Factors affecting in vitro propagation of Momordica balsamina: a medicinal and nutritional climber

Thakur, Gulab S.; Pandey, Mukeshwar; Sharma, Rajnish; Sanodiya, Bhagwan S.; Prasad, Gbks; Bisen, Prakash S.

doi:10.1007/s12298-011-0052-z

Cited by 12 publications

(10 citation statements)

References 14 publications

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“…Less number of shoots produced when explant inoculated in germination media supplemented with 0.01 µM BAP (1.858±0.378). This show 1.0 µM of BAP is enough for stimulation proliferation of shoot in this medicinal plant which is in agreement with Gulab et al (2011). Figure 2c indicates the linear increase in the graph length of shoots from 0.01 µM to 2.0 µM of BAP.…”

Section: Resultssupporting

confidence: 82%

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Anuar¹,

Tan²,

Mahmud³

et al. 2019

American Journal of Biochemistry and Biotechnology

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Medicinal plants which is Clinacantus nutans (C. nutans) that known as Sabah Snake Grass or Belalai Gajah can treat several diseases including cancer. In order to provide and grow this plant in large number for health and pharmaceutical industry, one of the solutions is through the plant tissue culture technique. In this experiment, C. nutans was cultured under controlled conditions which to provide sterile plantlets. The surface sterilization using three methods which involved ethanol, Clorox and mercuric chloride. In the comparison of them, it was found that the use of 70% ethanol (5 min) followed by 30% clorox (5 min) then with 0.1% mercuric chloride for one hour was the most effective sterilization method which gave the highest survival rate (100%) and zero contamination rate. The sterile explants were then cultured in media containing different concentrations of Benzylaminopurine (BAP) and Pleurotus ostreatus Spent Mushroom Compost (SMC). The SMC was tested and compared in powder and extracted form. The observations were made every week on responds of the culture using variables which are number of shoots, length of shoots and number of leaves. This article was aimed to evaluate the enhancement effect of BAP and SMC in micro-propagation of Sabah Snake Grass. The authors found that the best concentration for BAP is 1.0 µM. It produced 3.5±0.926 number of shoots and 2 µM BAP produced 7.286±1.496 number of leaves in their findings. When BAP and SMC were tested in combination, they managed to produce high number of shoots and leaves. Their result revealed a number of shoots (5.750±0.500) produced by 2% extract of SMC and some leaves (11.890±1.965) by 8% extract of SMC when both were cultured together with 1.0 µM BAP respectively. The authors thought that there was enhancement effect on the combination of BAP with extracts of SMC which gave about double regeneration rate as compared with the BAP alone and this method had high potential for future research in producing mass propagation of C. nutan and other related study by reducing the usage of plant growth regulator which may reduce the cost of micro-propagation of tissue culture plants.

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Section: Resultssupporting

confidence: 82%

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Anuar¹,

Tan²,

Mahmud³

et al. 2019

American Journal of Biochemistry and Biotechnology

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“…In [10] also reported the similar observation which BAP was the most effective hormone for plant regeneration compared to other. This shows that BAP have high stimulating effect on bud break and multiple shoot formation [11].…”

Section: Introductionmentioning

confidence: 84%

Evaluation on the effects of P. ostreatus spent mushroom compost and BAP hormone towards C. nutans in vitro culture

Hashim

Anuar

Tay

et al. 2018

J. Fundam and Appl Sci.

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“…Many herbal extracts have been reported for their anti-diabetic activities and are currently being used in Ayurveda for the treatment of diabetes. However, such medicinal plants have not gained much importance as medicines due to the lack of sustained scientific evidence (Thakur et al, 2011).…”

Section: Effect Of Bap+nb 6 On Multiplication Of Shoot From Nodal Expmentioning

confidence: 99%

“…The control of glucose level is important aspect for type 2 diabetes, hence control of starch degradation by enzyme such as α amylase and glycosidase play a key role in diabetes. This enzyme is major digestive enzyme and helps in intestinal absorption (Thakur et al, 2011). The treatment of diabetes is the potential targets in the development of lead compound are alpha amylase and glycosidase inhibitors (Nair et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

In vitro Shoot Multiplication from Nodal Explants of Coccinia grandis (L.) Voigt. and It’s Antidiabetic and Antioxidant Activity

Patel¹,

Ishnava²

2015

Asian J. of Biological Sciences

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Coccinia grandis (L.) Voigt. (Cucurbitaceae) is an important vegetable with high food and medicinal value. In this plant major problem is seed germination, seed setting and less number of fruit settings. Plant tissue culture technology may help to overcome these problems to a great extent. Therefore, the present study has been designed to develop an efficient protocol for in vitro shoot multiplication, as well as to check its antidiabetic and antioxidant activity. The nodal segments used as explants for cultured. Murashige and Skoog's (MS) agar-gelled medium with different concentration and combination of BAP, KIN, NAA and NB 6 for shoots multiplication and callus. Multiple shoots was seen after 10 days of sub culturing on MS medium. Maximum shoot formed (16±1.5) on MS medium containing 0.5 mg LG 1 BAP combination with 0.2 mg LG 1 NB 6 after 15 days. Antidiabetic activity of C. grandis was also evaluated by using α-amylase inhibition assay and DNS assay. Methanolic extract of fruit, callus (BAP+NB 6 ) and different combination mixture of callus at 1.0 mg LG 1 concentration showed 54.29, 75.72 and 80.00% inhibition of α-amylase activity respectively. The result of agar diffusion amylase inhibition assay indicated that methanolic extract of callus show maximum inhibition (46.66%) compare to the fruit extract and fresh callus showed no inhibition. Antioxidant activity of C. grandis was evaluated by using DPPH assay method is based on the reduction of methanolic solution of coloured free radical DPPH by free radical scavenger. The DPPH radical scavenging activity and significantly decreased the formation of oxygen radicals generated in rat peritoneal macrophages plant extracts. The protocol described here is efficient with high number of shoot multiplication and can be employed for the large scale production and genetic manipulation for antidiabetic medicinal plant Coccinia grandis.

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Factors affecting in vitro propagation of Momordica balsamina: a medicinal and nutritional climber

Cited by 12 publications

References 14 publications

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Enhancement Effect of BAP and Spent Mushroom Compost in Micro-Propagation of Sabah Snake Grass

Evaluation on the effects of P. ostreatus spent mushroom compost and BAP hormone towards C. nutans in vitro culture

In vitro Shoot Multiplication from Nodal Explants of Coccinia grandis (L.) Voigt. and It’s Antidiabetic and Antioxidant Activity

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