Fabrication of macromolecular gradients in aligned fiber scaffolds using a combination of in-line blending and air-gap electrospinning

Kishan, Alysha; Robbins, Andrew B.; Mohiuddin, Sahar; Jiang, Mingliang; Moreno, Michael R.; Cosgriff‐Hernandez, Elizabeth

doi:10.1016/j.actbio.2016.12.041

Cited by 50 publications

(80 citation statements)

References 48 publications

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“…Furthermore, micropatterned collectors can be designed to obtain aligned porous scaffolds containing both micro/macropores and a nanofibrous structure without requiring additional control or postprocessing steps; [39,83] gradients in both the radial direction and the longitudinal direction can also be directly incorporated into the macroporous hydrogel by designing appropriate mandrel-based collectors. [84,85] The main drawback of electrospinning is the stability of the resulting macroporous networks (particularly under pressure) given that there are typically no bonds between the fibers themselves, making them relatively easy to compress. In addition, while the nanofiber diameter can be precisely controlled, it is much more challenging to control the spacing between the fibers given the random whipping motion that occurs upon solvent evaporation.…”

Section: Electrospinningmentioning

confidence: 99%

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Hoare

2017

Adv Healthcare Materials

155

165

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Structured macroporous hydrogels that have controllable porosities on both the nanoscale and the microscale offer both the swelling and interfacial properties of bulk hydrogels as well as the transport properties of “hard” macroporous materials. While a variety of techniques such as solvent casting, freeze drying, gas foaming, and phase separation have been developed to fabricate structured macroporous hydrogels, the typically weak mechanics and isotropic pore structures achieved as well as the required use of solvent/additives in the preparation process all limit the potential applications of these materials, particularly in biomedical contexts. This review highlights recent developments in the field of structured macroporous hydrogels aiming to increase network strength, create anisotropy and directionality within the networks, and utilize solvent‐free or additive‐free fabrication methods. Such functional materials are well suited for not only biomedical applications like tissue engineering and drug delivery but also selective filtration, environmental sorption, and the physical templating of secondary networks.

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Section: Electrospinningmentioning

confidence: 99%

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Hoare

2017

Adv Healthcare Materials

155

165

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“…employed in‐line blending to fabricate gelatin/polyurethane scaffolds for wound dressing applications . In‐line blending also permits generation of gradients across the depth of a scaffold . By connecting two solutions in parallel and decreasing the flow of solution 1 while increasing the flow rate of solution 2, groups have been able to establish a steady compositional gradient between the solutions.…”

Section: Electrospinning Modificationsmentioning

confidence: 99%

“…Co‐electrospinning is often conducted upon a rotating mandrel which enables the formation of gradients perpendicular to fiber alignment. To fabricate scaffolds with gradients in the direction of fiber alignment, the in‐line blending and gap electorspinning approaches (mentioned above) were combined . The in‐line blending approach enabled a temporal gradient, which was then electrospun onto a wheel of parallel copper wires that rotated at a rate synced to that of the produced gradient.…”

Section: Electrospinning Modificationsmentioning

confidence: 99%

“…60 In-line blending also permits generation of gradients across the depth of a scaffold. 14,61 By connecting two solutions in parallel and decreasing the flow of solution 1 while increasing the flow rate of solution 2, groups have been able to establish a steady compositional gradient between the solutions. Sundararaghavan et al investigated this method to create a cellular adhesive gradient (through inclusion of RGD peptide) to direct cellular migration into the depth of the mesh.…”

Section: Effects Of Modulating Solution and Processing Parametersmentioning

confidence: 99%

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Recent advancements in electrospinning design for tissue engineering applications: A review

Kishan

Cosgriff‐Hernandez

2017

J Biomedical Materials Res

Self Cite

194

132

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Electrospinning, a technique used to fabricate fibrous scaffolds, has gained popularity in recent years as a method to produce tissue engineered grafts with architectural similarities to the extracellular matrix. Beyond its versatility in material selection, electrospinning also provides many tools to tune the fiber morphology and scaffold geometry. Recent efforts have focused on extending the capabilities of electrospinning to produce scaffolds that better recapitulate tissue properties and enhance regeneration. This review highlights these advancements by providing an overview of the processing variables and setups used to modulate scaffold architecture, discussing strategies to improve cellular infiltration and guide cell behavior, and providing a summary of electrospinning applications in tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2892-2905, 2017.

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“…[8][9][10][11] On the other hand, random nanofibers are better suited for the repair of disorganized tissues such as bone, skin, and cartilage. [18,19] It was reported that the electrospun nanofibers in a nonwoven mat could be welded at their cross points when the sample was exposed to the vapor from a solvent. [15][16][17] However, the effectiveness of this class of scaffolds is compromised by the lack of a continuous gradation between the uniaxially aligned and random nanofibers.…”

Section: Doi: 101002/marc201900579mentioning

confidence: 99%

Transforming Nanofiber Mats into Hierarchical Scaffolds with Graded Changes in Porosity and/or Nanofiber Alignment

Xue

et al. 2019

Macromol. Rapid Commun.

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A method for generating hierarchical scaffolds with graded changes in porosity and/or fiber alignment through solution‐masked, vapor‐induced welding of electrospun poly(lactic‐co‐glycolic acid) (PLGA) nanofibers is reported. The success of this method relies on the fact that the PLGA nanofibers are swollen and welded more slowly by ethanol when immersed in its aqueous solution relative to direct exposure to its vapor. For a mat composed of random nanofibers, the treatment generates a gradation in porosity (both surface and bulk), with the over‐welded region evolving from a highly porous mat into a dense film. If uniaxially aligned nanofibers are involved, however, graded changes are observed in both surface porosity and fiber alignment. When bone marrow stem cells are cultured on such a scaffold, they exhibit highly organized and random morphologies on the regions of uniaxially aligned nanofibers and dense film, respectively, with gradual changes in between. Such a scaffold shows promise in mimicking the connective tissue, such as the tendon‐to‐bone insertion, that relies on a graded transition in cell morphology from uniaxially aligned to random.

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Fabrication of macromolecular gradients in aligned fiber scaffolds using a combination of in-line blending and air-gap electrospinning

Cited by 50 publications

References 48 publications

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Recent advancements in electrospinning design for tissue engineering applications: A review

Transforming Nanofiber Mats into Hierarchical Scaffolds with Graded Changes in Porosity and/or Nanofiber Alignment

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