Fabrication of novel biofibers by coating silk fibroin with chitosan impregnated with silver nanoparticles

Abstract

Nanoparticle based agents often applied as coatings on biomaterials have shown promise in delivering the improved sterility against variety of microbes. In the present study, silk fibers (SF) were coated with chitosan impregnated with silver nanoparticles (Ag-C-SF). These Ag-C-SF fibers were characterized using scanning electron microscopy (SEM) with energy dispersive X-ray analysis, atomic force microscopy (AFM), Infra Red spectroscopy, Thermogravimetric Analysis and Microbiological assay techniques. AFM studies have confirmed the nano sized silver particles in chitosan solution; SEM pictures have exhibited the coating of chitosan along with silver nanoparticles on the silk fibroin. The modified fibers have also shown anti-microbial activity and improved thermal stability. The Ag-C-SF fibers may be explored as wound dressing and tendon reconstruction material in future.

Nanoparticles for Tendon Healing and Regeneration: Literature Review

“…Karthikeyan et al (2011) developed a system to produce functional biofibers containing silk fibers (SF) coated with chitosan and impregnated with silver nanoparticles (Ag–C–SF). Chitosan [poly-b-(1-4)-D-glucosamine] is a sustainable, biocompatible, biodegradable, and antimicrobial polysaccharide of great relevance in many fields of application.…”

“…Chitosan [poly-b-(1-4)-D-glucosamine] is a sustainable, biocompatible, biodegradable, and antimicrobial polysaccharide of great relevance in many fields of application. They tested the fibers with microbiological assays, Scanning electron microscopy (SEM), infrared spectroscopy, AFM studies and thermo gravimetric analysis (Karthikeyan et al, 2011). The results shown antimicrobial activity (nanoparticles enter into bacteria, inhibit the ATP Synthesis and denature DNA, and blocking the respiratory chain) and increased thermal stability (Karthikeyan et al, 2011).…”

“…They tested the fibers with microbiological assays, Scanning electron microscopy (SEM), infrared spectroscopy, AFM studies and thermo gravimetric analysis (Karthikeyan et al, 2011). The results shown antimicrobial activity (nanoparticles enter into bacteria, inhibit the ATP Synthesis and denature DNA, and blocking the respiratory chain) and increased thermal stability (Karthikeyan et al, 2011). The authors emphasize that this fiber might be a promising material in wound healing and tendon reconstruction (Karthikeyan et al, 2011).…”

“…The results shown antimicrobial activity (nanoparticles enter into bacteria, inhibit the ATP Synthesis and denature DNA, and blocking the respiratory chain) and increased thermal stability (Karthikeyan et al, 2011). The authors emphasize that this fiber might be a promising material in wound healing and tendon reconstruction (Karthikeyan et al, 2011). …”

Applications of nanomaterials in mechano-sensitive tissues

“…Silk fibres impregnated with NPs have shown to improve the sterility against multiple microbes. Silk fibres were coated with CS impregnated with silver NPs (Ag-C-SF), which supported anti-microbial activity and improved thermal stability, showing potential to be explored as a wound dressing and for tendon reconstruction [131].…”