Facilitating the indirect detection of genomic DNA in an electrochemical DNA biosensor using magnetic nanoparticles and DNA ligase

Hushiarian, Roozbeh; Yusof, Nor Azah; Abdullah, Abdul Halim; Ahmad, Shahrul Ainliah Alang; Dutse, Sabo Wada

doi:10.1016/j.ancr.2015.10.004

Cited by 20 publications

(11 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other work had also been conducted by extracting the DNA of the infected seedlings. Hushiarian et al reported that the detection could be performed using magnetic nanoparticles as immobilized DNA via amide linkages [138,139]. Meanwhile, Dutse et al worked on ruthenium complexes as redox indicators and helped in the intercalation of DNA [140,141].…”

Section: Nanosensor and Detection In Oil Palm Plantationmentioning

confidence: 99%

An Overview of the Oil Palm Industry: Challenges and Some Emerging Opportunities for Nanotechnology Development

2020

View full text Add to dashboard Cite

The increase in the world’s oil demand due to the rise of the global population urges more research into the production of sustainable vegetable oilseeds, among which palm oil is the most suitable candidate as it is the most efficient oilseed crop in the world. In an effort to drive the oil palm industry in the areas of food safety and security nanotechnology could offer a sustainable alternative. However, the utilization of nanotechnology in the oil palm industry is still limited. In this review, we aim to encourage the researchers to fully utilize nanotechnology as an alternative solution to tackle the challenges faced by the oil palm industry. Moreover, we also aim to highlight the opportunities for nanotechnology development in oil palm-based related research. The major points are as follows: (1) Nanosensing enables real-time monitoring of plantation status and crop progression, including soil, water and nutrient management, early pest/disease detection, and the spreading of pests/diseases. The use of nanosensing conveniently extends into advanced breeding topics, such as the development of disease-tolerant plants; (2) Nanotechnology could be the answer for the development of integrated management of pest and disease. Active agricultural ingredients can be entrapped or encapsulated into nanocarrier systems to improve their solubility, stability, enhance their efficient delivery to site-specific targets, with longer shelf life, and consequently improved efficacy; (3) Valuable nanomaterials can be isolated and generated from oil palm biomass waste. The utilization of oil palm biomass waste could overcome the issue of the massive production of waste in the oil palm industry and palm oil mills, where oil only accounts for 10% of the biomass, while 90% is comprised of the generated biowastes. (4) Palm oil can be utilized as a green alternative as a capping and stabilizing agent in the biosynthesis of metallic and non-metallic nanoparticles. In addition, nanoemulsion formulations using palm oil in drug delivery systems offer advantages such as low toxicity, enhance bioavailability and solubility of the drugs, apart from being inexpensive and environmentally friendly.

show abstract

Section: Nanosensor and Detection In Oil Palm Plantationmentioning

confidence: 99%

An Overview of the Oil Palm Industry: Challenges and Some Emerging Opportunities for Nanotechnology Development

2020

View full text Add to dashboard Cite

show abstract

“…Later on, an ultrasensitive electrochemical DNA biosensor based on in situ labeling Au nanoparticles on the free terminal of hairpin DNA was developed, with good regeneration, stability, and hybridization selectivity [123]. Another paper describes Au nanoparticles being used to enhance electrochemical DNA biosensor, where the goal was to improve the sensitivity of biosensor by detecting small sequences in large amounts of double-stranded DNA [124]. Magnetic fluorescent core-shell nanoparticles immobilized with COX were prepared to be used as a sensing material with application in multiparameter fiberoptic biosensor based on enzyme catalysis and oxygen consumption [125].…”

Section: Magnetic Nanoparticles In Biosensorsmentioning

confidence: 99%

Micro‐ and Nanocarriers for Immobilization of Enzymes

Leitgeb¹,

Knez²,

Vasić³

2016

Micro and Nanotechnologies for Biotechnology

View full text Add to dashboard Cite

Two types of micro-and nanocarriers for immobilization of enzymes for biotechnological and biomedical applications are described: magnetic nanoparticles and crosslinked enzyme aggregates (CLEAs). Nanosized structures with their large surface and smaller size volume ratio, which is dependent on their strong magnetic dipole, give key features that make magnetic nanoparticles useful in many biotechnological and biomedical applications. They are therefore used as carriers to which different active substances can bind. The preparation of the magnetic nanoparticles, possible surface coating methods, and functionalization with different materials are described. Enzyme immobilization methods, such as adsorption, affinity binding, chelation, or metal binding or covalent binding, enable the preparation of efficient and stable enzyme bound to magnetic nanoparticles. Such a product may be used among bioreactor applications for targeted drug delivery in biosensors or bioimaging and magnetic resonance imaging. Preparation of CLEAs, the microsized enzyme structures without a carrier, is described as well. Their main advantage is very simple preparation, where two steps, precipitation of the enzyme and cross-linking, are joined. A broad spectrum of enzymes for CLEA preparation has been used and many biotechnological reactions are catalyzed. The improvement in CLEA preparation to enhance their stability and operability is also shown.

show abstract

“…Biosensing strategies are showing great promise with such features as being time-saving, cost-effective, practical, and able to perform real-time analysis (Fernandes et al 2015 ; Hushiarian et al 2015b ; Lu et al 2013 ; Tian et al 2016 ; Zhang et al 2013 ). In the last decade, electrochemical DNA biosensors have revolutionized modern analysis for detecting contaminants in a range of foods and environments (Celik et al 2013 ; Dong et al 2012 ; Dutse et al 2013 ; Hushiarian et al 2015a ; Singh et al 2013 ; Yin et al 2013 ). Numerous electrochemical biosensors, based on screen-printing and with DNA immobilized on their surfaces, have been reported in the scientific literature (Alocilja et al 2013 ; Das et al 2014 ; Ding et al 2012 ; Pal and Alocilja 2010 ; Paniel and Baudart 2013 ) and have been successfully employed for the fabrication of electrodes for mass production of disposable, low-cost devices (Caramit et al 2015 ; Monteiro et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus

et al. 2017

Self Cite

View full text Add to dashboard Cite

An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10−8–2.0 × 10−13 M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool.

show abstract

Facilitating the indirect detection of genomic DNA in an electrochemical DNA biosensor using magnetic nanoparticles and DNA ligase

Cited by 20 publications

References 28 publications

An Overview of the Oil Palm Industry: Challenges and Some Emerging Opportunities for Nanotechnology Development

An Overview of the Oil Palm Industry: Challenges and Some Emerging Opportunities for Nanotechnology Development

Micro‐ and Nanocarriers for Immobilization of Enzymes

A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus

Contact Info

Product

Resources

About