Facile and label-free fluorescence sensing of β-galactosidase activity by graphene quantum dots

Xie, Xin; Lian, Yawen; Xiao, Lehui; Wei, Lin

doi:10.1016/j.saa.2020.118594

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…GQDs were fluorescent (λexc=370nm; λem=475nm), but in the presence of β-Gal and p-nitrofenol-β-Dgalacto-pyranoside (24), this emission band was significantly reduced due to the hydrolysis of 24 into pnitrofenol and subsequent inner filter effect (IFE), see Scheme 2b. [40] The absorption spectrum of pnitrophenol overlaps with the excitation spectrum of the GQDs, so that in the presence of the hydrolysate of 24, a competitive absorption reaction occurs that weakens the excitation of the GQDs causing fluorescence quenching. The system was able to detect β-Gal in a range from 20 to 200 U L −1 with a detection limit of 4.4 U L −1 .…”

Section: Coormentioning

confidence: 99%

Chromo-fluorogenic probes for β-galactosidase detection

et al. 2021

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β-Galactosidase (β-Gal) is a widely used enzyme as a reporter gene in the field of molecular biology which hydrolyses the β-galactosides into monosaccharides. β-Gal is an essential enzyme in humans and its deficiency or its overexpression results in several rare diseases. Cellular senescence is probably one of the most relevant physiological disorders that involve β-Gal enzyme. In this review, we assess the progress made to date in the design of molecular-based probes for the detection of β-Gal both in vitro and in vivo. Most of the reported molecular probes for the detection of β-Gal consist of a galactopyranoside residue attached to a signalling unit through glycosidic bonds. The β-Gal-induced hydrolysis of the glycosidic bonds released the signalling unit with remarkable changes in colour and/or emission. Additional examples based on other approaches are also described. The wide applicability of these probes for the rapid and in situ detection of de-regulation β-Gal-related diseases has boosted the research in this fertile field. 33, 34 HBSS buffer 570/680 Turn on C6-lacZ 35 PBS (10 mM, pH 7.4) 405/545 Turn on C6-lacZ 36 HBSS buffer 640/680 Turn on HCT116-lacZ 37-39 HEPES (pH 7.3)-DMSO 99.9:0.01 v/v 555/582 Turn on COS-7-lacZ 40 PBS-EtOH/10:1 375/↓450↑5 40 Ratiometric (Twophoton) HEK293-lacZ 41 PBS 430/550 Turn on HeLa, HepG and HCT-116 + β-Gal (1 U) cell lines with high β-Gal activity per se 42 PBS 344/512 Turn on OVCAR-3 43 H2O-THF 99:1 333/495 Turn on (aggregates) OVCAR-3 44 PBS (20 mM, pH 4.6) 420/565 Turn on (aggregates) SKOV-3 45 PBS 488/560 Turn on (Twophoton) SKOV-3 46 PBS 350/↓450↑5 50 Ratiometric (Twophoton) OVCAR-3 47 PBS/DMSO 7:3 (50 mM, pH = 7.4) 460/650 Turn on (aggregates) OVCAR-3 48 PBS (10 mM, pH 7.4) 410/↓580↑6 50 Ratiometric OVCAR-3 49 PBS/DMSO 99/1 (0.05 M, pH 7.4)

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Section: Coormentioning

confidence: 99%

Chromo-fluorogenic probes for β-galactosidase detection

et al. 2021

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“…These include through-bond energy transfer (TBET), 59,60 recognition between the host and guest, coupled with a specic signal transduction mechanism induced by static quenching, 61 the inner lter effect (IFE), 62 and ICT-FRET. 63 Lin et al designed a coumarin-rhodamine TBET Box, in which the acceptor and donor units are connected by a conjugated spacer. 60 The conguration allows large stokes and emission shis, minimizing constraints on the strong spectral overlaps between acceptor absorption and donor emission.…”

Section: Design Strategies Using Other Mechanismsmentioning

confidence: 99%

“…These include through-bond energy transfer (TBET), 59,60 recognition between the host and guest, coupled with a specific signal transduction mechanism induced by static quenching, 61 the inner filter effect (IFE), 62 and ICT-FRET. 63…”

Section: β-Galactosidase Sensing Mechanismsmentioning

confidence: 99%

“…5d ). 63 The CG probe can also be used to image in both overexpressing cell lines and those with transient expression, showcasing its versatility. This probe also demonstrates excellent selectivity for β-galactosidase in vitro and has the potential to become an ultra-sensitive tool for better understanding physiological and pathological processes in living organisms.…”

Section: β-Galactosidase Sensing Mechanismsmentioning

confidence: 99%

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Design strategies and biological applications of β-galactosidase fluorescent sensor in ovarian cancer research and beyond

Li,

Jia,

et al. 2024

RSC Adv.

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“…Hence, this developing process of the fluorescence sensor minimizes the complex functionalization of the probe. Additionally, the lifetime of UCNPs will not be changed in the process of IFE because there is no new compound formed (Xie et al., 2020). A number of UCNPs‐based sensors for trace elements detection have been fabricated on the basis of the principle of IFE (Fang et al., 2016; Wu et al., 2018; Yang et al., 2019).…”

Section: Rational Design Strategy To Construct Upconversion‐based Sen...mentioning

confidence: 99%

Lanthanide ion (Ln³⁺)‐based upconversion sensor for quantification of food contaminants: A review

Rong

Hassan

Ouyang

et al. 2021

Comp Rev Food Sci Food Safe

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The food safety issue has gradually become the focus of attention in modern society. The presence of food contaminants poses a threat to human health and there are a number of interesting researches on the detection of food contaminants. Upconversion nanoparticles (UCNPs) are superior to other fluorescence materials, considering the benefits of large anti‐Stokes shifts, high chemical stability, non‐autofluorescence, good light penetration ability, and low toxicity. These properties render UCNPs promising candidates as luminescent labels in biodetection, which provides opportunities as a sensitive, accurate, and rapid detection method. This paper intended to review the research progress of food contaminants detection by UCNPs‐based sensors. We have proposed the key criteria for UCNPs in the detection of food contaminants. Additionally, it highlighted the construction process of the UCNPs‐based sensors, which includes the synthesis and modification of UCNPs, selection of the recognition elements, and consideration of the detection principle. Moreover, six kinds of food contaminants detected by UCNPs technology in the past 5 years have been summarized and discussed fairly. Last but not least, it is outlined that UCNPs have great potential to be applied in food safety detection and threw new insight into the challenges ahead.

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Facile and label-free fluorescence sensing of β-galactosidase activity by graphene quantum dots

Cited by 8 publications

References 41 publications

Chromo-fluorogenic probes for β-galactosidase detection

Chromo-fluorogenic probes for β-galactosidase detection

Design strategies and biological applications of β-galactosidase fluorescent sensor in ovarian cancer research and beyond

Lanthanide ion (Ln³⁺)‐based upconversion sensor for quantification of food contaminants: A review

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Facile and label-free fluorescence sensing of β-galactosidase activity by graphene quantum dots

Cited by 8 publications

References 41 publications

Chromo-fluorogenic probes for β-galactosidase detection

Chromo-fluorogenic probes for β-galactosidase detection

Design strategies and biological applications of β-galactosidase fluorescent sensor in ovarian cancer research and beyond

Lanthanide ion (Ln3+)‐based upconversion sensor for quantification of food contaminants: A review

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Lanthanide ion (Ln³⁺)‐based upconversion sensor for quantification of food contaminants: A review