Factors Affecting Temporal and Spatial Variations of Microcystins in Gonghu Bay of Lake Taihu, with Potential Risk of Microcystin Contamination to Human Health

Wang, Qing; Niu, Yuan; Xie, Ping; Chen, Jun; Ma, Zhimei; Tao, Min; Qi, Ming; Wu, Laiyan; Li-wei, Guo

doi:10.1100/tsw.2010.172

Cited by 84 publications

(49 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The production of [D-Leu 1 ] MC-LR decreased with increasing temperature, coinciding with the findings of Gorham, 1964;Runnegar et al, 1983;van der Westhuizen and Eloff, 1985;Cood and Poon, 1988;Sivonen, 1990;Rapala et al, 1997;Lehman et al, 2008;Wang et al, 2010 andGiannuzzi et al (2016). van der Westhuizen and Eloff (1985) reported that the optimal growth conditions do not coincide with the production of toxins.…”

Section: Discussionsupporting

confidence: 69%

Mathematical modeling of Microcystis aeruginosa growth and [D-Leu1] microcystin-LR production in culture media at different temperatures

et al. 2017

View full text Add to dashboard Cite

A B S T R A C TThe effect of temperature (26 C, 28 C, 30 C and 35 C) on the growth of native CAAT-3-2005 Microcystis aeruginosa and the production of Chlorophyll-a (Chl-a) and Microcystin-LR (MC-LR) were examined through laboratory studies. Kinetic parameters such as specific growth rate (m), lag phase duration (LPD) and maximum population density (MPD) were determined by fitting the modified Gompertz equation to the M. aeruginosa strain cell count (cells mL À1 ). A 4.8-fold increase in m values and a 10.8-fold decrease in the LPD values were found for M. aeruginosa growth when the temperature changed from 15 C to 35 C. The activation energy of the specific growth rate (Em) and of the adaptation rate (E 1 /LPD) were significantly correlated (R 2 = 0.86). The cardinal temperatures estimated by the modified Ratkowsky model were minimum temperature = 8.58 AE 2.34 C, maximum temperature = 45.04 AE 1.35 C and optimum temperature = 33.39 AE 0.55 C.Maximum MC-LR production decreased 9.5-fold when the temperature was increased from 26 C to 35 C. The maximum production values were obtained at 26 C and the maximum depletion rate of intracellular MC-LR was observed at 30-35 C. The MC-LR cell quota was higher at 26 and 28 C (83 and 80 fg cell À1 , respectively) and the MC-LR Chl-a quota was similar at all the different temperatures (0.5-1.5 fg ng À1 ).The Gompertz equation and dynamic model were found to be the most appropriate approaches to calculate M. aeruginosa growth and production of MC-LR, respectively. Given that toxin production decreased with increasing temperatures but growth increased, this study demonstrates that growth and toxin production processes are uncoupled in M. aeruginosa. These data and models may be useful to predict M. aeruginosa bloom formation in the environment.

show abstract

Section: Discussionsupporting

confidence: 69%

Mathematical modeling of Microcystis aeruginosa growth and [D-Leu1] microcystin-LR production in culture media at different temperatures

et al. 2017

View full text Add to dashboard Cite

show abstract

“…However, up to now, there are no pure isolated cultures of W. naegeliana in the world cyanobacterial collection, and we also failed to maintain any culture after isolation. With respect to Microcystis, and confirming our results on M. botrys, other studies in the field have observed significant correlations between the population abundances and MC concentrations (Wang et al, 2010;Horst et al, 2014, among others).…”

Section: Discussionsupporting

confidence: 91%

Cyanobacterial dynamics and toxins concentrations in Lake Alto Flumendosa, Sardinia, Italy

