citation statements)

References 34 publications

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“…These have been described in vitro both in 2D 29 as well as in 3D muscle cultures. 1,30 We performed confocal microscopy at day 7 of cocultures, whereas crossstriations were reported only after 14 days of culture. Next to a prolonged culture time, supplementing EGM-2 with specific growth factors to stimulate myoblast fusion, such as MAGIC-F1 protein, IGF-1, and follistatin, 31 can be explored in the future.…”

Section: % (F) Huvecsmentioning

confidence: 99%

Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle

Gholobova

Decroix

Muylder

et al. 2015

Tissue Engineering Part A

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Section: % (F) Huvecsmentioning

confidence: 99%

Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle

Gholobova

Decroix

Muylder

et al. 2015

Tissue Engineering Part A

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“…6,7 Following sufficient recovery time, engineered skeletal muscles implanted into an in vivo regenerative environment have advanced toward the adult phenotype, 8,9 and several studies have attempted to utilize key chemical and mechanical stimuli to improve the maturity of these engineered muscles in vitro. 10,11 Tissue engineers have used the influence of such growth factors on myogenesis to direct techniques for engineering skeletal muscle. Current tissue engineering techniques utilize scaffold materials, ranging from acellularized tissues 12,13 to collagen and fibrin hydrogels, 3,6,14 or opt for a scaffold-free approach 7,9,15 to support development of extracellular matrix (ECM) for subsequent muscle tissue.…”

mentioning

confidence: 99%

Effects of Dexamethasone on Satellite Cells and Tissue Engineered Skeletal Muscle Units

Syverud

VanDusen

Larkin

2016

Tissue Engineering Part A

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Tissue engineered skeletal muscle has potential for application as a graft source for repairing soft tissue injuries, a model for testing pharmaceuticals, and a biomechanical actuator system for soft robots. However, engineered muscle to date has not produced forces comparable to native muscle, limiting its potential for repair and for use as an in vitro model for pharmaceutical testing. In this study, we examined the trophic effects of dexamethasone (DEX), a glucocorticoid that stimulates myoblast differentiation and fusion into myotubes, on our tissue engineered three-dimensional skeletal muscle units (SMUs). Using our established SMU fabrication protocol, muscle isolates were cultured with three experimental DEX concentrations (5, 10, and 25 nM) and compared to untreated controls. Following seeding onto a laminin-coated Sylgard substrate, the administration of DEX was initiated on day 0 or day 6 in growth medium or on day 9 after the switch to differentiation medium and was sustained until the completion of SMU fabrication. During this process, total cell proliferation was measured with a BrdU assay, and myogenesis and structural advancement of muscle cells were observed through immunostaining for MyoD, myogenin, desmin, and a-actinin. After SMU formation, isometric tetanic force production was measured to quantify function. The histological and functional assessment of the SMU showed that the administration of 10 nM DEX beginning on either day 0 or day 6 yielded optimal SMUs. These optimized SMUs exhibited formation of advanced sarcomeric structure and significant increases in myotube diameter and myotube fusion index, compared with untreated controls. Additionally, the optimized SMUs matured functionally, as indicated by a fivefold rise in force production. In conclusion, we have demonstrated that the addition of DEX to our process of engineering skeletal muscle tissue improves myogenesis, advances muscle structure, and increases force production in the resulting SMUs.

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“…The first studies of engineered human tissue demonstrated that the resulting tissues displayed similar morphology to muscles engineered from other species [Chiron et al, 2012;Martin et al, 2013] and progressively increase passive force and viscoelastic properties following mechanical intervention [Powell et al, 2002]. Active tension has been measured in fibrin-Matrigel hydrogels [Madden et al, 2015] and in 2D cultures where individual myotube function can be assayed using a modified atomic force microscopy technique [Smith et al, 2014].…”

Section: Human Cellsmentioning

confidence: 99%

Factors That Affect Tissue-Engineered Skeletal Muscle Function and Physiology

Khodabukus

Baar

2016

Cells Tissues Organs

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Tissue-engineered skeletal muscle has the promise to be a tool for studying physiology, screening muscle-active drugs, and clinical replacement of damaged muscle. To maximize the potential benefits of engineered muscle, it is important to understand the factors required for tissue formation and how these affect muscle function. In this review, we evaluate how biomaterials, cell source, media components, and bioreactor interventions impact muscle function and phenotype.

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Factors affecting the structure and maturation of human tissue engineered skeletal muscle

Cited by 69 publications

References 34 publications

Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle

Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle

Effects of Dexamethasone on Satellite Cells and Tissue Engineered Skeletal Muscle Units

Factors That Affect Tissue-Engineered Skeletal Muscle Function and Physiology

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