Gold nanoparticles and electromagnetic irradiation in tissue culture systems of bleeding heart: biochemical, physiological, and (cyto)genetic effects

Abstract

This study aimed to analyze the effect of various mutagens on the in vitro development, physiological activity, acclimatization efficiency, and genetic integrity of Lamprocapnos spectabilis ‘Valentine’. Gold nanoparticles (AuNPs), microwaves, and X-rays were used at different doses. The profiles of primary and secondary metabolites and the enzymatic activity in the produced plants were studied. The usefulness of various genetic markers in the detection of mutations in the species was compared. The genome size of L. spectabilis was estimated for the first time. It was found that the addition of AuNPs into the culture medium had a positive impact on the in vitro development and multiplication of plants. All of the shoots regenerated adventitious roots, but plants subjected to the longest microwave irradiation (3 × 9 s) and the non-treated control had the lowest acclimatization efficiency. Application of mutagens significantly affected the activity and profile of most enzymes and phytochemicals studied, however, the final effect depended on the agent type and dose. Mutations were detected by DAMD, RAPD, and SCoT markers in 7.5% of plants, but not by ISSRs. Phenotype variation in leaf shape was found in four plants. The genome size of L. spectabilis was found to be very small; about 1281 Mbp.

Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

“…It was observed earlier that green-synthesized biogenic Ca-AuNPs and PEGMA-AuNPs enhanced the fresh weight, dry weight, and total biomass in banana under in vitro conditions [ 33 ]. Furthermore, after 10 weeks of in vitro culture of Lamprocapnos spectabilis , treatment of AuNPs increased the shoot number, but reduced rooting [ 34 ]. Moreover, 10 mg/L of citrate-AuNPs were previously shown to influence all of the growth parameters of Arabidopsis [ 35 ].…”

Effect of Silver Nanoparticles on the In Vitro Regeneration, Biochemical, Genetic, and Phenotype Variation in Adventitious Shoots Produced from Leaf Explants in Chrysanthemum

“…Our findings confirm the genotoxic character of nanoparticles, also described by other authors [41], particularly if applied at a high concentration. It can also be suggested that AgNPs have a higher mutagenic potential than the gold nanoparticles that induced variation only in 7.5% of Lamprocapnos spectabilis (L.) Fukuhara plants at 100 mg•L −1 concentration [42].…”

Nanoparticle-mediated enhancement of plant cryopreservation: Cultivar-specific insights into morphogenesis and biochemical responses in Lamprocapnos spectabilis (L.) Fukuhara ’Gold Heart’ and ’Valentine’

“…Finally, nanosensors can monitor soil conditions, nutrient levels, and crop health in real-time, enabling farmers to make data-driven decisions for optimal crop management [5]. Nanoparticles are also widely used in plant biotechnology and in vitro cultures for the disinfection of explants, as growth stimulators and mutagen agents [6,7].…”

“…On the other hand, their addition into the recovery medium after thawing had a deleterious effect, especially if used at a high concentration (30 ppm). More studies in this regard are needed, including the use of other nanoparticle types and sizes, but at a concentration low enough to minimize their phyto-and genotoxic effects reported previously [6,7,9].…”