et al. 2017

View full text Add to dashboard Cite

Seasonal blooms of cyanobacteria (CB) are a typical feature of Lake Alto Flumendosa (Sardinia, Italy). The waters of this lake are used for drinking water supply, for agricultural and industrial uses, and fish farming activities. Since cyanotoxins are not monitored in edible organisms, diet could be a relevant route of human exposure. CB also represent a threat for the health of wild and domestic animals that use lake water for beverage. Therefore, to characterize the CB community and assess the risk for human and animal population, CB dynamic, mcyB+ fraction, and microcystins (MCs) concentration have been followed monthly for 18 months, in three stations. Results confirmed the presence of several toxigenic species. Planktothrix rubescens dominated between August 2011 and April 2012 (3.5×106 cells L-1), alternating with Woronichinia naegeliana (8×106 cells L-1) and Microcystis botrys (9×105 cells L-1). Dolichospermum planctonicum was always present at low densities (104 cells L-1). MCs were detected, at values well below the 1 µg L-1 threshold of WHO for drinking water. The molecular analysis of mcyB gene for P. rubescens indicated the presence of a persistent toxic population (average 0.45 mcyB/16S rDNA). Highly significant linear regressions were found between P. rubescens and the sum of the demethylated MC variants, and between M. botrys and the sum of MC-LR and MC-LA, also when co-occurring, suggesting that these two species were responsible for different MC patterns production. The regression lines indicated a quite stable MC cell quota. However, in some spotted samples very different values were obtained for both MC concentrations and cell quota (from 10-fold lower to 30-40-fold higher than the ‘average’) showing an unexpected significant variability in the rate of toxin production. The relatively low cell densities during the monitoring period is consistent with the low-to absent MC contamination level found in trout muscle; however, the analytical method was affected by low recovery, probably due to MC-protein binding. Our results show that, during the study period, no risk of exposure for the human and animal population occurred. However, the persistence of a complex CB community characterised by a significant toxic fraction suggests the need for periodic monitoring activity. Particularly, the hidden deep summer P. rubescens blooms, located where water is taken for drinking water supply, and M. botrys, able to produce the most toxic MC variants with high cell quota, should be kept under control. The documentation and interpretation of sudden changes in toxins concentrations deserve special attention. This is particularly relevant in proximity of fish farming plants and water catchment sites.

show abstract

“…A range of environmental factors, including nitrogen and phosphorus concentrations (Schindler, 2012;Srivastava et al, 2012;Chaffin and Bridgeman, 2014; Van de Waal et al, 2014), TN : TP ratio (Smith, 1983;Wang et al, 2010b; Van de Waal et al, 2014), temperature (Davis et al, 2009;Rolland et al, 2013), salinity (Tonk et al, 2007), and iron concentration (Ame and Wunderlin, 2005;Nagai et al, 2007;Wang et al, 2010a) have been shown to have pronounced effects on cyanobacterial biomass, cyanobacterial dominance and microcystin production. Nevertheless, the results between studies differ, and there is no clear understanding of the roles of these environmental factors as the triggers of cyanobacterial bloom development and microcystin production.…”

Section: S C Sinang Et Al: Site-specific Environmental Triggers Ofmentioning

confidence: 99%

Local nutrient regimes determine site-specific environmental triggers of cyanobacterial and microcystin variability in urban lakes

Sinang

Reichwaldt

Ghadouani

2015

Hydrol. Earth Syst. Sci.

View full text Add to dashboard Cite

Abstract. Toxic cyanobacterial blooms in urban lakes present serious health hazards to humans and animals and require effective management strategies. Managing such blooms requires a sufficient understanding of the controlling environmental factors. A range of them has been proposed in the literature as potential triggers for cyanobacterial biomass development and cyanotoxin (e.g. microcystin) production in freshwater systems. However, the environmental triggers of cyanobacteria and microcystin variability remain a subject of debate due to contrasting findings. This issue has raised the question of whether the relevance of environmental triggers may depend on site-specific combinations of environmental factors. In this study, we investigated the site-specificity of environmental triggers for cyanobacterial bloom and microcystin dynamics in three urban lakes in Western Australia. Our study suggests that cyanobacterial biomass, cyanobacterial dominance and cyanobacterial microcystin content variability were significantly correlated to phosphorus and iron concentrations. However, the correlations were different between lakes, thus suggesting a sitespecific effect of these environmental factors. The discrepancies in the correlations could be explained by differences in local nutrient concentration. For instance, we found no correlation between cyanobacterial fraction and total phosphorous (TP) in the lake with the highest TP concentration, while correlations were significant and negative in the other two lakes. In addition, our study indicates that the difference of the correlation between total iron (TFe) and the cyanobacterial fraction between lakes might have been a consequence of differences in the cyanobacterial community structure, specifically the presence or absence of nitrogen-fixing species. In conclusion, our study suggests that identification of significant environmental factors under site-specific conditions is an important strategy to enhance successful outcomes in cyanobacterial bloom control measures.

show abstract

Factors Affecting Temporal and Spatial Variations of Microcystins in Gonghu Bay of Lake Taihu, with Potential Risk of Microcystin Contamination to Human Health

Cited by 84 publications

References 46 publications

Mathematical modeling of Microcystis aeruginosa growth and [D-Leu1] microcystin-LR production in culture media at different temperatures

Mathematical modeling of Microcystis aeruginosa growth and [D-Leu1] microcystin-LR production in culture media at different temperatures

Cyanobacterial dynamics and toxins concentrations in Lake Alto Flumendosa, Sardinia, Italy

Local nutrient regimes determine site-specific environmental triggers of cyanobacterial and microcystin variability in urban lakes

Contact Info

Product

Resources

About