Facile synthesis of multi‐walled carbon nanotube via folic acid grafted nanoparticle for precise delivery of doxorubicin

Abstract

The motive of work was to develop a multi-walled carbon nanoplatform through facile method for transportation of potential anticancer drug doxorubicin (DOX). Folic acid (FA)-ethylene diamine (EDA) anchored and acid functionalised MWCNTs were covalently grafted with DOX via π-π stacking interaction. The resultant composite was corroborated by 1 H NMR, FTIR, XRD, EDX, SEM, and DSC study. The drug entrapment efficiency of FA-conjugated MWCNT was found high and stability study revealed its suitability in biological system. FA-EDA-MWCNTs-DOX conjugate demonstrated a significant in vitro anticancer activity on human breast cancer MCF-7 cells. MTT study revealed the lesser cytotoxicity of folate-conjugated MWCNTs. The obtained results demonstrated the targeting specificity of FA-conjugate via overexpressed folate receptor deemed greater scientific value to overcome multidrug protection during cancer therapy. The proposed strategy is a gentle contribution towards development of biocompatible targeted drug delivery and offers potential to address the current challenges in cancer therapy.

Lysinated Multiwalled Carbon Nanotubes with Carbohydrate Ligands as an Effective Nanocarrier for Targeted Doxorubicin Delivery to Breast Cancer Cells

“…This was due to a chloride substitution in the carbonyl group, which has a lone pair of electrons, resulting in a π-π* transition and consequently, the peak was shifted to a lower wavelength by the inductive effect. Similarly, UV absorbance data for the conjugates have been reported in previously published studies [ 11 , 21 , 34 ]. COClMWCNTs had an absorption peak at 235 nm.…”

Carbon Nanotubes: Smart Drug/Gene Delivery Carriers

“…Additionally, the FA-EDA-MWCNTs-DOX compound demonstrated 7.58% cytotoxicity for a 1 μg/mL concentration and 30.31% cytotoxicity at 100 μg/mL. 100 PTX is considered an effective anti-cancer drug, but it is insoluble and has a tendency to accumulate. The combination of this drug by covalent and non-covalent bonds with CNTs provides a new platform for cancer therapy.…”

Advances in Drug Delivery Nanosystems Using Graphene-Based Materials and Carbon Nanotubes

“…In cells exposed to MWCNT-COOH-CDDP, considerably reduced expression of caspase-3 and p53 and downregulation of NF-κB was observed after 48 h; this system stimulated apoptosis escape and was not capable of overcoming the triple negative breast cancer cell resistance [292]. FA-ethylene diamine (ED)-anchored MWCNT-COOH covalently grafted with DOX via π-π stacking interaction showed strong in vitro anticancer activity on MCF-7 cells and excellent targeting specificity via overexpressed folate receptor but lower cytotoxicty [293]. pH-Responsive release at low pH values, targeting and causing strong growth inhibition of tumor cells overexpressing FA receptors, exhibited also nanocomplexes of DOX and multifunctional FA-bound MWCNTs fabricated using MWCNT-COOH covalently conjugated with PEI and subsequently stepwise modified with FA, fluorescein isothiocyanate and acetic anhydride/triethylamine.…”