Design strategies and biological applications of β-galactosidase fluorescent sensor in ovarian cancer research and beyond

Li, Liangliang; Jia, Feifei; Li, Yunxiu; Peng, Yan

doi:10.1039/d3ra07968f

Cited by 3 publications

(1 citation statement)

References 113 publications

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“…Its activity is commonly exploited in senescence-associated β-galactosidase staining, which is a hallmark of cellular aging and is used extensively to identify senescent cells in culture and in tissue samples. The detection of β-Gal activity is not only important for understanding basic biological processes but also has implications for diagnosing and tracking diseases, particularly those related to aging like neurodegenerative diseases, cancer, and conditions leading to tissue degeneration [ 222 , 223 , 224 ]. Chai et al developed a novel photochromic fluorescent probe named NpG, specifically engineered for targeting and visualizing β-Gal activity at super-resolution levels within biological samples [ 225 ].…”

Section: Applications Of Photoswitchable Fluorescent and Colorimetric...mentioning

confidence: 99%

Recent Advances in Photoswitchable Fluorescent and Colorimetric Probes

Chen,

Tang,

Yang

et al. 2024

Molecules

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In recent years, significant advancements have been made in the research of photoswitchable probes. These probes undergo reversible structural and electronic changes upon light exposure, thus exhibiting vast potential in molecular detection, biological imaging, material science, and information storage. Through precisely engineered molecular structures, the photoswitchable probes can toggle between “on” and “off” states at specific wavelengths, enabling highly sensitive and selective detection of targeted analytes. This review systematically presents photoswitchable fluorescent and colorimetric probes built on various molecular photoswitches, primarily focusing on the types involving photoswitching in their detection and/or signal response processes. It begins with an analysis of various molecular photoswitches, including their photophysical properties, photoisomerization and photochromic mechanisms, and fundamental design concepts for constructing photoswitchable probes. The article then elaborates on the applications of these probes in detecting diverse targets, including cations, anions, small molecules, and biomacromolecules. Finally, it offers perspectives on the current state and future development of photoswitchable probes. This review aims to provide a clear introduction for researchers in the field and guidance for the design and application of new, efficient fluorescent and colorimetric probes.

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Section: Applications Of Photoswitchable Fluorescent and Colorimetric...mentioning

confidence: 99%

Recent Advances in Photoswitchable Fluorescent and Colorimetric Probes

Chen,

Tang,

Yang

et al. 2024

Molecules

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Dual-responsive near-infrared turn-on fluorescent probe for cancer stem cell-specific visualization

Miki,

Oe,

Suzuki

et al. 2024

J. Mater. Chem. B

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β‐Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine

Zhou,

Liu,

Gao

et al. 2024

Int J of Dairy Tech

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β‐Galactosidases are crucial enzymes that hydrolyse oligosaccharides and polysaccharides with terminal β‐1,4‐glycosidic bonds. Though the traditional application of β‐Galactosidases has been to catalyse the breakdown of lactose in dairy products, its application extends beyond the production of lactose‐free products since variants capable of facilitating lactose condensation and exhibiting galactosyl transferase activity are extensively utilised for the synthesis of prebiotic galacto‐oligosaccharides. This review analyses β‐Galactosidase in multiple aspects, including sources, classification, characterisation, immobilisation, genetic engineering and applications in terms of whey treatment, biofuel production, production of lactose‐free dietary product, synthesis of galacto‐oligosaccharides and the early detection of cellular senescence and tumours.

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Design strategies and biological applications of β-galactosidase fluorescent sensor in ovarian cancer research and beyond

Abstract: Beta-galactosidase (β-galactosidase), a lysosomal hydrolytic enzyme, plays a critical role in the catalytic hydrolysis of glycosidic bonds, leading to the conversion of lactose into galactose.

Cited by 3 publications

References 113 publications

Recent Advances in Photoswitchable Fluorescent and Colorimetric Probes

Recent Advances in Photoswitchable Fluorescent and Colorimetric Probes

Dual-responsive near-infrared turn-on fluorescent probe for cancer stem cell-specific visualization

β‐Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine

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