Facile control of RGD-alginate/hyaluronate hydrogel formation for cartilage regeneration

Park, Honghyun; Lee, Kuen Yong

doi:10.1016/j.carbpol.2011.05.032

Cited by 36 publications

(14 citation statements)

References 37 publications

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“…In a previous study, we demonstrated that RGD‐modified AL/HA formed a cross‐linked structure in the presence of primary chondrocytes and that these hydrogels promoted cartilage tissue development in vivo . The addition of HA enhanced specific interactions with primary chondrocytes due to CD44‐HA interactions, thereby improving cartilage formation compared to when only RGD‐modified AL was used.…”

Section: Introductionmentioning

confidence: 95%

Cartilage regeneration using biodegradable oxidized alginate/hyaluronate hydrogels

Park

Lee

2014

J. Biomed. Mater. Res.

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Despite the widespread use of alginate (AL) hydrogels in many biomedical applications, including tissue engineering, AL is inherently non-degradable under physiological conditions. We hypothesized that degradable alginate (dAL) would be useful for cartilage regeneration when combined with hyaluronate (HA). We prepared dAL by partial oxidation of AL using sodium periodate, and the degradation rate of AL hydrogel was able to be regulated by control of sodium periodate concentration. The degradable oxidized AL/HA gels were formed in the presence of cells and their characteristics were investigated. dAL/HA gels with primary chondrocytes were injected subcutaneously into mice. Effective cartilage regeneration was observed after 6 weeks of transplantation based on histological analysis. Moreover, substantial secretion of sulfated glycosaminoglycans and expression of chondrogenic marker genes were also observed compared with non-dAL/HA gels. These results indicate that dAL/HA hydrogels may be useful in cartilage regeneration, and in many tissue engineering applications.

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Section: Introductionmentioning

confidence: 95%

Cartilage regeneration using biodegradable oxidized alginate/hyaluronate hydrogels

Park

Lee

2014

J. Biomed. Mater. Res.

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“…Various hydrogel-based biomaterials have been widely investigated in the context of cartilage tissue engineering, due to their ability to be used for 3D cell encapsulation, as well as their ease of modification, high water content, injectability, and biocompatibility. 8 For instance, different types of naturally derived or synthetic-based hydrogels, such as chitosan, 9,10 HA, 11−14 polyethylene glycol (PEG), 15 silk, 16−18 collagen, 19,20 alginate, 21,22 and recombinant elastin-like polypeptides (ELPs), 23 have been used to induce cartilage tissue regeneration and repair. 11,24,25 Among the different biomaterials explored for cartilage repair, HA and ELPs have been shown to possess intrinsic properties that promote the regeneration of cartilage tissue.…”

Section: Introductionmentioning

confidence: 99%

Engineering Adhesive and Antimicrobial Hyaluronic Acid/Elastin-like Polypeptide Hybrid Hydrogels for Tissue Engineering Applications

Sani

Portillo-Lara

Spencer

et al. 2018

ACS Biomater. Sci. Eng.

109

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Hydrogel-based biomaterials have been widely used for tissue engineering applications because of their high water content, swellability, and permeability, which facilitate transport and diffusion of essential nutrients, oxygen, and waste across the scaffold. These characteristics make hydrogels suitable for encapsulating cells and creating a cell supportive environment that promotes tissue regeneration when implanted in vivo. This is particularly important in the context of tissues whose intrinsic regenerative capacity is limited, such as cartilage. However, the clinical translation of hydrogels has been limited by their poor mechanical performance, low adhesive strength, uncontrolled degradation rates, and their susceptibility to bacterial colonization. Here, we introduce an elastic, antimicrobial, and adhesive hydrogel comprised of methacrylated hyaluronic acid (MeHA) and an elastin-like polypeptide (ELP), which can be rapidly photo-cross-linked in situ for the regeneration and repair of different tissues. Hybrid hydrogels with a wide range of physical properties were engineered by varying the concentrations of MeHA and ELP. In addition, standard adhesion tests demonstrated that the MeHA/ELP hydrogels exhibited higher adhesive strength to the tissue than commercially available tissue adhesives. MeHA/ELP hydrogels were then rendered antimicrobial through the incorporation of zinc oxide (ZnO) nanoparticles, and were shown to significantly inhibit the growth of methicillin-resistant Staphylococcus aureus (MRSA), as compared to controls. Furthermore, the composite adhesive hydrogels supported in vitro mammalian cellular growth, spreading, and proliferation. In addition, in vivo subcutaneous implantation demonstrated that MeHA/ELP hydrogels did not elicit any significant inflammatory response, and could be efficiently biodegraded while promoting the integration of new autologous tissue. In summary, we demonstrated for the first time that MeHA/ELP-ZnO hydrogel can be used as an adhesive and antimicrobial biomaterial for tissue engineering applications, because of its highly tunable physical characteristics, as well as remarkable adhesive and antimicrobial properties.

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“…In addition to the functions of heparin in sequestering growth factors, the current research demonstrated that incorporation of gelatin conferred cell adhesion. ECM‐derived molecules, such as collagen or gelatin and peptide sequences including RGD, have been commonly used to introduce cell adhesive properties and specific cell interactions to many natural and synthetic hydrogels, resulting in improved cell viability and functionality . The ECM proteins were usually present as a major constituent of the hydrogel matrix, affecting both functional and physical characteristics whereas in this study, the physical properties and permselectivity of hydrogels were not affected by the small percentage of gelatin incorporated.…”

Section: Discussionmentioning

confidence: 75%

Bioactivity of permselective PVA hydrogels with mixed ECM analogues

Nafea

Poole-Warren

Martens

2015

J Biomedical Materials Res

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The presentation of multiple biological cues, which simulate the natural in vivo cell environment within artificial implants, has recently been identified as crucial for achieving complex cellular functions. The incorporation of two or more biological cues within a largely synthetic network can provide a simplified model of multifunctional ECM presentation to encapsulated cells. Therefore, the aim of this study was to examine the effects of simultaneously and covalently incorporating two dissimilar biological molecules, heparin and gelatin, within a PVA hydrogel. PVA was functionalized with 7 and 20 methacrylate functional groups per chain (FG/c) to tailor the permselectivity of UV photopolymerized hydrogels. Both heparin and gelatin were covalently incorporated into PVA at an equal ratio resulting in a final PVA:heparin:gelatin composition of 19:0.5:0.5. The combination of both heparin and gelatin within a PVA network has proven to be stable over time without compromising the PVA base characteristics including its permselectivity to different proteins. Most importantly, this combination of ECM analogues supplemented PVA with the dual functionalities of promoting cellular adhesion and sequestering growth factors essential for cellular proliferation. Multi-functional PVA hydrogels with synthetically controlled network characteristics and permselectivity show potential in various biomedical applications including artificial cell implants.

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Facile control of RGD-alginate/hyaluronate hydrogel formation for cartilage regeneration

Cited by 36 publications

References 37 publications

Cartilage regeneration using biodegradable oxidized alginate/hyaluronate hydrogels

Cartilage regeneration using biodegradable oxidized alginate/hyaluronate hydrogels

Engineering Adhesive and Antimicrobial Hyaluronic Acid/Elastin-like Polypeptide Hybrid Hydrogels for Tissue Engineering Applications

Bioactivity of permselective PVA hydrogels with mixed ECM analogues

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