Prevalence and in Vitro Culture of Trichostrongylus Spp. in Goat at Trishal, Mymensingh, Bangladesh

Abstract

Trichosrtongylus spp., Looss, 1905 is one of the important nematode of alimentary canal that causes a great harm to the ruminants. The study was undertaken to determine the prevalence of Trichostrongylus spp. and effects of temperature, pH, humidity, light and dark, nutrient and other media on the hatching of eggs, the development and survival of larvae of Trichostrongylus spp. Samples were collected from goats from different villages and slaughter houses of Trishal, Mymensingh, Bangladesh, during the period from January to May, 2014. A total of 82 fecal and 67 visceral samples (abomasum, small intestine and large intestine) were collected. Overall prevalence was 20.73% and 14.93% for fecal samples and visceral samples, respectively. Young (23.64%) were 1.78 times more susceptible to Trichostrongylus infection than adult (14.82%) and which was statistically significant (p < 0.001). It was also revealed that females (23.08%) were 1.31 times more susceptible than male (18.61%) and statistically significant (p < 0.001). Here, we incubated eggs at different temperature gradients, humidity and pH in phosphate buffer saline (PBS), tap water (TP) and normal saline (NS) and PBS with 5%, 10% and 15% serum. Maximum (46.67%) hatching of eggs was observed at 18°C on day 5. and development of eggs arrested at 4°C temperature. No development and hatching occurred at pH 2. Hatching of eggs were better on day 6 in all cases and highest hatching rate observed at pH 6.0 (32.30%). Maximum hatching of eggs (42.86%) was recorded at relative humidity of >80%-90% at day 5. There was no significant effect of light on the development and hatching of eggs of Trichostrongylus spp. Maximum eggs hatched in PBS containing 15% serum (46.67%). Moreover, in this medium, survival rate of larvae was also highest (19.08%). So, it is concluded that PBS containing 15% serum can be used as a suitable media for hatching of eggs and survival of larvae.All copyrights reserved to Nexus® academic publishers

Molecular and phylogenetic characterization of internal transcribed spacer 2 of zoonotic Trichostrongylus species from goats in Bangladesh

“…In the present study, the overall prevalence of Trichostrongylus spp. was 31.45%.The present ndings were higher than the previously reported ndings from different countries of the world like 14.93%in Bangladesh (Rajib et al, 2014) Tan et al, 2014). The exact cause of variations in the prevalence of Trichostrongylus cannot be explained but it may possibly be differences in the geo graphical locations, animal population and their habitats, climatic condition, sample size, and methods of examinations.…”

“…The internal transcribed spacer 2 region of ribosomal RNA gene (ITS2-rDNA) is a useful marker for detecting and analyzing the inter and intraspecies variations and phylogenetic relationships of parasites belongs to the Trichostrongylidae family (Ghasemikhah et al, 2012). Although several studies have been carried out to determine the prevalence, epidemiology and the risk of zoonotic transmission of different Trichostrongylus species throughout the world ( (Rajib, Dey, Begum, Momin, & Talukder, 2014). Therefore, this study was designed to determine the prevalence of different Trichostrongylus species from goat viscera by the morphology and morphometric identi cation and to explore the phylogenetic relationships by amplifying the ITS2 gene using multiplex PCR and sequencing.…”

Perkembangan Telur Cacing Haemonchus contortus Menjadi Larva Stadium Pertama pada Media Air Secara In Situ

“…Berdasarkan hasil penelitian, persentase perkembangan telur cacing H. contortus menjadi L1 secara in situ atau di padang penggembalaan ditampilkan pada Tabel 1., dengan persentase rataan sebesar 26,5%. Hasil penelitian ini lebih tinggi dibandingkan dengan hasil penelitian yang dilakukan oleh Rajib et al (2014), pada cacing Trichostrongylus spp. persentase perkembangan telur dimulai dari hari ke-2 pada pH 6,0 sebesar 12% dan pH 7,0 sebesar 7,98%.…”