Factors affecting the size distribution of liposomes produced by freeze–thaw extrusion

Optimization of RGD-modified Nano-liposomes Encapsulating Eptifibatide

“…In the liquid nitrogen, ice crystals are formed across lipid bilayers (23,24), and cause lipid fragmentation during the freezing process. During the thawing step, water channels and pores are formed as a result of ice crystals melting.…”

Liposomes/Chitosan Scaffold/Human Fibrin Gel Composite Systems for Delivering Hydrophilic Drugs—Release Behaviors of TirofibanIn Vitro

“…The procedures for preparing liposomes have been presented elsewhere (Castile and Taylor 1999;Chung et al 2006). PC (e.g., 0.32 g), cholesterol, and vitamin E were all dissolved in 20 ml of 95% alcohol in a molar ratio of 11: 4: 0.1.…”

“…• C before being put through 3 freeze-and-thaw cycles (Meyenburg et al 2000), (Castile and Taylor 1999). After the above process, the suspensions were dialyzed through a flow-type membrane dialyzer with a cut-off molecular weight of 10 kDa (Spectrum Laboratory, USA) to remove unencapsulated Tirofiban and to concentrate liposome suspensions.…”

Primitive Membrane Formation, Characteristics and Roles in the Emergent Properties of a Protocell

“…(5) (6) (7) (8) The study of vesicles has adopted and invented many different techniques for analysis and comparison [11][12][13][14][15][16][17]. The Critical Vesicle Concentration (CVC), which the minimal concentration of a given amphiphile needed to form vesicles, is particularly important when using single chain amphiphiles as they are characterized by a high CVC due to their reduced hydrophobic interactions compared to double-chained lipids.…”