Fabrication of pillar-like titania nanostructures on titanium and their interactions with human skeletal stem cells

Sjöström, Terje; Dalby, Matthew J.; Hart, Andrew; Tare, Rahul S.; Oreffo, Richard O.C.; Su, Bo

doi:10.1016/j.actbio.2009.01.007

Cited by 242 publications

(210 citation statements)

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“…Enhancement of cell adhesion by various nanotextures or micro/nanotextures has been widely reported. 16,23,[31][32][33] However, the exact effect of nanotextures on cell adhesion activity remains to be determined. 34 One possible explanation is that the high surface energy of the sharp ridges in the nanoscale range facilitates formation of focal adhesions.…”

Section: Cell Adhesion Activitymentioning

confidence: 99%

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Huang³

et al. 2013

IJN

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Background and methods:Various methods have been used to modify titanium implant surfaces with the aim of achieving better osseointegration. In this study, we fabricated a clustered nanorod structure on an acid-etched, microstructured titanium plate surface using hydrogen peroxide. We also evaluated biofunctionalization of the hybrid micro/nanorod topography on rat bone marrow mesenchymal stem cells. Scanning electron microscopy and x-ray diffraction were used to investigate the surface topography and phase composition of the modified titanium plate. Rat bone marrow mesenchymal stem cells were cultured and seeded on the plate. The adhesion ability of the cells was then assayed by cell counting at one, 4, and 24 hours after cell seeding, and expression of adhesion-related protein integrin β1 was detected by immunofluorescence. In addition, a polymerase chain reaction assay, alkaline phosphatase and Alizarin Red S staining assays, and osteopontin and osteocalcin immunofluorescence analyses were used to evaluate the osteogenic differentiation behavior of the cells. Results:The hybrid micro/nanoscale texture formed on the titanium surface enhanced the initial adhesion activity of the rat bone marrow mesenchymal stem cells. Importantly, the hierarchical structure promoted osteogenic differentiation of these cells. Conclusion: This study suggests that a hybrid micro/nanorod topography on a titanium surface fabricated by treatment with hydrogen peroxide followed by acid etching might facilitate osseointegration of a titanium implant in vivo.

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Section: Cell Adhesion Activitymentioning

confidence: 99%

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Huang³

et al. 2013

IJN

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“…However, a small drawback is its bioinert nature [3]. To overcome this, possible strategies are to optimize the topography or to mix it with another material that can activate cell growth [4]. Silicon oxide supports cell growth and hence can be used as the other phase in the mixture [5].…”

Section: Introductionmentioning

confidence: 99%

Open air plasma deposited antimicrobial SiO_x/TiO_x composite films for biomedical applications

Rapp

Baumgärtel

Artmann

et al. 2016

Current Directions in Biomedical Engineering

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Open air atmospheric pressure plasma jet (APPJ) enhanced chemical vapour deposition process was used to deposit biocompatible SiO x /TiO x composite coatings. The as deposited films are hydrophilic and show visible light induced photocatalytic effect, which is a consequence of the formation of defects in the TiO x structure due to the plasma process. This photocatalytic effect was verified by the demonstration of an antimicrobial effect under visible light on E. coli as well as by degradation of Rhodamine B. The films are non-cytotoxic as shown by the cytocompatibility tests. The films are conductive to cell growth and are stable in DMEM and isopropanol. The structural evaluation using SEM, EDS and XPS shows a dispersion of TiO x phase in a SiO x CyHz matrix. These analyses were used to correlate the structure-property relationship of the composite coating.

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“…For example, by creating nano-sized features on titanium-based surfaces the expression of bone sialoprotein (BSP) and osteopontin was stimulated by osteogenic cells (2). The enhancement of osteoblast adhesion and function on different nanostructured metal and ceramic materials was demonstrated in further studies (3,4).…”

Section: Introductionmentioning

confidence: 99%

NCO-sP(EO-stat-PO) surface coatings preserve biochemical properties of RGD peptides

Fiedler

Groll²,

Engelhardt³

et al. 2010

Int J Mol Med

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Abstract.We have previously reported that star shaped poly(ethylene oxide-stat-propylene oxide) macromers with 80% EO content and isocyanate functional groups at the distal ends [NCO-sP(EO-stat-PO)] can be used to generate coatings that are non-adhesive but easily functionalized for specific cell adhesion. In the present study, we investigated whether the NCO-sP(EO-stat-PO) surfaces maintain peptide configuration-specific cell-surface interactions or if differences between dissimilar binding molecules are concealed by the coating. To this end, we have covalently immobilized both linear-RGD peptides (gRGDsc) and cyclic-RGD (RGDfK) peptides in such coatings. Subsequently, SaOS-2 or human multipotent mesenchymal stromal cells (MSC) were seeded on these substrates. Cell adhesion, spreading and survival was observed for up to 30 days. The time span for cell adherence was not different on linear and cyclic RGD peptides, but was shorter in comparison to the unmodified glass surface. MSC proliferation on cyclic RGDfK modified coatings was 4 times higher than on films functionalized by linear gRGDsc sequences, underlining that the NCO-sP(EO-stat-PO) film preserves the configuration-specific biochemical peptide properties. Under basal conditions, MSC expressed osteogenic marker genes after 14 days on cyclic RGD peptides, but not on linear RGD peptides or the unmodified glass surfaces.Our results indicate specific effects of these adhesion peptides on MSC biology and show that this coating system is useful for selective testing of cellular interactions with adhesive ligands. IntroductionThe surface design of biomaterials is a key element controlling their integration into the surrounding tissue and cell adhesion (1). The biomaterial surface modulates cellular responses in terms of adhesion, proliferation, inflammatory reactions and survival.Due to its surface properties an artificial implant usually induces the production of a fibrous tissue covering its surface after implantation. This fibrous layer reduces the tissueimplant contact, which may result in loosening of the implant or stimulation of further inflammatory processes. There are several strategies to enhance the ingrowth of non-biological materials by addressing non-specific cell-surface interactions. Alterations of the physical surface properties like hydrophobicity or surface topography are possibilities. For example, by creating nano-sized features on titanium-based surfaces the expression of bone sialoprotein (BSP) and osteopontin was stimulated by osteogenic cells (2). The enhancement of osteoblast adhesion and function on different nanostructured metal and ceramic materials was demonstrated in further studies (3,4).A more biomimetic strategy is to control adhesion, proliferation and differentiation of osteogenic cells by biochemical modification of the implant surface. While coating with hydroxyapatite is often used in combination with an alteration of surface roughness to improve osteointegration of implants (5), this approach does not minimize non-specific...

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Fabrication of pillar-like titania nanostructures on titanium and their interactions with human skeletal stem cells

Cited by 242 publications

References 46 publications

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Open air plasma deposited antimicrobial SiO_x/TiO_x composite films for biomedical applications

NCO-sP(EO-stat-PO) surface coatings preserve biochemical properties of RGD peptides

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Fabrication of pillar-like titania nanostructures on titanium and their interactions with human skeletal stem cells

Cited by 242 publications

References 46 publications

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Open air plasma deposited antimicrobial SiOx/TiOx composite films for biomedical applications

NCO-sP(EO-stat-PO) surface coatings preserve biochemical properties of RGD peptides

Contact Info

Product

Resources

About

Open air plasma deposited antimicrobial SiO_x/TiO_x composite films for biomedical applications