Fabrication of Si-based planar type patch clamp biosensor using silicon on insulator substrate

Zhang, Z.L.; Asano, Takao; Uno, Hidetaka; Tero, Ryugo; Suzui, Mitsukazu; Nakao, Shin-ichi; Kaito, Takashi; Shibasaki, Kazuo; Tominaga, Makoto; Utsumi, Yuichi; Gao, Yongli; Urisu, Tsuneo

doi:10.1016/j.tsf.2007.04.104

Cited by 22 publications

(14 citation statements)

References 8 publications

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“…Taken together, our previous results [14] were the building blocks of the present systematic study that provides additional and refined information in Functional high throughput screening technologies have enabled to increase the number of assays on ion channels, now fully exploited as drug targets [19,20,27]. The planar patch-clamp principle is at present used mainly for automated and parallelized whole-cell patch-clamp recordings as evidenced by a considerable number of publications from academic 25 [9,12,14,15,27,33,[34][35][36][37] as well as industrial applications [1,38]. Besides scaling-up the recordings, being able to design and manufacture specific low capacitive chips for example, only by modifying a defined parameter, could be useful for such applications as drug screening.…”

Section: Discussionmentioning

confidence: 67%

“…Since our membrane is SiO 2 -made, our microfabrication process allowed the design of a thicker TEOS SiO 2 membrane, a feature more difficult to obtain with silicon bulk-made membranes [9]. The value of 30 pF is in the best range of those already published for silicon chips [32], except for silicon-on-insulator (SOI) substrate that provides lower noise current [33]. Our low capacitance allowed us to measure efficiently Na + current, known to present very fast activation kinetics (0.8 ms).…”

Section: Discussionmentioning

confidence: 86%

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The development of high quality seals for silicon patch-clamp chips

Sordel¹,

Kermarrec²,

Sinquin³

et al. 2010

Biomaterials

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Planar patch-clamp is a two-dimensional variation of traditional patch-clamp. By contrast to classical glass micropipette, the seal quality of silicon patch-clamp chips (i.e. seal resistance and seal success rate) have remained poor due to the planar geometry and the nature of the substrate and thus partially obliterate the advantages related to planar patch-clamp. The characterization of physical parameters involved in seal formation is thus of major interest. In this paper, we demonstrate that the physical characterization of surfaces by a set of techniques (Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), X-ray Photoelectron Spectroscopy (XPS), surface energy (polar and dispersive contributions), drop angles, impedance spectroscopy, combined with a statistical design of experiments (DOE)) allowed us discriminating chips that provide relevant performances for planar patch-clamp analysis. Analyses of seal quality demonstrate that dispersive interactions and micropore size are the most crucial physical parameters of chip surfaces, by contrast to surface roughness and dielectric membrane thickness. This multi-scale study combined with electrophysiological validation of chips on a diverse set of cell-types expressing various ion channels (IRK1, hERG and hNa(v)1.5 channels) unveiled a suitable patch-clamp chip candidate. This original approach may inspire novel strategies for selecting appropriate surface parameters dedicated to biochips.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 86%

The development of high quality seals for silicon patch-clamp chips

Sordel¹,

Kermarrec²,

Sinquin³

et al. 2010

Biomaterials

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“…The differential conductance of silicon nano-channels can be tuned for optimum performance using the source drain bias voltage, and is sensitive to urea at low concentration. In another work, SOI substrate is used to fabricate the planar type patch clamp ion-channel biosensor, which is suitable for the high throughput screening [39]. The channel current showing the desensitization unique to TRPV1 is measured successfully.…”

Section: Other Materials Used In Biosensormentioning

confidence: 99%

Advances of Nanotechnology Applied to Biosensors

Chen¹,

Dai²,

Cui³

2011

Nano BioMed ENG

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Up to date, application of nanomaterials and nanotechnology has made great advances. Many novel nanomaterials with unique properties are increasingly being exploited to apply for biosensors, improving the property of biosensor and making them higher selectivity and sensitivity, less response time and lower detective limitation. Here we review some of the main advances in this field over the past few years, explore the application prospects, and discuss the issues, approaches, and challenges, with the amin of promting to develop nanomaterials-based biosensor nanotechnology and improving their application in disease diagnosis and biosafety examination.

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“…The pioneering studies of ionchannel biosensors were performed usingpipette patch clamptechniques [1][2][3]. The recently developed planar patch clamp offers the possibility of developing high-throughput screening applications [4][5][6][7][8][9][10]. The planar patch clamp methodhas several advantages over the classical pipette patch clamp in a high-throughput recording system: the former requires less equipment, is insensitive to mechanical vibrations,is capable of collecting long-term measurements, and is relatively easy to use.…”

Section: Introductionmentioning

confidence: 99%

Improvements in the performance of an incubation-type planar patch clamp biosensor using a salt bridge electrode and a plastic (PMMA) substrate

Uno

Wang

Nagaoka

et al. 2014

Sensors and Actuators B: Chemical

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Incubation-type planar patch clamp biosensors, first described in 2008, are expected to enable the realization of high-throughput neural network screening. Several technical challenges must be overcome before this technique may be implemented. First, it is difficult to achieve a sufficiently high seal resistance (a gigaohm seal), which is an important parameter for patch clamp operation. A low seal resistance produces large baseline fluctuations corresponding to the fluctuation of the solid-liquid and liquid-liquid interface potentials.Second, the sensor cell must be positioned at a micropore over a long incubation time while maintaining the cell viability. Finally, a low-cost high-performance sensor chip suitable for high-throughput screening must be developed. These problems have been resolved to some extent in the present work. A stable electrode with a salt bridge structure was developed here to reduce the baseline fluctuations. The cell positioning technology was improved by forming a novel cell trapping pattern using hot embossing on a plastic (polymethylmethacrylate (PMMA)) substrate for use in a microfluidic sensor chip.The combination of the stable electrode and the cell trapping pattern dramatically increased the device success probability from ~2% to 60-80% using HEK293 cells as a model system.The utility of the present results was tested by characterizing rat hippocampal neuron networks. The seal resistance wasfound to be lower than the seal resistance obtainedfrom the HEK293 cells. The effectiveness of the stable electrodes, however, was significantly clear and spontaneous channel currents from the neural network were successfully observed.

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Fabrication of Si-based planar type patch clamp biosensor using silicon on insulator substrate

Cited by 22 publications

References 8 publications

The development of high quality seals for silicon patch-clamp chips

The development of high quality seals for silicon patch-clamp chips

Advances of Nanotechnology Applied to Biosensors

Improvements in the performance of an incubation-type planar patch clamp biosensor using a salt bridge electrode and a plastic (PMMA) substrate

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