Factors Affecting Determination of Protein-Bound Iodine in Serum

Abstract

In order to ensure success with Chaney's method for the determination of protein-bound iodine, a study was made of the factors involved. The chemistry of the reactions was studied, and the effect of variations in method and the kinetics of the ceric sulfate-arsenious acid reaction was determined. The resulting information makes possible successful use of the method, even by technicians with limited training in chemistry.

Quantitative semiautomated colorimetric determination of thyroid (iodine) in thyroid tablets

“…This effect also was observed and demonstrated by Moran (8). One is the increase in absorbance as the temperature is increased.…”

Analytical application of square wave voltammetry

“…The resultant technique of oscilloscopic square wave polarography (3, 4) has achieved only moderate success, and the detection limits obtained were only about those of dc polarography, because of interference from the double layer current resulting from the rapid dc ramp. Mann (5,6) has demonstrated the utility of a staircase potential sweep function in the reduction of double layer current in linear sweep voltammetry. This discontinuous sweep function allowed rapid sweep rates while maintaining the current waveforms of linear sweep voltammetry.…”

Low Dietary Iodine Intake and the Functional Activity of the Rat Thyroid

“…' PBI: At the end of the three month experimental period, blood samples were collected by heart puncture and serum obtained for PBI determinations by the method of Moran (1952). This was based on dry ashing and consisted of use of anionic exchange resins and…”