Factors Affecting the Rate of Coupling of Bilirubin and Conjugated Bilirubin in the van den Bergh Reaction

Bilirubin Uridine Diphospho-glucuronyltransferase in Rat Liver Microsomes: Genetic Variation and Maturation

“…The determination is performed in the presence of nonconjugated bilirubin in media of high ionic strength and variable amounts of protein, solubilizers, and detergents. All of these conditions permit nonconjugated bilirubin to react with the diazo reagent [16]. High blank values have been reported [17,30], and the limitations of this method in the determination of bilirubin glucuronide by direct analysis of liver homogenates have been reviewed [3]: Investigators [11,15] attempting to avoid these limitations have used the reaction with diazotized sulfanilic acid followed by extraction of the formed azo pigments and separation of the conjugated from the nonconjugated azo pigment by column chromatography.…”

The Preparation of Crystalline Bilirubin-C14*

“…Conventional methods for measuring small amounts of bilirubin in biological material, based on the diazo reaction (1-3), have frequently proved unsatisfactory because of relative insensitivity at low pigment concentrations (2,(4)(5)(6), problems encountered in standardization (7)(8)(9), and loss of pigment through attachment to precipitated protein (1)(2)(3). Furthermore, while a small group of tetrapyrrolic chromogens derived from bilirubin can be estimated by their color reaction with p-dimethylaminobenzaldehyde (Ehrlich reagent) (10,11), methods of analysis and quantitation are almost completely lacking for Ehrlich-negative pyrrole compounds (12,13).…”

HYPOPHOSPHATEMIA IN ACUTE & CHRONIC LIVER DISEASES.

“…All tests were done according to the standard universal test methods. [4][5][6][7][8][9][10] Twenty-five patients were recruited into each of these three groups, i.e. patients with acute liver disease, patients with chronic liver disease and control group.…”