Factors That Affect Tissue-Engineered Skeletal Muscle Function and Physiology

Khodabukus, Alastair; Baar, Keith

doi:10.1159/000446067

Cited by 24 publications

(27 citation statements)

References 83 publications

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“…These results suggest that the crosslinking density of hydrogels depends on the composite material, UV dosage, and type of photoinitiator. Furthermore, as previously indicated, the mechanical properties of hydrogels strongly impact muscle function and phenotype . Therefore, it is of great interest to make hydrogels mimicking the biological scaffolds with tunable mechanical properties.…”

Section: Resultsmentioning

confidence: 95%

“…Furthermore, as previously indicated, the mechanical properties of hydrogels strongly impact muscle function and phenotype. [39,[42][43][44]52] Therefore, it is of great interest to make hydrogels mimicking the biological scaffolds with tunable mechanical properties. It is possible to increase the mechanical properties of hydrogels to increase the hydrogel concentration, the UV dosage, or the photoinitiator.…”

Section: Composite Hydrogels With Tunable Mechanical Propertiesmentioning

confidence: 99%

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Composite Biomaterials as Long‐Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue

García-Lizarribar

Fernández-Garibay

Velasco-Mallorquí

et al. 2018

Macromolecular Bioscience

116

134

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New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle. Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride. However, it is difficult to obtain a single hydrogel that meets all the desirable properties for tissue engineering. In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures. In this work, a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds are presented. Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends. Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA. All these composites show high resistance to degradation maintaining the 3D structure with high fidelity over several weeks. Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.

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Section: Resultsmentioning

confidence: 95%

Section: Composite Hydrogels With Tunable Mechanical Propertiesmentioning

confidence: 99%

Composite Biomaterials as Long‐Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue

García-Lizarribar

Fernández-Garibay

Velasco-Mallorquí

et al. 2018

Macromolecular Bioscience

116

134

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“…[9,116,126,141,151] Notably, even when similar tissue-engineering methodologies are used, specific forces vary widely depending on the source of myogenic cells, increasing from C2C12, to human, to adult rat, to neonatal rat cells. [152] Engineered muscles also retain the contractile and metabolic phenotype specific to the muscle from which cells are isolated demonstrating that epigenetic differences in vivo are retained in vitro. [153,154] The ability to engineer muscle with a particular fiber type is important for studying certain diseases such as Pompe disease, Duchenne muscular dystrophy (DMD), and sarcopenia that preferentially target fast muscle fibers.…”

Section: Methods To Improve Engineered Muscle Functionmentioning

confidence: 99%

In Vitro Tissue‐Engineered Skeletal Muscle Models for Studying Muscle Physiology and Disease

Prabhu

Wang

Bursac

2018

Adv Healthcare Materials

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Healthy skeletal muscle possesses the extraordinary ability to regenerate in response to small-scale injuries; however, this self-repair capacity becomes overwhelmed with aging, genetic myopathies, and large muscle loss. The failure of small animal models to accurately replicate human muscle disease, injury and to predict clinically-relevant drug responses has driven the development of high fidelity in vitro skeletal muscle models. Herein, the progress made and challenges ahead in engineering biomimetic human skeletal muscle tissues that can recapitulate muscle development, genetic diseases, regeneration, and drug response is discussed. Bioengineering approaches used to improve engineered muscle structure and function as well as the functionality of satellite cells to allow modeling muscle regeneration in vitro are also highlighted. Next, a historical overview on the generation of skeletal muscle cells and tissues from human pluripotent stem cells, and a discussion on the potential of these approaches to model and treat genetic diseases such as Duchenne muscular dystrophy, is provided. Finally, the need to integrate multiorgan microphysiological systems to generate improved drug discovery technologies with the potential to complement or supersede current preclinical animal models of muscle disease is described.

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“…Stiffness gradients of hydrogel or polyacrylamide and stretch bioreactors are also used to study the effects of mechanotransduction, as shown for MSC migration and fate; and adipogenesis was significantly upregulated by gels that mimicked the native stiffness of adipose tissue (2 kPa) . For contractile tissues such as skeletal muscles, mechanical forces and structural strength are of particular importance (as well as electrical stimulation) and there is much interest in such bioengineered muscle constructs …”

Section: Part A: Major Advances In Tissue Culture For Bioengineering:mentioning

confidence: 99%

Obstacles and challenges for tissue engineering and regenerative medicine: Australian nuances

Grounds

2018

Clin Exp Pharma Physio

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The clinical need and historical approaches to tissue engineering as applied to regenerative medicine are introduced, along with comments on activities in this field around Australia, and then the huge advances for tissue culture studies are discussed (Part A). Combinations of human stem cells and new approaches for generating bioscaffolds present great opportunities for in vitro studies of basic biology and physiology, drug testing and high throughput screening for the pharmaceutical industry, and the advanced tissue engineering of organs and devices. The future here is bright. The major obstacles arise with in vivo application of these bioengineering advances using animal models and humans (Part B), and the complexity of living tissues and the challenges of increased scale required for clinical translation to the large human situation are first discussed. While clinical success seen with implantation of acellular bioscaffolds (with population by host cells) is likely to expand for human use, the major challenge relates to (generally) low survival in vivo of (donor or autologous) cells that are expanded and grown in tissue culture before implantation into the living body. Another major challenge is revascularisation of implanted tissues/organs at the human scale. The innovative approaches and rapid advances in tissue bioengineering hold great promise for overcoming these major obstacles and extending the clinical applications of these technologies.

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Factors That Affect Tissue-Engineered Skeletal Muscle Function and Physiology

Cited by 24 publications

References 83 publications

Composite Biomaterials as Long‐Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue

Composite Biomaterials as Long‐Lasting Scaffolds for 3D Bioprinting of Highly Aligned Muscle Tissue

In Vitro Tissue‐Engineered Skeletal Muscle Models for Studying Muscle Physiology and Disease

Obstacles and challenges for tissue engineering and regenerative medicine: Australian nuances

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