Fabrication of strong, bioactive vascular grafts with PCL/collagen and PCL/silica bilayers for small-diameter vascular applications

Park, Suhyung; Kim, Jin‐Young; Lee, Min-Kyu; Park, Cheonil; Jung, Hyun‐Do; Kim, Hyoun‐Ee; Jang, Tae‐Sik

doi:10.1016/j.matdes.2019.108079

Cited by 93 publications

(57 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrospinning is a widely employed technique to obtain biodegradable grafts (Park et al, 2019), which has been in some cases combined with surface functionalization to promote endothelialization and improve hemocompatibility (Zhao et al, 2019). However, electrospun scaffolds typically suffer from poor cellular infiltration because of the dense fibrous network (Zhong et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering

Fernández‐Colino

Wolf

Rütten

et al. 2019

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Vascular disease is a leading cause of death worldwide, but surgical options are restricted by the limited availability of autologous vessels, and the suboptimal performance of prosthetic vascular grafts. This is especially evident for coronary artery by-pass grafts, whose small caliber is associated with a high occlusion propensity. Despite the potential of tissue-engineered grafts, compliance mismatch, dilatation, thrombus formation, and the lack of functional elastin are still major limitations leading to graft failure. This calls for advanced materials and fabrication schemes to achieve improved control on the grafts' properties and performance. Here, bioinspired materials and technical textile components are combined to create biohybrid cell-free implants for endogenous tissue regeneration. Clickable elastin-like recombinamers are processed to form an open macroporous 3D architecture to favor cell ingrowth, while being endowed with the non-thrombogenicity and the elastic behavior of the native elastin. The textile components (i.e., warp-knitted and electrospun meshes) are designed to confer suture retention, long-term structural stability, burst strength, and compliance. Notably, by controlling the electrospun layer's thickness, the compliance can be modulated over a wide range of values encompassing those of native vessels. The grafts support cell ingrowth, extracellular matrix deposition and endothelium development in vitro. Overall, the fabrication strategy results in promising off-the-shelf hemocompatible vascular implants for in situ tissue engineering by addressing the known limitations of bioartificial vessel substitutes.

show abstract

Section: Introductionmentioning

confidence: 99%

Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering

Fernández‐Colino

Wolf

Rütten

et al. 2019

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…In the literature, different approaches can be found to reach this goal. Often a high-speed rotating collector is used [21][22][23], but fiber orientation can also be reached by using a pair of blades as the collector [24,25] or stretching the electrospun mat in ethanol [2].…”

Section: Introductionmentioning

confidence: 99%

Orientation of Electrospun Magnetic Nanofibers Near Conductive Areas

et al. 2019

View full text Add to dashboard Cite

Electrospinning can be used to create nanofibers from diverse polymers in which also other materials can be embedded. Inclusion of magnetic nanoparticles, for example, results in preparation of magnetic nanofibers which are usually isotropically distributed on the substrate. One method to create a preferred direction is using a spinning cylinder as the substrate, which is not always possible, especially in commercial electrospinning machines. Here, another simple technique to partly align magnetic nanofibers is investigated. Since electrospinning works in a strong electric field and the fibers thus carry charges when landing on the substrate, using partly conductive substrates leads to a current flow through the conductive parts of the substrate which, according to Ampère’s right-hand grip rule, creates a magnetic field around it. We observed that this magnetic field, on the other hand, can partly align magnetic nanofibers perpendicular to the borders of the current flow conductor. We report on the first observations of electrospinning magnetic nanofibers on partly conductive substrates with some of the conductive areas additionally being grounded, resulting in partly oriented magnetic nanofibers.

show abstract

“…But accumulation of some basic properties of natural blood vessel such as rapid endothelialization, mechanical strength, biocompatibility, and anti-thrombosis is quite a big challenge for small diameter (<6 mm) artificial blood vessels. 3 In order to design advanced grafts, decellularized vessel or patient-derived autologous cells have garnered interest because of their potential to minimize graft rejection. However, primary cell isolation and expansion to a therapeutically relevant cells are quite expensive and time-consuming process which could be inactive in case of patients suffering from advanced arterial disease i.e.…”

