Facet-Dependent Bactericidal Activity of Ag₃PO₄ Nanostructures against Gram-Positive/Negative Bacteria

Abstract

Ag 3 PO 4 nanostructures (APNs) containing silver (Ag metal; of the noble metal families) have the potential to exhibit enzyme-mimetic activity. A nanostructure shape, including its surface facets, can improve the bioactivity of enzyme mimicry, yet the molecular mechanisms remain unclear. Herein, we report facet-dependent peroxidase and oxidase-like activity of APNs with both antibacterial and biofilm degrading properties through the generation of reactive oxygen species. Cubic APNs had superior antibacterial effects than rhombic dodecahedral shapes when inhibiting Gram-positive and Gram-negative bacterial pathogen proliferation and biofilm degradation. A similar performance was observed for rhombic dodecahedral shapes, being greater than tetrahedral-shaped APNs. The extent of enzyme-mimetic activity is attributed to the facets {100} present in cubic APNs that led the peroxide radicals to inhibit the proliferation of bacteria and degrade biofilm. These facets were compared to rhombic dodecahedral APNs {110} and tetrahedral APNs {111}, respectively, to reveal a facet-dependent enhanced antibacterial activity, providing a plausible mechanism for shape-dependent APNs material enzyme-mimetic effects on bacteria. Thus, our research findings can provide a direction to optimize bactericidal materials using APNs in clinically relevant applications.

Solvent Effect on the Structural, Optical, Morphology, and Antimicrobial Activity of Silver Phosphate Microcrystals by Conventional Hydrothermal Method

“… 18 , 19 Once in an aqueous medium, these radicals attack the organic chains of organic molecules or bacterial walls under low selectivity, resulting in the mineralization of high-weight molecules or inactivation of the basic functions of microorganism cells. 20 − 23 The use of these compounds in reducing cancer cells has also been reported in the literature, 22 becoming an innovative approach in the nonconventional treatment of tumor cells without radiopharmaceuticals or chemotherapy treatments.…”

“…Therefore, the use of advanced oxidative processes (AOPs), which use species with oxidative potential, including hydroxyl radicals (HO • ), superoxides (O 2 •– ), and holes ( h + ), photogenerated in semiconductor materials in aqueous medium has been investigated around the past decades. , Once in an aqueous medium, these radicals attack the organic chains of organic molecules or bacterial walls under low selectivity, resulting in the mineralization of high-weight molecules or inactivation of the basic functions of microorganism cells. − The use of these compounds in reducing cancer cells has also been reported in the literature, becoming an innovative approach in the nonconventional treatment of tumor cells without radiopharmaceuticals or chemotherapy treatments.…”

Unveiling the role of inorganic nanoparticles in Earth’s biochemical evolution through electron transfer dynamics

“…Metal ion architecture in nanoparticles significantly influences inorganic oxidoreductase activities, particularly the POD activity of iron oxide nanoparticles. 49 , 156 , 157 , 158 , 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 Fe 3 O 4 nanoparticles demonstrate higher POD activity compared to γ-Fe 2 O 3 and α-Fe 2 O 3 nanoparticles. 169 Notably, cycling catalysts can transform Fe 3 O 4 to γ-Fe 2 O 3 , reducing ET rates and subsequently lowering POD activity.…”

Inorganic Fe-O and Fe-S oxidoreductases: paradigms for prebiotic chemistry and the evolution of enzymatic activity in biology

“…In addition to size, shape and surface area, recent data indicate that the metal architecture of nanocolloids, including iron oxides, plays a crucial role in enzyme-like activities associated with ET functions ( Liu et al, 2011 ; Puvvada et al, 2012 ; Cheng et al, 2014 ; Mu et al, 2014 ; Peng et al, 2015 ; Ghosh et al, 2018 ; Xu Z. et al, 2021 ; Zhang R. et al, 2021 ; Jiang et al, 2021 ; Chen et al, 2022 ; Singh et al, 2022 ; Zhang et al, 2022 ). In general, the metal architecture of iron oxides is determined by their ferric-ferrous composition ( e.g ., Fe 3+ /Fe total) and the hydroxylation ratio (OH/Fe total), as illustrated in Figure 2A ( Cornell and Schwertmann, 2003 ; Jolivet et al, 2006 ; Jolivet, 2019 ).…”