Facilitated permeation of methyl viologen into chloroplasts in situ during electric pulse generation in excitable plant cell membranes

Булычев, А. А.; Krupenina, Natalia A.

doi:10.1134/s1990747808040132

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…This is in line with previous reports that MV does not permeate through the plasma membrane of Chara and remains inaccessible for chloroplasts, at least in the areas with slightly acid external pH [41]. However, MV was shown to permeate immediately into chloroplasts and switch over the electron transport routes after a single elicitation of the action potential [41,42]. Our present experiments confirm that MV becomes immediately accessible to chloroplasts after a single AP generation.…”

Section: Role Of Natural Electron Carriers On the Acceptor Side Of Psisupporting

confidence: 94%

“…8B). Strong light-induced quenching of F m ′ under low-irradiance illumination is typical of MV-treated cells [41,42]. In the presence of MV the rapid drops in F′ and F m ′ were coincident, indicating that the drop in F′ was largely determined by non-photochemical quenching.…”

Section: Role Of Natural Electron Carriers On the Acceptor Side Of Psimentioning

confidence: 92%

“…The CO 2 -dependent electron flow is mediated by FNR whose photoactivation is manifested in transient changes of chlorophyll P700 redox state [33]. Methyl viologen was found to eliminate characteristic FNR-mediated absorbance changes of P700 + at 810 nm in Chara cells [42]. Judging from the absorbance changes ΔA 810 , FNR is activated in C. corallina within few seconds of illumination, rather than after illumination periods~60 s required for the photoinduction of cyclosis-mediated interactions.…”

Section: Role Of Natural Electron Carriers On the Acceptor Side Of Psimentioning

confidence: 97%

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Photoinduction of cyclosis-mediated interactions between distant chloroplasts

Булычев

Комарова

2015

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Communications between chloroplasts and other organelles based on the exchange of metabolites, including redox active substances, are recognized as a part of intracellular regulation, chlororespiration, and defense against oxidative stress. Similar communications may operate between spatially distant chloroplasts in large cells where photosynthetic and respiratory activities are distributed unevenly under fluctuating patterned illumination. Microfluorometry of chlorophyll fluorescence in vivo in internodal cells of the alga Chara corallina revealed that a 30-s pulse of localized light induces a transient increase (~25%) in F' fluorescence of remote cell parts exposed to dim background light at a 1.5-mm distance on the downstream side from the illuminated spot in the plane of unilateral cytoplasmic streaming but has no effect on F' at equal distance on the upstream side. An abrupt arrest of cytoplasmic streaming for about 30s by triggering the action potential extended either the ascending or descending fronts of the F' fluorescence response, depending on the exact moment of streaming cessation. The response of F' fluorescence to localized illumination of a distant cell region was absent in dark-adapted internodes, when the localized light was applied within the first minute after switching on continuous background illumination of the whole cell, but it appeared in full after longer exposures to continuous background light. These results and the elimination of the F' response by methyl viologen known to redirect electron transport pathways beyond photosystem I indicate the importance of photosynthetic induction and the stromal redox state for long-distance communications of chloroplasts in vivo.

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Section: Role Of Natural Electron Carriers On the Acceptor Side Of Psisupporting

confidence: 94%

Section: Role Of Natural Electron Carriers On the Acceptor Side Of Psimentioning

confidence: 92%

Section: Role Of Natural Electron Carriers On the Acceptor Side Of Psimentioning

confidence: 97%

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Photoinduction of cyclosis-mediated interactions between distant chloroplasts

Булычев

Комарова

2015

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Membrane Excitation and Cytoplasmic Streaming as Modulators of Photosynthesis and Proton Flows in Characean Cells

Булычев

2012

Plant Electrophysiology

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Chlorophyll fluorescence images demonstrate variable pathways in the effects of plasma membrane excitation on electron flow in chloroplasts of Chara cells

2010

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Chlorophyll fluorescence Imaging and Microscopy PAM fluorometry were applied to study spatial dynamics of photosystem II quantum yield (ΔF/F'(m)) and non-photochemical quenching (NPQ) in resting and electrically stimulated Chara corallina cells in the absence and presence of the hydrophilic electron acceptor methyl viologen (MV) in the external medium. Electrical excitation of the plasma membrane temporarily enhanced the heterogeneity of photosynthetic patterns under physiological conditions (in the absence of MV), but irreversibly eliminated these patterns in the presence of MV. These findings suggest that the action potential (AP) of the excitable plant cell affects the spatial patterns of photosynthesis and chlorophyll fluorescence through different pathways operated in the absence and presence of MV. Based on the extent of NPQ as an indicator of MV-dependent electron flow, it is supposed that MV cannot permeate into the chloroplasts of photosynthetically active "acid cell regions" but gains an immediate access to the stroma of these chloroplasts after triggering of an AP. The AP-triggered MV-dependent non-photochemical quenching in the chloroplasts of acidic cell regions was routinely observed at 0.1 mM Ca(2+) in the medium but not at elevated (2 mM) external Ca(2+) concentration. The results are interpreted in terms of competition between two permeant divalent ion species, Ca(2+) and MV(2+), for their passage through the voltage-gated calcium channels of the plasma membrane. It is proposed that the herbicidal activity of MV in characean cells, here serving as model object, can be manipulated by triggering AP and varying Ca(2+) concentration in the environmental medium.

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Facilitated permeation of methyl viologen into chloroplasts in situ during electric pulse generation in excitable plant cell membranes

Cited by 4 publications

References 20 publications

Photoinduction of cyclosis-mediated interactions between distant chloroplasts

Photoinduction of cyclosis-mediated interactions between distant chloroplasts

Membrane Excitation and Cytoplasmic Streaming as Modulators of Photosynthesis and Proton Flows in Characean Cells

Chlorophyll fluorescence images demonstrate variable pathways in the effects of plasma membrane excitation on electron flow in chloroplasts of Chara cells

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