Factors affecting the dormancy and germination of bleeding heart [Lamprocapnos spectabilis (L.) Fukuhara] seeds

Abstract

Information on the optimal conditions to promote the germination of Lamprocapnos spectabilis (L.) Fukuhara seeds is limited; consequently, this study was conducted to establish the requirements to break seed dormancy and promote germination.• The selected seeds had morphophysiological dormancy and had not begun embryo development. To study the dormancy breaking and embryo development processes, seeds were subjected to constant or changing temperature treatments during moist stratification.• High temperature and humidity resulted in vigorous embryo growth, with the longest embryos occurring after 1 month of incubation at 20°C. At 4°C, the seeds required incubation period of at least 3 months to germinate. Embryo growth and germination were higher with changing high and low temperatures than under a constant temperature, and changing temperatures also considerably changed the endogenous hormone levels, embryo development and germination. Bioactive gibberellin (GA) content was higher in seeds incubated at 20°C for 1 month, then at 4°C for 2 months. The content of endogenous abscisic acid in seeds subjected to the same treatment decreased by 97.6% compared with that of the untreated seeds.• Embryo growth and seed germination require changing high and low temperatures; however, exogenous GA 3 could substitute for high temperatures, as it also causes accelerated germination. In this study, the seeds of L. spectabilis were identified as an intermediate simple type, a sub-level of morphophysiologically dormant seeds.Plant Biology 22 (2020) 514-521

Seed Dormancy Release and Germination Requirements of Cinnamomum migao, an Endangered and Rare Woody Plant in Southwest China

“…The morphology of the seed embryo is generally spatulate, rudimentary, or linear; in some species, it occupies ≤1% of the seed volume (Jaganathan, 2020;Liu et al, 2020). For example, the Lamprocapnos spectabilis seed endosperm comprises the entire internal tissue of the seed, and the embryo is almost invisible (Cho et al, 2020). Similarly, the Acer yangjuechi seed embryo exhibits poor development and low vigor, which lead to a germination failure under suitable conditions (Chen et al, 2017).…”

Exogenous gibberellin can effectively and rapidly break intermediate physiological dormancy of Amsonia elliptica seeds

“…The most common dormancy in terrestrial seed plants is PD ( Baskin and Baskin, 2014 ), which is usually alleviated by temperature (cold moist stratification, warm moist stratification, after-ripening), hormones, or chemical treatments ( Baskin and Baskin, 2004 ; Baskin and Baskin, 2014 ; Cho et al., 2020 ; Shao et al., 2021 ; Baskin and Baskin, 2022 ). Physiological dormancy is divided into two subclasses: regular and epicotyl.…”

Gold nanoparticles and electromagnetic irradiation in tissue culture systems of bleeding heart: biochemical, physiological, and (cyto)genetic effects

“…Next, the seeds must undergo dormancy through winter to germinate in the spring of the following year. Therefore, there is a long period (almost 1 year) between sowing and germination (Cho et al 2020). Moreover, the generative method of reproduction fails to retain the characteristics of the mother plant and fulfill the demand of the pharmaceutical market.…”