Fabrication of shapeless scaffolds reinforced with baghdadite-magnetite nanoparticles using a 3D printer and freeze-drying technique

Karimi, Mehdi; Asefnejad, Azadeh; Aflaki, Dorsa; Aravindhan, Surendar; Baharifar, Hadi; Saber‐Samandari, Saeed; Khandan, Amirsalar; Khan, Afrasyab; Toghraie, Davood

doi:10.1016/j.jmrt.2021.08.084

Cited by 59 publications

(14 citation statements)

References 37 publications

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“…Interestingly, studies have observed that the implantation of a scaffold engineered for BTE purposes at an ectopic site still has the means to recruit bone cells and to generate bone tissue, even if not surrounded by it [ 34 ]. Exquisite studies reported the implantation of BTE-designed scaffolds in other areas (e.g., subcutaneously/in muscle) in order to prove the osteoinductive and osteoconductive properties of the scaffolds [ 35 , 36 , 37 , 38 ]. These unique events have been proven for biomaterials such as β-tricalcium phosphate scaffolds [ 39 ], hydroxyapatite-based materials [ 40 ] and also phosphate graphene composites [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

Graphene–Oxide Porous Biopolymer Hybrids Enhance In Vitro Osteogenic Differentiation and Promote Ectopic Osteogenesis In Vivo

Șelaru

Herman

Vlăsceanu

et al. 2022

IJMS

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Over the years, natural-based scaffolds have presented impressive results for bone tissue engineering (BTE) application. Further, outstanding interactions have been observed during the interaction of graphene oxide (GO)-reinforced biomaterials with both specific cell cultures and injured bone during in vivo experimental conditions. This research hereby addresses the potential of fish gelatin/chitosan (GCs) hybrids reinforced with GO to support in vitro osteogenic differentiation and, further, to investigate its behavior when implanted ectopically. Standard GCs formulation was referenced against genipin (Gp) crosslinked blend and 0.5 wt.% additivated GO composite (GCsGp/GO 0.5 wt.%). Pre-osteoblasts were put in contact with these composites and induced to differentiate in vitro towards mature osteoblasts for 28 days. Specific bone makers were investigated by qPCR and immunolabeling. Next, CD1 mice models were used to assess de novo osteogenic potential by ectopic implantation in the subcutaneous dorsum pocket of the animals. After 4 weeks, alkaline phosphate (ALP) and calcium deposits together with collagen synthesis were investigated by biochemical analysis and histology, respectively. Further, ex vivo materials were studied after surgery regarding biomineralization and morphological changes by means of qualitative and quantitative methods. Furthermore, X-ray diffraction and Fourier-transform infrared spectroscopy underlined the newly fashioned material structuration by virtue of mineralized extracellular matrix. Specific bone markers determination stressed the osteogenic phenotype of the cells populating the material in vitro and successfully differentiated towards mature bone cells. In vivo results of specific histological staining assays highlighted collagen formation and calcium deposits, which were further validated by micro-CT. It was observed that the addition of 0.5 wt.% GO had an overall significant positive effect on both in vitro differentiation and in vivo bone cell recruitment in the subcutaneous region. These data support the GO bioactivity in osteogenesis mechanisms as being self-sufficient to elevate osteoblast differentiation and bone formation in ectopic sites while lacking the most common osteoinductive agents.

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Section: Introductionmentioning

confidence: 99%

Graphene–Oxide Porous Biopolymer Hybrids Enhance In Vitro Osteogenic Differentiation and Promote Ectopic Osteogenesis In Vivo

Șelaru

Herman

Vlăsceanu

et al. 2022

IJMS

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“…The pore size of the biomimetic trachea prepared in this study was about 100 μm with a porosity of about 85%, which met the conditions of most materials for cell growth and differentiation 38,39 ; however, it was still worth improving to obtain more homogeneous‐sized pores. The quantitative comparison of mechanical properties between this study's prepared biomimetic scaffold and rabbit trachea was not possible due to the difference in wall thickness.…”

Section: Discussionmentioning

confidence: 84%

Preparation and evaluation of a silk fibroin–polycaprolactone biodegradable biomimetic tracheal scaffold

