Fabrication of PLA/Ag nanofibers by green synthesis method using Momordica charantia fruit extract for wound dressing applications

Alippilakkotte, Shebi; Kumar, Sanjeev; Sreejith, Lisa

doi:10.1016/j.colsurfa.2017.06.066

Cited by 99 publications

(62 citation statements)

References 40 publications

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“…Figure S3A,B show the FTIR spectra of the PLA/PEG nanofilms without and with entrapped AgNPs, respectively. It can be found that the both spectrums have almost the same absorption peaks which are perfectly matched with the previous PLA/PEG FTIR reports 45,66,67 . At the same time, several slight shifts for the entrapped AgNPs sample are related to the interaction of Ag NPs with the PLA/PEG composite.…”

Section: Morphology Of Agnps Containing Nanofilmssupporting

confidence: 85%

“…At the same time, several slight shifts for the entrapped AgNPs sample are related to the interaction of Ag NPs with the PLA/PEG composite. In detail, the shift of the C-O stretching frequency from 1084.5 cm-1 (for PEG-PLA sample) to 1087 cm-1 (for PEG-PLA@Ag sample), and the strong decrement in the intensity of the -CH2 stretching frequency of the PEG-PLA@Ag sample (in comparison with that of PEG-PLA sample) with its slight shift from 2875.2 cm-1 to 2877.6 cm-1, are attributed to the conjugation of oxygen atoms with the surface of AgNPs 45,67 .…”

Section: Morphology Of Agnps Containing Nanofilmsmentioning

confidence: 98%

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Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Bardania

Mahmoudi

Bagheri

et al. 2020

Sci Rep

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To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. However, the high amount of reactive oxygen species, microbial infection, and damaging extracellular matrix remain as the main challenges for the wound healing process. In this study, for the first time, green synthesized silver nanoparticles (AgNPs) using Teucrium polium extract were embedded in poly lactic acid/poly ethylene glycol (PLA/PEG) film to provide absorbable wound dressing, with antioxidant and antibacterial features. The physicochemical analysis demonstrated, production of AgNPs with size approximately 32.2 nm and confirmed the presence of phytoconstituents on their surface. The antibacterial assessments exhibited a concentration-dependent sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa toward biosynthesized AgNPs, which showed a suitable safety profile in human macrophage cells. Furthermore, oxidant scavenging assays demonstrated exploitation of plant extract as a reducing agent, endows antioxidant activity to biogenic AgNPs. The formation of PLA/PEG nanofilm and entrapment of AgNPs into their matrix were clearly confirmed by scanning electron microscopy. More importantly, antibacterial examination demonstrated that the introduction of biogenic AgNPs into PLA/PEG nanofibers led to complete growth inhibition of P. aeruginosa and S. aureus. In summary, the simultaneous antioxidant activity and antimicrobial activity of the novel biogenic AgNPs/PLA/PEG nanofilm showed its potential for application as wound dressing.activities of porous Ag nanofilm were investigated. We anticipate that the Ag nanofilm wound dressing with free radical scavenging capacity would enhance the wound healing process. Moreover, this study proposed a low-cost and facile method for preparation of Ag nanofilm as a potential wound dressing. Materials and MethodsMaterials. Methanol (CH 3 OH, 99.9%), silver nitrate (AgNO 3 ), nutrient agar, ferric chloride hexahydrate (FeCl 3 , 6H 2 O), iron (II) sulfate tetrahydrate (FeSO 4 .4H 2 O), dichloromethane, polyethylene glycol 300, ascorbic acid and Mueller-Hinton agar (MHA) were purchased from Merck (Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and 2,4,6-Tripyridyl-s-Triazine (TPTZ) were purchased from Sigma (Germany) and poly lactic acid (PLA) from Zhejiang Hisun Biomaterials (China). The RAW264 macrophage cell line was purchased from National Cell Bank, Pasteur Institute of Iran, Iran and Dulbecco's Modified Eagle's Medium (DMEM), Fetal Bovine Serum (FBS), 1% Penicillin-Streptomycin from Gibco (Paisley, UK). All aqueous solutions were prepared using double distilled water. All reagents used were of analytical grade. Clinical strains of Pseudomonas aeruginosa 38 and Staphylococcus aureus 39 bacteria were isolated from hospitalized patients by our group, which have been characterized as antibiotic-resistant strains.Extract preparation. T.polium is a wild-growing...

