Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells

Abstract

Cancer is one of the most dangerous threats to human health and possibly the utmost task for current medicine. Currently, bio-based synthesis of nanoparticles from plants has gained much interest due to its potential medicinal applications. In the present study, a biological approach was employed for biogenic (green) synthesis of silver nanoparticles (AgNPs) using dried leaf extract of Asian spider flower (Asf). The biogenic synthesis of Asf-AgNPs (Asian spider flower-Silver nanoparticles) was established using ultra violet-visible (UV-vis) spectra which exhibited a wide superficial plasmon resonance of AgNPs at 445 nm. These nanoparticles clearly showed the formation of poly-disperse crystalline solids (spherical shape) with particle size range of <50 nm based on observation under a transmission electron microscope (TEM). Infrared spectroscopy (FTIR) revealed carboxylic acids (C = O stretch) known to act as a capping agent and a reductant in plant extracts. Elemental silver signal peak was observed in the graph obtained from energy-dispersive X-ray (EDX) analysis. Biocompatibility tests for Asf-AgNPs at different doses were evaluated against human breast cancer cells (MCF7) for cell viability and apoptotic analysis. According to the evaluation, biosynthesized Asf-AgNPs could prevent the explosion of human breast tumor cells (MCF7) in IC50 at a dose of 40 μg/mL after 48 h of treatment. The results obtained in the IC50 dosage treatments were statistically significant (p < 0.05) when compared with control. Nuclear damage of cells was further investigated using annexin V-FITC/PI dual staining and DAPI (4′,6-diamidino-2-phenylindole) staining method. Bright blue fluorescence with condensed and fragmented chromatin was observed. Western blot analysis showed increased expression levels of caspases-3 and 9 (apoptotic proteins). These results indicate that bio-approached AgNPs synthesized through Asf plant extract could be used as potential therapeutic medications for human cancer cells.

Review on Silver Nanoparticle Synthesis Method, Antibacterial Activity, Drug Delivery Vehicles, and Toxicity Pathways: Recent Advances and Future Aspects

“…The adsorbed surfactant molecules from the solution prevent the coalescence of particles and control the rate of particle exaggeration. By choosing the proper kind or concentration of the surface active agent, the size of particles composed in the solution can be contained on a nanometer scale [111][112][113][114][115][116][117]. A process patent has been granted to Li and Ong [118] for the preparation of silver nanoparticles and the formation of molecules of an initial stabilizer on the surface of the nanoparticles, as well as the mixing of a replacement stabilizer composed mainly of a carboxylic acid also with composition to substitute at least a portion of the primary stabilizer with the replacement stabilizer, resulting in molecules of the initial stabilizer on the surface of the nanoparticles [119][120][121][122][123][124][125][126][127][128][129][130].…”

Green Synthesis of a Novel Silver Nanoparticle Conjugated with Thelypteris glandulosolanosa (Raqui-Raqui): Preliminary Characterization and Anticancer Activity

“…The potential of plant-based nanoparticles to treat different cancers is being extensively studied and well reviewed [76] and the application of silver and gold nanoparticles to treat cancers is a very active area of research investigations [76][77][78][79][80]. Recent studies revealed that the use of the various metallic nanoparticles in medical sciences has various applications, such as bio-imaging [81][82][83][84], bio-sensing [85][86][87], and drug delivery [88][89][90] of nutraceuticals [23,26,[91][92][93][94][95][96][97][98][99], and pharmaceuticals [17,20,31,100], which could affect disposition [13,24,25,[27][28][29][30]32,33,[101][102][103][104][105][106][107]…”

Electrochemical properties of biogenic silver nanoparticles synthesized using Hagenia abyssinica (Brace) JF. Gmel. medicinal plant leaf extract

“…In recent years, Ag nanoparticles (Ag NPs) have attracted much attention of researchers due to their wide applications in biotechnology, biomedicine and other industries. Because of chemical stability, good conductivity, catalytic nature, nano silver finds applications as an antimicrobial agent, in textiles, home water purification systems, medical devices, cosmetics, electronics, and household appliances [1]. In addition, due to its conducting nature, its electrochemical properties are worthy to investigate.…”