Liu

Feng

et al. 2022

J Biomed Mater Res

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In tracheal tissue engineering, the construction of tracheal scaffolds with adequate biodegradable mechanical capacity and biological functions that mimic the structure of a natural trachea is challenging. To explore the feasibility of preparing biomimetic degradable scaffolds with C‐type cartilage rings and an inner tracheal wall of polycaprolactone and silk fibroin. A mold was made according to the diameter of a rabbit trachea, and a silk fibroin tube and polycaprolactone ring attached to the tube were obtained by solution casting. The ring was fixed to the tube at a specific spacing using electrostatic spinning technology to construct a biomimetic tracheal scaffold; its porous structure was observed by scanning electron microscopy, its degradation properties were determined by in vitro enzymatic hydrolysis and its mechanical properties were obtained by pressure testing. The composite scaffold was transplanted subcutaneously into a rabbit model, and the scaffold was taken at 1, 2, and 4 weeks after surgery for sectioning to observe pre‐vascularization. The Medical Ethics Committee of Guangdong Provincial People's Hospital approved the study. The general view of the biomimetic scaffold: the polycaprolactone ring was fixed firmly on the outer wall of the silk fibroin tube; the two corresponded in size, and they fitted closely. The surface of the polycaprolactone ring was smooth and dense, while the surface of the silk fibroin tube could be seen as a uniform porous structure. Scanning electron microscopy showed that the surface and profile of the fibroin tube had a uniform pore size and distribution. The pores were connected to form a network. In vitro, enzymatic hydrolysis experiments confirmed that the fibroin was degraded easily, with most being degraded at the end of week 1. The degradation slowed at 2, 3, and 4 weeks, while the degradation of polycaprolactone was extremely slow. A compression test showed that the compressive resistance of the silk fibroin–polycaprolactone biomimetic scaffolds was much better than that of the rabbit trachea at close thickness. In the tissue staining experiments, as the material degraded, fibrous tissues and blood vessels grew to replace the material, allowing the scaffold to obtain a blood supply and better mechanical properties. A quantitative analysis of CD31 showed that the results for the vascularization of the scaffold were better at 4 weeks than at 2 weeks following subcutaneous grafting (P < .05). The results confirmed that it is feasible to prepare porous, degradable silk fibroin–polycaprolactone biomimetic scaffolds with good mechanical properties and epithelial biological functions by mold casting.

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“…Sublimation and temperature combine to form oriented ice crystals. Despite its benefits, the method is not without disadvantages, including higher energy consumption and long processing times [ 69 ].…”

Section: Pure Baghdadite Ceramicmentioning

confidence: 99%

“… - - - [ 9 ] PCL/Gr/Bagh P19 embryonal carcinoma cells PCL-1Gr-5Bagh scaffold caused further adhesion, viability, and proliferation of the cells, good proliferation of human fibroblast cells on PCL-1Gr-3Bagh and PCL-1Gr-5Bagh nanocomposite scaffolds - - - [ 104 ] PEI/PSS/Bagh/Ag MC3T3-E1 pre-osteoblast dramatically upregulating self-assembly of osteo differentiation markers, including ALP, collagen secretion, and Ca deposition by using baghdadite, promoting ALP expression and ECM (osteogenesis) by releasing Ca, Si and Zn ions, catechol and amine groups of PDA and PEI can exert beneficial osteogenic effects by serving as active recruiting sites of mineral ions and aid in apatite nucleation and growth. - - self-antibacterial coating against Staphylococcus aureus adhesion [ 105 ] Bagh/PCL Saos-2 cells line No cytotoxicity, good attachment and proliferation of cells on the both coated Bagh and Bagh/PCL scaffolds, less cell viability for scaffold with PCL coating in comparison to uncoated Bagh - - - [ 54 ] Bagh/Dio/PCLF Bone marrow stem cells (BMSc) Good attachment, high proliferation, and spreading of the BMSc cells on the surface of Bagh/Dio/PCLF scaffold, good biocompatibility, no toxicity - - - [ 27 ] N6/Bagh MG63 cells Increasing cellular activity, and cell viability, better cell attachment and spreading by increasing the amount of Bagh in composite scaffold in comparison to N6 - - - [ 106 ] PLA/Bagh/Alginate/Fe 3 O 4 - Sample including 20 wt% baghdadite has less toxicity than other samples - - - [ 69 ] Bagh/CN coating Human bone marrow mesenchymal stem cells Enhancing growth, proliferation, adhesion, and viability, activation of Zr ions on the differentiation of cells, bone formation and repair capability - …”

Section: Applications Of Baghdadite Ceramicmentioning

confidence: 99%

Recent advances on bioactive baghdadite ceramic for bone tissue engineering applications: 20 years of research and innovation (a review)

Sadeghzade

Liu²,

Wang³

et al. 2022

Materials Today Bio

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Fabrication of shapeless scaffolds reinforced with baghdadite-magnetite nanoparticles using a 3D printer and freeze-drying technique

Cited by 59 publications

References 37 publications

Graphene–Oxide Porous Biopolymer Hybrids Enhance In Vitro Osteogenic Differentiation and Promote Ectopic Osteogenesis In Vivo

Graphene–Oxide Porous Biopolymer Hybrids Enhance In Vitro Osteogenic Differentiation and Promote Ectopic Osteogenesis In Vivo

Preparation and evaluation of a silk fibroin–polycaprolactone biodegradable biomimetic tracheal scaffold

Recent advances on bioactive baghdadite ceramic for bone tissue engineering applications: 20 years of research and innovation (a review)

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