Translocation of radioactive carbon from the fed part of a blade to the rest of the plant was impaired by a deficiency in phosphorus only when the level of phosphorus was low enough to decrease growth. 50-7209 (17). Cuttings of all varieties were started outdoors in volcanic black sand. Shoots were transplanted at about 2 months of age to tap water culture. The roots were aerated continuously. The plants were grown singly in 8-liter pots. Nutrient solutions prepared with distilled or deionized water were started after about 1 month and renewed at intervals. The nutrient solutions for most of the experiments contained 32, 2, or 0 mg/liter P. The control plants (32 mg/liter P) received a complete solution with the following composition in millimoles per liter: KH2PO4, 1; KNO3, 3; K2SO4, 2; MgSO4-7H20, 1; CaCl2-2H20, 1; and urea, 3.5; as well as ferrous sulfate and other trace elements. In the low P (2 mg/liter P) and -P series (0 mg/liter P) the reduction in KH2PO4 was not compensated for by any other salt, since abundant K was provided by the other salts. The nutrient solutions for the second experiment reported in Table IV contained 2 mmoles of KH2PO4 per liter (64 mg/liter P) and 0.5 mmoles (16 mg/ liter P).Equal doses of '4C02 were supplied to equal lengths of blades of intact plants where grown in sunlight or to portions of detached blades in the constant temperature room at 2000 ft-c. Translocation was measured for periods ranging from 10 min to 6 hr and was terminated by cutting the plant into the required parts. The parts were weighed and sampled. Dried, milled samples were counted at infinite thickness, and the results are expressed as relative specific radioactivity (the net counts per min at infinite thickness), relative total counts (the relative specific radioactivity times the total dry weight in milligrams), and the percentage of relative total counts in the plant (obtained by adding the relative total counts of each part).Determinations of P were performed with the Klett-Summerson colorimeter with the use of the Fiske reagent by methods detailed elsewhere (13). Total P was hydrolyzed for 1 hr with 10 N H2SO at 150 C. Inorganic P was determined on acid extracts.The experiments with detached blades were conducted in duplicate. The other experiments were not replicated.RESULTS AND DISCUSSION P Supply and Translocation of "4C in Entire Plants. Plants of the variety H 37-1933, grown without P, translocated lower percentages of "4C from the fed part than the controls (Table I). The difference was small in young plants but became greater with age. The velocity of translocation was also less in the -P than in the controls. Furthermore, the velocity increased with age in the controls, but decreased with age in the -P. These 569 www.plantphysiol.org on May 11, 2018 -Published by Downloaded from