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Section: Morphology Of Agnps Containing Nanofilmssupporting

confidence: 85%

Section: Morphology Of Agnps Containing Nanofilmsmentioning

confidence: 98%

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Bardania

Mahmoudi

Bagheri

et al. 2020

Sci Rep

View full text Add to dashboard Cite

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“…Some of the prominent examples of these plants are Cassia roxburghii, 66 Drosera binata Labill., 67 Indigofera aspalathoides DC., 68 Azadirachta indica A. Juss., 69 Arnebia nobilis Rech.f., 70 Melia dubia Cav., 71 Terminalia chebula Retz., 72 Lansium domesticum Corrêa, 73 Orchidantha chinensis T.L.Wu, 74 A. vera (L.) Burm.f., 75 glucuronoxylan, 76 Momordica charantia L., 77,78 Carica papaya L., 79 Cymbopogon citratus (DC.) Stapf, 80 Nyctanthes arbor-tristis L., 81 Naringi crenulata (Roxb.)…”

Section: Green-synthesized Metal Nanoparticles Using Plantsmentioning

confidence: 99%

Natural product-based nanomedicines for wound healing purposes: therapeutic targets and drug delivery systems

Hajialyani¹,

Tewari²,

Sobarzo‐Sánchez³

et al. 2018

IJN

166

113

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Wound healing process is an intricate sequence of well-orchestrated biochemical and cellular phenomena to restore the integrity of the skin and subcutaneous tissue. Several plant extracts and their phytoconstituents are known as a promising alternative for wound healing agents due to the presence of diverse active components, ease of access, and their limited side effects. The development of nanotechnological methods can help to improve the efficacy of different therapeutics as well as herbal-based products. Here, we present a review of the efficacy of the plant based-nanomaterials in the management of wounds and discuss the involved therapeutic targets. For this purpose, a profound search has been conducted on in vitro, in vivo, and/or clinical evidences evaluating the efficacy and pharmacological mechanisms of natural product-based nanostructures on different types of wounds. Different pharmacological targets are involved in the wound healing effects of herbal-based nanostructures, including suppressing the production of inflammatory cytokines and inflammatory transduction cascades, reducing oxidative factors and enhancing antioxidative enzymes, and promoting neovascularization and angiogenic pathways through increasing the expression of vascular endothelial growth factor, fibroblast growth factor, and platelet-derived growth factor. Moreover, nanostructure of plant extracts and their phytochemicals can enhance their bioavailability, control their release in the form of sustained delivery systems to the wound site, and enhance the permeability of these therapeutics to the underlying skin layers, which are all necessary for the healing process. Overall, various plant extracts and their natural compounds, used in nanoformulations, have demonstrated high activity in the management of wounds and thus can be assumed as future pharmaceutical drugs.

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“…Previous studies revealed the wound healing activity of bitter melon, and the promotion of wound healing through antiinflammatory and antibacterial effects and the stimulation of angiogenesis and fibroblast activity has been demonstrated in animal studies (Ilango et al, 2010; Prashanthi et al, 2012;Satar et al, 2013;Hussan et al, 2014;Pişkin et al, 2014;İlhan et al 2015;Singh et al 2017). Furthermore, in a different study, it was reported that bitter melon fruit extract-based PLA/Ag nanofibers used as wound healers can enhance the proliferation and function of epidermal cells and fibroblasts (Alippilakkotte et al, 2017). Measurement of the wound area and change in the surface area of the wound provides objective evidence of the wound healing process.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of bitter melon extract in the treatment of ischemic wounds in rats

Kısacık¹,

Güneş²,

Yaprakci³

et al. 2018

Turk J Biol

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There is no consensus on the properties of an ideal dressing for treating wounds. The aim of this study was to investigate the efficacy of dressings using topically administered bitter melon extract with olive oil, pure olive oil, nitrofurazone, and saline in the healing of ischemic wounds. A sample group of 48 rats was used in the trial. Their wounds were treated with bitter melon extract, pure olive oil, nitrofurazone, and saline. Data were collected between October 2014 and April 2015. The highest percentage (94.7%) of wound healing was observed in the bitter melon extract group and the lowest percentage (86.3%) in the nitrofurazone group. At the end of the 21st day, macroscopic reepithelialization was observed in 9 wounds in the bitter melon extract group (75%), in 6 wounds in the pure olive oil group (50%), and in only 3 wounds in the nitrofurazone and saline groups (25%). It can be concluded that dressing with a bitter melon extract is more efficient in the treatment of wounds than using nitrofurazone or saline, and that dressing with olive oil accelerates wound healing, although not as much as dressing with bitter melon extract.

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Fabrication of PLA/Ag nanofibers by green synthesis method using Momordica charantia fruit extract for wound dressing applications

Cited by 99 publications

References 40 publications

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Natural product-based nanomedicines for wound healing purposes: therapeutic targets and drug delivery systems

Effectiveness of bitter melon extract in the treatment of ischemic wounds in rats

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