Facile synthesis of multiple enzyme-containing metal–organic frameworks in a biomolecule-friendly environment

Wu, Xiaoling; Ge, Jun; Yang, Cheng; Hou, Meng; Liu, Zheng

doi:10.1039/c5cc05136c

Cited by 488 publications

(367 citation statements)

References 47 publications

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“…Examples are the preparations of hydrogels, 15,22 hourglass shaped nanochannel reactor, 23 mesoporous silica nanoparticles, 18,24 formation of inorganic nanocrystal-protein complexes 25 and self-assembled crystals, 26 microbeads, 27,28 DNA nanostructures, 4,5,11,[29][30][31] polymersomes, 14,32 nanoparticles, 33 nanobers, 13,16,34 graphene, 9,35 and metal-organic frameworks (MOFs). 17,36,37 In contrast to other scaffolds, MOFs, which are formed by the self-assembly of metal ions and organic linkers, feature ultrahigh surface area and porosity, uniform pores with tunable sizes, surfaces with variable chemistries, and structural diversity. 38 The large surface area and uniform pore sizes of MOFs enhance capacity for enzyme immobilization and facilitate mass transport.…”

Section: Introductionmentioning

confidence: 99%

Magnetic metal–organic frameworks as scaffolds for spatial co-location and positional assembly of multi-enzyme systems enabling enhanced cascade biocatalysis

et al. 2017

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Magnetic multi-enzyme nanosystems have been prepared via co-precipitation of enzymes and metalorganic framework HKUST-1 precursors in the presence of magnetic Fe 3 O 4 nanoparticles. The spatial co-localization of two enzymes was achieved using a layer-by-layer positional assembly strategy.Glucose oxidase (GOx) and horseradish peroxidase (HRP) were used as the model enzymes for cascade biocatalysis. By controlling the spatial positions of enzymes, three bienzyme nanosystems GOx@HRP@HKUST-1@Fe 3 O 4 , GOx-HRP@HKUST-1@Fe 3 O 4 and HRP@GOx@HKUST-1@Fe 3 O 4 were prepared in which GOx and HRP containing layers were in close proximity, either encapsulated in the HKUST-1 inner layer, or immobilized on the HKUST-1 outer shell, or randomly distributed in the two MOF layers. Their properties were characterized by transmission electron microscopy, energydispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermal gravimetric analysis, and zeta potential measurements. The highest activity was observed at pH ¼ 6 and a temperature of 20 C. Thanks to the favorable positioning of enzymes, the GOx@HRP@HKUST-

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Section: Introductionmentioning

confidence: 99%

Magnetic metal–organic frameworks as scaffolds for spatial co-location and positional assembly of multi-enzyme systems enabling enhanced cascade biocatalysis

et al. 2017

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“…It is widely used in forage, medicine, and other fields (Wong et al 2008). In recent years, in order to overcome the disadvantages of the free GOx such as poor mechanical stability, difficult separation, and non-recyclability (Cao et al 2016), several nanoparticles, such as titanium dioxide nanotubes (Ravariu et al 2011), Fe 3 O 4 /APTES (França 2014), Ag@Zn-TSA (Dong et al 2016), and ZIF-8 (Wu et al 2015) were attempted to be used as enzyme carriers for the immobilization of GOx. Among these nanoparticles, carriers containing metal-organic frameworks (MOFs) have received more and more concern because of their excellent physical and chemical properties mentioned above.…”

Section: Introductionmentioning

confidence: 99%

Magnetic ZIF-8/cellulose/Fe3O4 nanocomposite: preparation, characterization, and enzyme immobilization

Cao

Lai

et al. 2017

Bioresour. Bioprocess.

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Background:The ZIF-8-coated magnetic regenerated cellulose-coated nanoparticles (ZIF-8@cellu@Fe 3 O 4 ) were successfully prepared and characterized. The result showed that ZIF-8 was successfully composited on to the surface of the cellulose-coated Fe 3 O 4 nanoparticles by co-precipitation method. Moreover, the glucose oxidase (GOx, from Aspergillus niger) was efficiently immobilized by the ZIF-8@Cellu@Fe 3 O 4 nanocarriers with enhanced catalytic activities. The enzyme loading was 94.26 mg/g and the enzyme activity recovery was more than 124.2%. This efficiently immobilized enzyme exhibits promising applications in biotechnology, diagnosis, biosensing, and biomedical devices. Conclusions:A new core-shell magnetic ZIF-8/cellulose nanocomposite (ZIF-8@Cellu@Fe 3 O 4 ) was fabricated and structurally characterized. Glucose oxidase (GOx) was successfully immobilized by the biocompatible ZIF-8@Cellu@

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“…In the co-immobilization system, the reaction intermediates could rapidly reach the next active site, 39,5 so that the overall activity was improved. Other methods, such as dual-functionalized sequential colocalization, 39 and metal-organic framework nanocrystals, 40 were also used to co-localize GOx and HRP, enhancing the overall product conversion by two-fold compared to the controls. At pH 9 and 10, however, we did not observe much enhancement.…”

mentioning

confidence: 99%

Co-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detection

et al. 2016

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Preserving enzyme activity and promoting synergistic activity via co-localization of multiple enzymes are key topics in bionanotechnology, materials science, and analytical chemistry.This study reports a facile method for co-immobilizing multiple enzymes in metal coordinated hydrogel nanofibers. Specifically, four types of protein enzymes, including glucose oxidase, Candida rugosa lipase, α-amylase, and horseradish peroxidase were respectively encapsulated in a gel nanofiber made of Zn 2+ and adenosine monophosphate (AMP) with a simple mixing step. Most enzymes achieved quantitatively loading and retained full activity. At the same time, the entrapped enzymes were more stable against temperature variation (by 7.5 C), protease attack, extreme pH (by 2-fold), and organic solvents. After storage for 15 days, the entrapped enzyme still retained 70% activity while the free enzyme nearly completely lost activity. Compared to nanoparticles formed with AMP and lanthanide ions, the nanofiber gels allowed much higher enzyme activity. Finally, a highly sensitive and selective biosensor for glucose was prepared using the gel nanofiber to co-immobilize glucose oxidase and horseradish peroxidase for an enzyme cascade system. A detection limit of 0.3 µM glucose with excellent selectivity was achieved. This work indicates that metal coordinated materials using nucleotides are highly useful for interfacing with biomolecules.3

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Facile synthesis of multiple enzyme-containing metal–organic frameworks in a biomolecule-friendly environment

Cited by 488 publications

References 47 publications

Magnetic metal–organic frameworks as scaffolds for spatial co-location and positional assembly of multi-enzyme systems enabling enhanced cascade biocatalysis

Magnetic metal–organic frameworks as scaffolds for spatial co-location and positional assembly of multi-enzyme systems enabling enhanced cascade biocatalysis

Magnetic ZIF-8/cellulose/Fe3O4 nanocomposite: preparation, characterization, and enzyme immobilization

Co-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detection

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