Fabrication of novel high performance ductile poly(lactic acid) nanofiber scaffold coated with poly(vinyl alcohol) for tissue engineering applications

Abdal‐hay, Abdalla; Hussein, Kamal Hany; Casettari, Luca; Khalil, Khalil Abdelrazek; Hamdy, Abdel Salam

doi:10.1016/j.msec.2015.11.024

Cited by 97 publications

(78 citation statements)

References 43 publications

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“…In vivo implantation of a scaffold necessarily implies that it is subjected to mechanical forces; hence, it has to be capable of maintaining a certain structural integrity to provide a more suitable support for regeneration (Abdal‐Hay, Hussein, Casettari, Khalil, & Hamdy, ). Mechanical characteristics of the membrane affect the final results in tissue regeneration and its tensile strength when sutured may influence the clinical outcome of tissue healing.…”

Section: Discussionmentioning

confidence: 99%

Biofabrication of a novel leukocyte-fibrin-platelet membrane as a cells and growth factors delivery platform for tissue engineering applications

Barbon

Stocco

Grandi

et al. 2018

J Tissue Eng Regen Med

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Autologous platelet-rich hemocomponents have emerged as potential biologic tools for regenerative purpose, but their therapeutic efficacy still remains controversial. This work represents the characterization study of an innovative autologous leukocyte-fibrin-platelet membrane (LFPm), which we prepared according to a novel protocol involving multiple cycles of apheresis. The high content in fibrinogen gave to our hemocomponent the appearance of a manipulable and suturable membrane with high elasticity and deformation capacity. Moreover, being highly enriched with platelets, leukocytes, and monocytes/macrophages, the LFPm sustained the local release of bioactive molecules (platelet derived growth factor, vascular endothelial growth factor, interleukin-10, and tumour necrosis factor alpha). In parallel, the evaluation of stemness potential highlighted also that the LFPm contained cells expressing pluripotency and multipotency markers both at the messenger ribonucleic acid (NANOG, SOX2, THY1, NT5E, and ENG) and surface-protein level (CD44 /CD73 /CD34 /CD117 /CD31 ). Finally, biodegradation analysis interestingly showed a good stability of the membrane for at least 3 weeks in vitro and 1 week in vivo. In both cases, biodegradation was associated with progressive exposure of fibrin scaffold, loss/migration of cellular elements, and release of growth factors. Overall, collected evidence could shed some light on the regenerative effect that LFPms may exert after the autologous implant on a defect site.

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Section: Discussionmentioning

confidence: 99%

Biofabrication of a novel leukocyte-fibrin-platelet membrane as a cells and growth factors delivery platform for tissue engineering applications

Barbon

Stocco

Grandi

et al. 2018

J Tissue Eng Regen Med

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“…The observed peaks at 1640 and 3400 cm −1 are the sign of OH in chemical composition. Also, characteristic peaks at 1250, 1100, and 850 cm −1 indicate stretching of the CO. 36,37,47 The FTIR spectra of GPTMS exhibited the characteristic bonds at 1087 and 1201 cm −1 due to CH 2 of glycidoxy and propyl chain group, respectively. The peak at 2900 cm −1 correspond with the CH 2 symmetric stretching, and the peaks at 1380 and 1450 cm −1 are corroborated with CH 2 vibrations.…”

Section: Fourier Transmission Infrared Spectroscopymentioning

confidence: 98%

“…35 In this research, polyvinyl alcohol (PVA) was immobilized on the O 2− plasma-modified surface. Abdal-hay et al 37 coated PVA on the poly(lactic acid) (PLA) nanofibrous scaffolds. It is expected that PVA immobilization introduces abundant hydrophilic functional groups to the surface of the superhydrophobic polymer, and compensates the PU weakness in cellular interactions.…”

Section: Introductionmentioning

confidence: 99%

“…contact angle values of the PC, PCO, PCOP, and PCOPG were measured about 130.3, 61.6, 44.2, and 49.4 , respectively [Figure 5 (a-d)], which investigated that the contact angle decreased after carried out O 2 -plasma, and PVA immobilization gradually. Abdal-hay et al37 showed the improvement in the hydrophilicity of the PVA-coated PLA nanofibrous scaffolds in comparison with the PLA one. These results also are in agreement with the FTIR findings.…”

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confidence: 99%

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Immobilization of polyvinyl alcohol‐siloxane on the oxygen plasma‐modified polyurethane‐carbon nanotube composite matrix

Aidun

Zamanian

Ghorbani

2019

J of Applied Polymer Sci

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Polyurethane-based scaffolds have been considered as a promising strategy for tissue regeneration. Herein, the polyurethane and carbon nanotubes electrospun scaffolds were modified by polyvinyl alcohol-3-glycidoxypropyl-trimethoxysilane after oxygen plasma treatment to improve physicochemical and in vitro properties for efficient bone reconstruction. Finally, the morphology of scaffolds, chemical characterization, surface roughness, bioactivity, hydrophilicity, cell attachment, cell viability, and alkaline phosphatase activity were investigated. According to microscopy results, bead free and smooth fibers were obtained using electrospinning while the degree of uniformity was reduced after the surface modification process. However, the modification process induced higher hydrophilicity and bioactivity to prepared scaffolds. In addition, the attachment and viability of the cells were improved as a function of surface modification. The expression of alkaline phosphatase especially in modified fibers confirmed the initial potential of scaffolds for bone tissue engineering applications and further studies.

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“…Abdalla Abdal-hay et al (2016) [56] fabricated novel high performance ductile PLLA nanofiber scaffold coated with PVA for tissue engineering applications. However, the low hydrophilicity and poor ductility restrict its practical application.…”

Section: Plla Scaffoldsmentioning

confidence: 99%

A Review on Biodegradable Polymeric Materials Striving Towards the Attainment of Green Environment

Jayanth¹,

Kumar

Nayak

et al. 2017

J Polym Environ

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Fabrication of novel high performance ductile poly(lactic acid) nanofiber scaffold coated with poly(vinyl alcohol) for tissue engineering applications

Cited by 97 publications

References 43 publications

Biofabrication of a novel leukocyte-fibrin-platelet membrane as a cells and growth factors delivery platform for tissue engineering applications

Biofabrication of a novel leukocyte-fibrin-platelet membrane as a cells and growth factors delivery platform for tissue engineering applications

Immobilization of polyvinyl alcohol‐siloxane on the oxygen plasma‐modified polyurethane‐carbon nanotube composite matrix

A Review on Biodegradable Polymeric Materials Striving Towards the Attainment of Green Environment

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