Factors Affecting the Development and Hatching of Eggs and the Survival of Infective Larvae of &lt;i&gt;Haemonchus contortus&lt;/i&gt; in Laboratory Condition

Ashad, F.A.; Anisuzzaman, Md.; Begum, Nurjahan; Dey, Anita Rani; Mondal, M. M. H.

doi:10.3329/pa.v22i1-2.16469

Cited by 5 publications

(5 citation statements)

References 10 publications

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“…Sommerville and Murphy (1983) support this observation and interpret as it might be due to coagulation of germinal mass of the eggs due to high acidic pH level of the media. Ashad et al, (2011) andStringfellow (1986) also found that maximum hatching of eggs and development of Trichostrongylus spp. larvae occurred at pH 6.0-7.0 which also support the present observation.…”

Section: In Vitro Culture Of Trichostrongylus Spp In Goatsmentioning

confidence: 99%

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Prevalence and in Vitro Culture of Trichostrongylus Spp. in Goat at Trishal, Mymensingh, Bangladesh

Rajib¹

2014

J. adv. parasitol.

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Trichosrtongylus spp., Looss, 1905 is one of the important nematode of alimentary canal that causes a great harm to the ruminants. The study was undertaken to determine the prevalence of Trichostrongylus spp. and effects of temperature, pH, humidity, light and dark, nutrient and other media on the hatching of eggs, the development and survival of larvae of Trichostrongylus spp. Samples were collected from goats from different villages and slaughter houses of Trishal, Mymensingh, Bangladesh, during the period from January to May, 2014. A total of 82 fecal and 67 visceral samples (abomasum, small intestine and large intestine) were collected. Overall prevalence was 20.73% and 14.93% for fecal samples and visceral samples, respectively. Young (23.64%) were 1.78 times more susceptible to Trichostrongylus infection than adult (14.82%) and which was statistically significant (p < 0.001). It was also revealed that females (23.08%) were 1.31 times more susceptible than male (18.61%) and statistically significant (p < 0.001). Here, we incubated eggs at different temperature gradients, humidity and pH in phosphate buffer saline (PBS), tap water (TP) and normal saline (NS) and PBS with 5%, 10% and 15% serum. Maximum (46.67%) hatching of eggs was observed at 18°C on day 5. and development of eggs arrested at 4°C temperature. No development and hatching occurred at pH 2. Hatching of eggs were better on day 6 in all cases and highest hatching rate observed at pH 6.0 (32.30%). Maximum hatching of eggs (42.86%) was recorded at relative humidity of >80%-90% at day 5. There was no significant effect of light on the development and hatching of eggs of Trichostrongylus spp. Maximum eggs hatched in PBS containing 15% serum (46.67%). Moreover, in this medium, survival rate of larvae was also highest (19.08%). So, it is concluded that PBS containing 15% serum can be used as a suitable media for hatching of eggs and survival of larvae.All copyrights reserved to Nexus® academic publishers

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Section: In Vitro Culture Of Trichostrongylus Spp In Goatsmentioning

confidence: 99%

“…But developments were better in dark than light in all the observed day (Table 3). Islam and Ahmed (1987) and Ashad et al, (2011) also found that dark condition was more effective than light on the hatchability of eggs of Trichostrongylus spp.…”

Section: Dark Condition Is Favorable For Hatching Of Eggsmentioning

confidence: 99%

Prevalence and in Vitro Culture of Trichostrongylus Spp. in Goat at Trishal, Mymensingh, Bangladesh

Rajib¹

2014

J. adv. parasitol.

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“…The primary factors that affect the hatching of freeliving helminth eggs are temperature and humidity [2], though these conditions vary among different parasite species. Other important environmental factors contributing to the hatching and survival of helminths include pH, light, and nutrients [3]. In addition to the effect of environmental and parasitic factors in the hatching process, the host interactions with the parasite or immunity can also impose severe restrictions on this process [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Effect of Host Species on Hatchability of Fasciola hepatica and Fasciola gigantica Eggs from Sheep and Cattle

Larki

Jalali

Goodarzi

et al. 2020

JoMMID

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Successful development of free-living stages of parasitic helminths depends on larva ability to survive, develop, and hatch. In this study, we aimed to study the host role in the hatching process of Fasciola species. Methods: Fasciola hepatica and Fasciola gigantica eggs were collected from adult worms that originated from naturally infected sheep and cattle livers and were incubated at 26±1°C for 15 days. The percentage of hatched and developed eggs were obtained for each isolate under a light microscope. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) was applied to identify the F. hepatica and F. gigantica species. Results: Our findings showed no significant differences in the development rates of F. gigantica and F. hepatica eggs in sheep (69.32% and 72.71%) and cattle (73.56% and74.69%). However, the rates of hatched eggs of F. gigantica and F. hepatica originated from cattle (69.19% and 62.36%) were almost twice the rates in sheep (31.69% and 32.59%), indicating a significant difference. Conclusion:This study demonstrated that host species significantly affect the hatching of Fasciola eggs as the hatching rates of F. gigantica and F. hepatica originated from cattle were higher than those taken from sheep did not affect their larval development. Thus, in addition to environmental factors, the hatching phenomenon is influenced by host species.32. Hofmann AF, Hagey LR, Krasowski MD. Bile salts of vertebrates: structural variation and possible evolutionary significance.

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“…In the environment where the host lives, the favorable temperature and relative humidity have been recognized as the important abiotic factor related to the parasite prevalence. The ideal temperature range for larval development is between 22 and 26°C usually (Ashad et al, 2011), but the Trichuris sp. infecting the alpacas and goats is in higher prevalence in temperature group of 0 < T ≤ 10°C.…”

Section: Discussionmentioning

confidence: 99%

In-vitro Susceptibility of Mycoplasma capricolum Subsp. capripneumoniae Pakistan Strain to Commercially Available Quinolones

Rahman¹,

Khan²,

Sadique³

et al. 2021

PJZ

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Factors Affecting the Development and Hatching of Eggs and the Survival of Infective Larvae of <i>Haemonchus contortus</i> in Laboratory Condition

Cited by 5 publications

References 10 publications

Prevalence and in Vitro Culture of Trichostrongylus Spp. in Goat at Trishal, Mymensingh, Bangladesh

Prevalence and in Vitro Culture of Trichostrongylus Spp. in Goat at Trishal, Mymensingh, Bangladesh

Effect of Host Species on Hatchability of Fasciola hepatica and Fasciola gigantica Eggs from Sheep and Cattle

In-vitro Susceptibility of Mycoplasma capricolum Subsp. capripneumoniae Pakistan Strain to Commercially Available Quinolones

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