Factors Affecting Haploid Plant Regeneration from Maize Anther Culture

Tsay, Hsin‐Sheng; Miao, S. H.; Widholm, Jack M.

doi:10.1016/s0176-1617(86)80213-9

Cited by 35 publications

(8 citation statements)

References 10 publications

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“…As an example, we found that Tsay et al (1986) kept productive calluses of Zea mays for one year. Few authors mention time spans for embryogenic and/or organogenic capacity of calluses.…”

Section: Discussionmentioning

confidence: 84%

Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries

Martı́nez

Halac

1995

Plant Cell Tiss Organ Cult

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Anthers of O. hookeri containing uninucleate microspores were cultured, in vitro, at 25 ° C ( 16 hours photoperiod) on solid MS medium. After 10-15 days, on media with 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid and 6-benzylaminopurine, anthers developed friable calluses. After unsuccessful treatments on embryogenic -and/or organogenic -induction media, calluses were placed on a hormone-free MS medium for 24 months with routine transfers every 3 weeks. After this period, the calluses developed buds and subsequently plants. Ro generation plants, were morphologically distinct.

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“…As an example, we found that Tsay et al (1986) kept productive calluses of Zea mays for one year. Few authors mention time spans for embryogenic and/or organogenic capacity of calluses.…”

Section: Discussionmentioning

confidence: 84%

Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries

Martı́nez

Halac

1995

Plant Cell Tiss Organ Cult

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“…We compared two cultivation methods using two culture media N6 (Chu 1981) and YP in our experiments. N6 and YP media are the most often used media for microspore initiation in maize anther culture (Ting et al 1981;Genovesi and Collins 1982;Petolino and Jones 1986;Tsay et al 1986;Petolino et al 1988;Barloy et al 1989;Büter et al 1993;Barnabás et al 1999;Nageli et al 1999;Aulinger et al 2003;Jäger et al 2005;Ambrus et al 2006;Pret'ová et al 2006). A direct comparison of both media has not been performed yet.…”

Section: Discussionmentioning

confidence: 99%

Enzyme fingerprint analyses in tissue regenerated from anther culture of maize

et al. 2008

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The objectives of our studies were to investigate the effect of cold pre-treatment duration and the effect of two different culture media (YP and N6) on maize anther culture response in two maize genotypes (A 18 and A 19) and to identify the gametic origin of the maize regenerants. Androgenic induction and callus formation was compared in anther cultures following pre-treatment applied to both media tested and with both maize genotypes. Higher plant regeneration was observed in case of YP media independently of the genotype used. The best results were achieved when 12 days (genotype A 18) or 14 days (in case of genotype A 19) cold pre-treatment at 10°C was applied. We have tested the possibility of using enzyme isoform analyses to identify the microspore origin of calli and plants derived from anther cultures. The 11 enzymes tested in our experiments were acid phosphatase, alcohol dehydrogenase, catalase, diaphorase, b-glucosidase, glutamate oxaloacetate transaminase, isocitrate dehydrogenase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase and phosphoglucoisomerase. Analysis of malate dehydrogenase proved the gametic origin of the calli initiated and of the DH plants regenerated from anther culture, when the coleoptile of the donor plant material showed two forms of enzyme 3/6 and the analysed calli showed only one of the two forms (3 or 6).

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“…(1989) for a highly androgenic genotype of maize (139/39-02) . The combination of 2,4 D and Dicamba, however, also reported to be effective in androgenesis for several maize genotypes (Pescitelli et al ., 1989) or the application of 2,4 D only, as used by Tsay et al . (1986) were ineffective .…”

Section: Discussionmentioning

confidence: 99%

“…Ku et al ., 1978) . Since then, notable progress has been made in the area of anther culture of maize, especially by the new knowledge of nutrient requirements (Ku et al ., 1978), pretreatments (Genovesi & Collins, 1982) and the selection of responsive genotypes (Brettell et al ., 1981 ;Dieu & Beckert, 1986 ;Petolino & Jones, 1986;Petolino & Johnson, 1987 ;Tsay et al ., 1986 ;Vergne et al ., 1990 ;MacDonald, 1992 ; for a review see Genovesi, 1990) .…”

Section: Introductionmentioning

confidence: 99%