The laboratory-scale fiber thread carrier sequencing batch biofilm reactor (SBBR) was developed to treat municipal wastewater with different C//N ratios. The influence of C/N ratios on nitrogen removal was evaluated under different conditions of T temperature =(25±1)℃, V aeration =150 L/h, t operation =15 d, 20 d, 20 d. When the C/N ratios are 10.0, 5.0, 3.3, the average removal rates of COD were 87.2%, 92.3% and 94.3%, those of NH 3 -N were 99.3%, 99.7% and 78.1%, and those of TN were 92.9%, 67.3% and 48.2%, respectively. The C/N ratios had little effect on the NH 3 -N removal, while they had greater effect on the removal of TN. When the C/N ratio was low, the removal efficiency of nitrogen decreased. In the SBBR, simultaneous nitrification and denitrification (SND) occurred throughout the study. Moreover, the level of denitrification creased with the C/N ratio creasing.