Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity

The present study deals with the ecofriendly one-pot synthesis and stabilization of silver nanoparticles (AgNPs) by using aqueous extract of Mentha longifolia branches. Spectrophotometric analysis of different ratios of reactants revealed that a 1 to 9 ratio of plant extract and silver salt solution respectively is the most suitable proportion for synthesis. Synthesis of AgNPs was confirmed initially by the observation of change in the color of the reaction mixture which was carried out at 60°C by using 3 mM of silver salt and the pH of the reaction medium was maintained at 5.22. A characteristic surface plasmon resonance (SPR) band was observed at 495 nm of light wavelength. SEM images revealed that the nanoparticles are in ∼20-80 nm and are anisotropic and nearly spherical while EDX analysis showed the presence of elemental Ag with ∼90% signal intensity. Size distribution analysis of AgNPs was performed by dynamic light scattering technique and AgNPs were found in the range of ∼8-30 nm. ROS quantification revealed that the AgNPs have a quantum yield of 0.09 Φ which provides them the ability to proteolytically treat cancer and other microbial pathogenic cells. AgNPs did not report any photothermal activity to be used as photodynamic agents. These findings explain the redox potential of M. longifolia to bio-fabricate AgNPs and their abilities to generate ROS may help to curb dreading diseases.

“…A narrow and symmetrical peak showed that the nanoparticles are monodisperse and spherical [13]. Our results are in favor of some previously published reports [7,27,28].…”

Section: Phytosynthesis Of Agnps (Spectrophotometric Studies)supporting

confidence: 53%

One-pot phytosynthesis of nano-silver from Mentha longifolia L.: their characterization and evaluation of photodynamic potential

Javed

Nadhman

Razzaq

et al. 2020

“…Biosynthesis of NPs is preferred over other methods owing to its biocompatibility, low cost, nontoxicity and mainly due to environmentally friendly properties [46][47][48]. Plants leaves and fruits have anciently been used as medicine because they are rich in antibiotics, flavonoids, amino acids, terpenoids, steroids, alkaloids, and polysaccharides that are used for the production of useful herbal medicines for various diseases including cancer [49,50]. Strong antimicrobial property of the herbal medicines is always required because of resistance of microbes to the antibiotic which is still a major issue in medical science.…”

Section: Discussionmentioning

confidence: 99%

Flacourtia indica based biogenic nanoparticles: development, characterization, and bioactivity against wound associated pathogens

Ahmad

Taj

Ramzan

et al. 2020

Surface engineered nanoparticles (NPs) are of great attention due to their targeted medical applications. The nature of the functionalized surface plays a vital role in achieving the required functionalities of engineered NPs. Owing to the biofilm formation capabilities of wound associated pathogens, impaired wound healing is a major complication in the medical field. In this context, herein, we report the biogenic synthesis of Flacourtia indica (FI) based NPs, i.e., FI-AgNPs using the aqueous leaf extract of this anti-bacterial herb. The newly developed FI-AgNPs were characterized using various analytical and imaging techniques such as UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), powder X-ray diffraction (PXRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The FI-AgNPs showed potent anti-microbial activity and anti-biofilm potential which were examined through a test tube adherence method and congo red agar method. It has been observed that synthesized FI-AgNPs inhibit the formation of a biofilm of observed bacteria, even at a minimum concentration of 80 μgml −1 . These findings suggest that synthesized FI-AgNPs could be used against wound associated microbes, especially bacterial coating on medical devices, to prevent antibiotic-resistant biofilm infections. Further development and research are obligatory to decode this skill into preventive and therapeutic strategies.

“…The use of extracts of leaves, flowers and essential oils can act as chemical agents of metallic salts and stabilizers of the system due to its components as amino acids, flavonoids, phenolic compounds, terpenoids, among others. Related to the production of nanoparticles using plant phytochemicals, the plant extract mixed up with a solution of metal salt and the reaction carry out completely in a few minutes [12]. However, the contact time, concentration of metal salt, pH, and temperature can effect on the properties of nanoparticles, and these parameters in the syntheses of silver nanoparticles have not been completed investigated.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity, morphology, and physicochemical stability of biosynthesized silver nanoparticles using thyme (Thymus vulgaris) essential oil

Melo

Maciel

Almeida

et al. 2020

Considering the importance of biosynthesized silver nanoparticles (AgNPs) using plant phytochemicals, the aim of this work was to evaluate the effect of pH in the formulation of AgNPs, bioreduced with thyme essential oil. Biosynthesized AgNPs were prepared under different pH (7, 8, 9 and 10), and the physicochemical stability was evaluated during 90 days at 6°C and 25°C. Using GC/MS technique, 17 different compounds were observed in the thyme essential oil; however, borneol and αterpineol were the majoritarian compounds, probably responsible to the formation of AgNPs. UV-vis spectroscopy with absorbance between 415 and 440 nm and Transmission Electron Microscopy (TEM) confirmed the synthesis of nanoparticles, with diameters of 40 nm and spherical shapes to pH 7, 8 and 9. Through DLS approach, was observed that the average particle diameter was around 90 nm for all pH tested. AgNPs presented homogeneous size distribution, and zeta potential values very close to the theoretical stability range. Moreover, biosynthesized AgNPs presented high antibacterial activity against Escherichia coli and Staphylococcus aureus. Through x-ray Photoelectron Spectroscopic (XPS) analysis was possible confirm the presence of crystalline silver nanoparticles. Thus, silver nanoparticles eco-friendly prepared using thyme essential oil can be considered as an alternative way to produce this nanomaterial with great stability and high antibacterial activity.