Section: Introductionmentioning

confidence: 99%

“…13 On the other hand, most vascular studies have focused on compositional features with respect to biological aspects, which cannot satisfy the need of mechanical strength required for practical settings. 3 Hereof, polyurethane polycaprolactone (PUPCL) is another elastic and biocompatible polymer which is useful to construct artificial vascular grafts due to excellent mechanical properties. 14,15 Electrospinning has been employed in a number of tissue applications fields including vascular, neural, bone, cartilage, tendon/ligament, and so on as these constructs can provide a biomimetic environment with a nano-and micro-scale structure.…”

Section: Introductionmentioning

confidence: 99%

“…17 Hence, various types of natural and synthetic polymers have been electrospunned into nano and micro-fibers to fabricate vascular graft within very short preparation time. 3 The aim of this study was to design a small diameter multilayered PCL-MPEG/PUPCL blood vessel by electrospinning technique. To find out a suitable membrane as inner layer with ensuring endothelialization, different PCL-MPEG electrospun fibrous membranes varying MPEG concentrations (0, 5, 10, 20, and 30% w/v) were prepared and analyzed through physicochemical, mechanical, and in vitro biological evaluations especially endothelial markers expression.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro endothelial differentiation evaluation on polycaprolactone-methoxy polyethylene glycol electrospun membrane and fabrication of multilayered small-diameter hybrid vascular graft

et al. 2020

View full text Add to dashboard Cite

During development of functional tissue engineered vascular graft, ensuring endothelialization at inner layer and mechanically strong outer layer is considered as challenging. The study objective was to fabricate a multilayered artificial blood vessel by dual syringe electrospinning technique. The presented graft was composed of polycaprolactone-methoxy polyethylene glycol which acts as an inner layer whereas Poly [4,4'-methylenebis(phenyl isocyanate)-alt-1,4butanediol/di(propylene glycol)/polycaprolactone] (PUPCL) (12% w/v) was incorporated as the outer layer. To find out the optimum inner layer, different polycaprolactone-methoxy polyethylene glycol electrospun membranes varying methoxy polyethylene glycol content (0, 5, 10, 20, and 30% w/v) were prepared and evaluated. The characterizations revealed that blending of methoxy polyethylene glycol with polycaprolactone improved hydrophilicity and antithrombogenic properties compared to polycaprolactone or polyurethane polycaprolactone membrane. During in vitro assessment, the optimized polycaprolactone-20% methoxy polyethylene glycol membrane reinforced a greater differentiation of rat bone marrow stem cell into endothelial cells at both mRNA and protein levels by significantly inducing endothelial markers (CD31, Flk-1, and von Willebrand factor) expression. The polyurethane polycaprolactone membrane showed higher mechanical strength than polycaprolactone-methoxy polyethylene glycol while multilayered polycaprolactone-20% methoxy polyethylene glycol/polyurethane polycaprolactone vascular graft exhibited better result compared to single form. Therefore, the strong and bioactive polycaprolactone-20% methoxy polyethylene glycol/polyurethane polycaprolactone small diameter graft (<2 mm) prepared by simple fabrication process could be a promising preliminary platform for vascular tissue engineering application studies.

show abstract

Fabrication of strong, bioactive vascular grafts with PCL/collagen and PCL/silica bilayers for small-diameter vascular applications

Cited by 93 publications

References 44 publications

Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering

Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering

Orientation of Electrospun Magnetic Nanofibers Near Conductive Areas

In vitro endothelial differentiation evaluation on polycaprolactone-methoxy polyethylene glycol electrospun membrane and fabrication of multilayered small-diameter hybrid vascular graft

Contact Info

Product

Resources

About