Fabrication of submicron proteinaceous structures by direct laser writing

Serien, Daniela; Takeuchi, Shoji

doi:10.1063/1.4926659

Cited by 23 publications

(38 citation statements)

References 27 publications

(40 reference statements)

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“…Several studies involving low photoinitiator concentrations have demonstrated that the threshold protein concentration required for fabrication increases dramatically 32,33,68 . Fabrication without photoinitiators has also been reported 32,33,49,68 , although some attempts were unsuccessful 48 . Such discrepancies in prior works indicate that controlling all the major factors such as laser properties, irradiation conditions, solvent, photoactivator and protein concentration that can affect the outcome of the process is the key to achieving repeatability.…”

Section: Photoactivator Systemsmentioning

confidence: 99%

“…Such discrepancies in prior works indicate that controlling all the major factors such as laser properties, irradiation conditions, solvent, photoactivator and protein concentration that can affect the outcome of the process is the key to achieving repeatability. 33 . Phragma dialysis can increase protein concentrations, but can only be applied for several repetitions due to the physical take-off volume limit.…”

Section: Photoactivator Systemsmentioning

confidence: 99%

“…The circular inset shows an SEM image of an icosahedron with submicron feature sizes, scale bar represents 1 μm, reprinted from ref. 33 , with the permission of AIP Publishing, copyright 2015. 37 .…”

Section: Solventsmentioning

confidence: 99%

“…The non-invasive nature of forming crosslinked proteins in solution enables the fabrication of various complex embedded 3D structures. These structures can be nested on the substrate surfaces 33,34 or embedded into transparent devices such as microfluidic channels [35][36][37] . In addition to 3D fabrication and superior resolution, another advantage of the multi-photon cross-linking of proteins as 3D printing materials is the potential to retain the protein functions after fabrication 35,38,39 .…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Serien¹,

Sugioka²

2018

Opto-Electronic Advances

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Proteins are a class of biomaterials having a vast array of functions, including the catalysis of metabolic reactions, DNA replication, stimuli response and transportation of molecules. Recent progress in laser-based fabrication technologies has enabled the formation of three-dimensional (3D) proteinaceous micro-and nano-structures by femtosecond laser cross-linking, which has expanded the possible applications of proteins. This article reviews the current knowledge and recent advancements in the femtosecond laser cross-linking of proteins. An overview of previous studies related to fabrication using a variety of proteins and detailed discussions of the associated mechanisms are provided. In addition, advances and applications utilizing specific protein functions are introduced. This review thus provides a valuable summary of the 3D micro-and nano-fabrication of proteins for biological and medical applications.

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Section: Photoactivator Systemsmentioning

confidence: 99%

Section: Photoactivator Systemsmentioning

confidence: 99%

Section: Solventsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Serien¹,

Sugioka²

2018

Opto-Electronic Advances

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“…Protein molecules in aqueous solution can be cross-linked to construct 3D proteinaceous microstructures by the exposure of a focused femtosecond laser along its trajectory in well-resolved and controlled manners [7][8][9]. Such microstructures made of protein have been applied for cell culture scaffolds [10,11], pH-actuators [9,12,13] and optical elements [13,14].…”

Section: Introductionmentioning

confidence: 99%

Femtosecond Laser Direct Write Integration of Multi-Protein Patterns and 3D Microstructures into 3D Glass Microfluidic Devices

et al. 2018

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Microfluidic devices and biochips offer miniaturized laboratories for the separation, reaction, and analysis of biochemical materials with high sensitivity and low reagent consumption. The integration of functional or biomimetic elements further functionalizes microfluidic devices for more complex biological studies. The recently proposed ship-in-a-bottle integration based on laser direct writing allows the construction of microcomponents made of photosensitive polymer inside closed microfluidic structures. Here, we expand this technology to integrate proteinaceous two-dimensional (2D) and three-dimensional (3D) microstructures with the aid of photo-induced cross-linking into glass microchannels. The concept is demonstrated with bovine serum albumin and enhanced green fluorescent protein, each mixed with photoinitiator (Sodium 4-[2-(4-Morpholino) benzoyl-2-dimethylamino] butylbenzenesulfonate). Unlike the polymer integration, fabrication over the entire channel cross-section is challenging. Two proteins are integrated into the same channel to demonstrate multi-protein patterning. Using 50% w/w glycerol solvent instead of 100% water achieves almost the same fabrication resolution for in-channel fabrication as on-surface fabrication due to the improved refractive index matching, enabling the fabrication of 3D microstructures. A glycerol-water solvent also reduces the risk of drying samples. We believe this technology can integrate diverse proteins to contribute to the versatility of microfluidics.

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Design and Realization of 3D Printed AFM Probes

Alsharif

Burkatovsky

Lissandrello

et al. 2018

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Atomic force microscope (AFM) probes and AFM imaging by extension are the product of exceptionally refined silicon micromachining, but are also restricted by the limitations of these fabrication techniques. Here, the nanoscale additive manufacturing technique direct laser writing is explored as a method to print monolithic cantilevered probes for AFM. Not only are 3D printed probes found to function effectively for AFM, but they also confer several advantages, most notably the ability to image in intermittent contact mode with a bandwidth approximately ten times larger than analogous silicon probes. In addition, the arbitrary structural control afforded by 3D printing is found to enable programming the modal structure of the probe, a capability that can be useful in the context of resonantly amplifying nonlinear tip-sample interactions. Collectively, these results show that 3D printed probes complement those produced using conventional silicon micromachining and open the door to new imaging techniques.

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Fabrication of submicron proteinaceous structures by direct laser writing

Cited by 23 publications

References 27 publications

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Fabrication of three-dimensional proteinaceous micro- and nano-structures by femtosecond laser cross-linking

Femtosecond Laser Direct Write Integration of Multi-Protein Patterns and 3D Microstructures into 3D Glass Microfluidic Devices

Design and Realization of 3D Printed AFM Probes

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