Factors affecting in vitro plant regeneration of the critically endangered Mediterranean knapweed (Centaurea tchihatcheffii Fisch et. Mey)

Abstract

Habitat destruction has resulted in the extinction of many plant species from the earth, and many more face extinction. Likely, the annual endemic Mediterranean knapweed (Centaurea tchihatcheffii) growing in the Golbasi district of Ankara, Turkey is facing extinction and needs urgent conservation. Plant tissue culture, a potentially useful technique for ex situ multiplication, was used for the restoration of this ill-fated plant through seed germination, micropropagation from stem nodes, and adventitious shoot regeneration from immature zygotic embryos. The seeds were highly dormant and very difficult to germinate. No results were obtained from the micropropagation of stem nodes. However, immature zygotic embryos showed the highest adventitious shoot regeneration on Murashige and Skoog (MS) medium, containing 1 mg l(-1) kinetin and 0.25 mg l(-1) NAA. Regenerated shoots were best rooted on MS medium containing 1 mg l(-1) IBA and transferred to the greenhouse for flowering and seed set. As such, the present work is the first record of in vitro propagation of critically endangered C. tchihatcheffii, using immature zygotic embryos, and is a step forward towards conservation of this indigenous species.

In vitro propagation of the endangered plant Centaurea ultreiae: assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis

“…For the latter species, the best results were obtained with 2.2 lM BA, compared with the 4.44 lM BA used with C. ultreiae. The lowest response was obtained with shoots cultured on 2-iP, and neither zeatin nor kinetin were effective for micropropagation of C. ultreiae, in contrast with the results obtained with C. paui (Cuenca et al 1999), C. tchihatcheffii (Ozel et al 2006) and Eclipta alba (Borthakur et al 2000) in which kinetin was an efficient PGR, or those obtained with Artemisia vulgaris, i.e., substantial formation of shoots on 2-iP (Sujatha and Ranjitha Kumari 2007). In all treatments with cytokinins some shoots were considered unviable either because they were hyperhydric, or because of scarce development.…”

Growth Regulators Effects onIn VitroShoot Regeneration of Sainfoin (Onobrychis SativaLam.)

“…The results of this study clearly reveal the importance of cotyledon node in micropropagation of sainfoin. the results show that optimal combinations of BAPand nAA in the MS medium could play effective role in enhancing shoot (9), cotyledon node in cowpea (1), thymbra spicata (4), Hungaria vetch (13), Narbon vetch (6), wild lentil (15) and lentil (7) and using different cytokinin and auxins in the MS regeneration medium. The rooting of the plants was not difficult; however, the plantlets showed variations as per pre-treatment during acclimatisation procedure.…”

Tehlike Altındaki Endemik Bitki Centaurea zeybekii Wagenitz ‘nin Kallus İndüksiyonu

“…The low percentage of shoot rooting was also recorded in other reports on Centaurea species [6,7,24].…”