Fabrication of liver microtissue with liver decellularized extracellular matrix (dECM) bioink by digital light processing (DLP) bioprinting

Mao, Qijiang; Wang, Yifan; Yang, Li; Juengpanich, Sarun; Li, Wenhuan; Chen, Mingyu; Yin, Jun; Fu, Jianzhong; Cai, Xiujun

doi:10.1016/j.msec.2020.110625

Cited by 149 publications

(147 citation statements)

References 37 publications

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“…fabricated DLP‐based printed lobular liver structures, [ 92 ] and Mao et al. fabricated liver microtissue [ 95 ] with DLP‐based printing. In both studies, the authors showed that improved hepatic functions were obtained in dECM/GelMA printed constructs compared to GelMA printed control group.…”

Section: Applications Of Decm‐based Bioinksmentioning

confidence: 99%

Designing Decellularized Extracellular Matrix‐Based Bioinks for 3D Bioprinting

Abaci

Guvendiren

2020

Adv Healthcare Materials

116

101

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3D bioprinting is an emerging technology to fabricate tissues and organs by precisely positioning cells into 3D structures using printable cell‐laden formulations known as bioinks. Various bioinks are utilized in 3D bioprinting applications; however, developing the perfect bioink to fabricate constructs with biomimetic microenvironment and mechanical properties that are similar to native tissues is a challenging task. In recent years, decellularized extracellular matrix (dECM)‐based bioinks have received an increasing attention in 3D bioprinting applications, since they are derived from native tissues and possess unique, complex tissue‐specific biochemical properties. This review focuses on designing dECM‐based bioinks for tissue and organ bioprinting, including commonly used decellularization and decellularized tissue characterization methods, bioink formulation and characterization, applications of dECM‐based bioinks, and most recent advancements in dECM‐based bioink design.

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Section: Applications Of Decm‐based Bioinksmentioning

confidence: 99%

Designing Decellularized Extracellular Matrix‐Based Bioinks for 3D Bioprinting

Abaci

Guvendiren

2020

Adv Healthcare Materials

116

101

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“…Therefore, researchers have been investigating decellularised ECM as bioinks. 57 – 62 Some of the reported bioinks are derived from oesophageal, adipose, cartilage, heart, cornea or liver tissue.…”

Section: Bioinks With Decellularised Matrices For Three-dimensional Pmentioning

confidence: 99%

Decellularised scaffolds: just a framework? Current knowledge and future directions

et al. 2020

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The use of decellularised matrices as scaffolds offers the advantage of great similarity with the tissue to be replaced. Moreover, decellularised tissues and organs can be repopulated with the patient’s own cells to produce bespoke therapies. Great progress has been made in research and development of decellularised scaffolds, and more recently, these materials are being used in exciting new areas like hydrogels and bioinks. However, much effort is still needed towards preserving the original extracellular matrix composition, especially its minor components, assessing its functionality and scaling up for large tissues and organs. Emphasis should also be placed on developing new decellularisation methods and establishing minimal criteria for assessing the success of the decellularisation process. The aim of this review is to critically review the existing literature on decellularised scaffolds, especially on the preparation of these matrices, and point out areas for improvement, finishing with alternative uses of decellularised scaffolds other than tissue and organ reconstruction. Such uses include three-dimensional ex vivo platforms for idiopathic diseases and cancer modelling.

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“…Mao et al [ 128 ] developed an innovative model of hepatic microtissue, in which a significant body surface area was achieved in order to improve or restore the functionality of the organ. As part of the work, a specific bioink for liver tissue was developed by combining photosetting methacrylated gelatin (GelMA) with extracellular matrix (dECM) and hepatocytes (hiHep).…”

Section: The First Bionic Organsmentioning

confidence: 99%

“…Based on the research, it was found that dECM improves both the printability and viability of cells and the functionality of hiHep cells that spread and perform their specific functions more efficiently (albumin secretion and urea). The result of this study is the development of material suitable for printing liver micro tissues that can be used in liver tissue engineering to restore liver function [ 128 ].…”

Section: The First Bionic Organsmentioning

confidence: 99%

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Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Klak¹,

Bryniarski²,

Kowalska³

et al. 2020

Micromachines

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The technology of tissue engineering is a rapidly evolving interdisciplinary field of science that elevates cell-based research from 2D cultures through organoids to whole bionic organs. 3D bioprinting and organ-on-a-chip approaches through generation of three-dimensional cultures at different scales, applied separately or combined, are widely used in basic studies, drug screening and regenerative medicine. They enable analyses of tissue-like conditions that yield much more reliable results than monolayer cell cultures. Annually, millions of animals worldwide are used for preclinical research. Therefore, the rapid assessment of drug efficacy and toxicity in the early stages of preclinical testing can significantly reduce the number of animals, bringing great ethical and financial benefits. In this review, we describe 3D bioprinting techniques and first examples of printed bionic organs. We also present the possibilities of microfluidic systems, based on the latest reports. We demonstrate the pros and cons of both technologies and indicate their use in the future of medicine.

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Fabrication of liver microtissue with liver decellularized extracellular matrix (dECM) bioink by digital light processing (DLP) bioprinting

Cited by 149 publications

References 37 publications

Designing Decellularized Extracellular Matrix‐Based Bioinks for 3D Bioprinting

Designing Decellularized Extracellular Matrix‐Based Bioinks for 3D Bioprinting

Decellularised scaffolds: just a framework? Current knowledge and future directions

Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

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