Fabrication of Pt/Cu₃(PO₄)₂ ultrathin nanosheet heterostructure for photoelectrochemical microRNA sensing using novel G-wire-enhanced strategy

Abstract

Herein, we focus on preparing a highly efficient photocatalytic material to construct a signal-on photoelectrochemical (PEC) sensing platform in view of the rigorous demand of accurate miRNA quantification. The well-dispersed Pt nanoclusters-coated copper phosphate ultrathin nanosheets (PtNCs/Cu(PO)NSs) were first successfully synthesized as a photoelectrode material. Because of the ultrathin two-dimensional lamellar structure of Cu(PO)NSs with a 1.3 nm thickness, as well as the homogeneous size and abundant PtNCs loaded on Cu(PO)NSs, the resultant PtNCs/Cu(PO)NSs were employed as a photoelectrode material for the first time and revealed outstanding photocatalytic activity in PEC sensing as a substrate. As a well-designed protocol, we realized accurate miRNA quantification via a novel signal amplification strategy based on G-wire superstructure exponentially ligating a signal probe, which possesses efficient and simple operation compared to the traditional amplification method. Moreover, the electron donor is generated in situ by lactate oxidase (Lox) labels catalyzing lactate for HO production, boosting the efficient separation of electron-hole pairs for further signal amplification. Impressively, this PEC sensing platform is commendably utilized to determine miRNA-141 from prostate carcinoma cell line 22Rv1. This study, considering the excellent PtNCs/Cu(PO)NSs combined with G-wire superstructure for exponential signal amplification strategy, paves a new path in biosensing and clinical diagnosis.

Novel Enzyme-Free Multifunctional Bentonite/Polypyrrole/Silver Nanocomposite Sensor for Hydrogen Peroxide Detection over a Wide pH Range

“…Hydrogen peroxide (H 2 O 2 ) is important in myriad industrial, environmental remediation, biological, and pharmaceutical applications [1,2,3,4,5,6,7,8]. Moreover, H 2 O 2 is ubiquitously generated as a by-product during cholesterol [9], glucose [10], glutamate [11], and lactate [12] oxidation processes. Numerous efforts are spent to develop efficient analytical methods for the detection of H 2 O 2 , namely, high-pressure liquid chromatography, colorimetric, positron emission tomography, electrochemical, bioluminescence and chemiluminescence [13,14,15,16,17].…”

Hydrogen Production Improvement on Water Decomposition Through Internal Interfacial Charge Transfer in M3(PO4)2-M2P2O7 Mixed-Phase Catalyst (M = Co, Ni, and Cu)

“…In Scheme 1a, the bandgap position of Cu2P2O7 particle does not contain the redox potential for water decomposition, and it has the reduction potential of hydrogen but has not the oxidation potential of oxygen [51]. Eventually, the Scheme 1a mechanism is undesirable in this study, even though the Cu3(PO4)2 includes the redox potential for water decomposition [52]. Thus, like Scheme 1b, excited electrons can only move.…”

Ti3C2 MXene-based Schottky photocathode for enhanced photoelectrochemical sensing

“…Photoelectrochemical (PEC) sensing has been a rapidly evolving analytical technique for trace or ultratrace detection towards diverse biomolecular in complex samples, since the technique is of considerate merits of low background signal, high sensitivity, and simple operating conditions [1][2][3]. Potential photoelectrode candidates should possess highly valid light harvesting, suitable analytes interfacing, efficient PEC signal response, and stable signal transduction to exploit ideal PEC sensing platform [4][5][6]. Up to now, plentiful efforts are focused on developing photoactive materials to obtain desirable photoelectrode, such as TiO 2 -based or quantum dots-based nanomaterials, as well as porphyrin and its derivatives [7,8].…”